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991.
Jorge B. Torres Eduardo M. Barros Roberta R. Coelho Rejane M. M. Pimentel 《Arthropod-Plant Interactions》2010,4(4):219-227
Zoophytophagous insects can feed on a variety of prey, plants and plant products. By studying the interactions between predatory
hemipterans and plants harbouring the prey of these insects, scientists have started to establish two potential outcomes:
(1) positive effects like the enhancement of their life history characteristics by acquiring plant contents; and (2) negative
effects mediated by plant resistance to herbivores or prey ingesting secondary plant metabolites. Despite this research, there
is a lack of information about the feeding sites of predatory hemipterans on their host plants, what they ingest from plants,
and whether they cause damage to their host plants. The results presented here indicate that the xylem is one of the feeding
sites of predatory hemipterans on plants. The dissection of predators that fed on plants with marked vessels and testing insects
for the presence of Cry protein constitutively expressed in the cytoplasm of plant cells revealed that bugs are not able to
acquire cytoplasm contents from the plant cell. In addition, we demonstrate that systemic insecticide circulating inside plants
from soil applications contaminates these predators. Our results are discussed in the context of zoophytophagous feeding behaviour
exhibited by predatory hemipterans and the use of systemic insecticides for the conservation of natural enemies. This interaction
contradicts the concept of ecological selectivity obtained for natural enemies through the placement of systemic insecticide
in the soil as a selective method of deploying chemical control and predatory hemipteran conservation within the integrated
pest management framework. 相似文献
992.
This study aims to investigate the importance of the serum factors present in the plasma of resistant Biomphalaria tenagophila snails, when transferred to susceptible conspecific. Susceptible B. tenagophila (CF) received plasma from resistant B. tenagophila (Taim), and both were later infected with Schistosoma mansoni. We noticed that the plasma transfer showed an increase on the resistance of susceptible snails of about 86% when compared to the non-immunized group (p < 0.001). 相似文献
993.
Barbosa L Silva LM Coelho PM Santos SR Fortes-Dias CL 《Memórias do Instituto Oswaldo Cruz》2006,101(6):639-643
Biomphalaria glabrata snails are major hosts for the digenetic trematoda Schistosoma mansoni, the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) of the snail. In this report, the primary culture of the APO region of B. glabrata was obtained for the first time, as well as a control culture of the ovotestis. Three different cell populations migrated easily from the explants in culture, with no need of any dispersion agent. The cells grew in suspension at an incubation temperature of 15 degrees C and the cultures were maintained viable for up to two weeks. Two of these cell populations obtained resembled cell types known to be present in the hemolymph of Biomphalaria. The availability of APO cells in culture may contribute to a better understanding of the internal defense in mollusks, in general, as well as the specific response of B. glabrata to S. mansoni infection. 相似文献
994.
Faria-Pinto P Meirelles MN Lenzi HL Mota EM Penido ML Coelho PM Vasconcelos EG 《Memórias do Instituto Oswaldo Cruz》2006,101(Z1):359-363
We have previously showed that Schistosoma mansoni ATP-diphosphohydrolase and Solanum tuberosum potato apyrase share epitopes and the vegetable protein has immunostimulatory properties. Here, it was verified the in situ cross-immunoreactivity between mice NTPDases and anti-potato apyrase antibodies produced in rabbits, using confocal microscopy. Liver samples were taken from Swiss Webster mouse 8 weeks after infection with S. mansoni cercariae, and anti-potato apyrase and TRITC-conjugated anti-rabbit IgG antibody were tested on cryostat sections. The results showed that S. mansoni egg ATP diphosphohydrolase isoforms, developed by anti-potato apyrase, are expressed in miracidial and egg structures, and not in granulomatous cells and hepatic structures (hepatocytes, bile ducts, and blood vessels). Therefore, purified potato apyrase when inoculated in rabbit generates polyclonal sera containing anti-apyrase antibodies that are capable of recognizing specifically S. mansoni ATP diphosphohydrolase epitopes, but not proteins from mammalian tissues, suggesting that autoantibodies are not induced during potato apyrase immunization. A phylogenetic tree obtained for the NTPDase family showed that potato apyrase had lower homology with mammalian NTPDases 1-4, 7, and 8. Further analysis of potato apyrase epitopes could implement their potential use in schistosomiasis experimental models. 相似文献
995.
Rapid light-response curves (RLC) of variable chlorophyll fluorescence were measured on estuarine benthic microalgae with
the purpose of characterising its response to changes in ambient light, and of investigating the relationship to steady-state
light-response curves (LC). The response of RLCs to changes in ambient light (E, defined as the irradiance level to which a sample is acclimated to prior to the start of the RLC) was characterised by constructing
light-response curves for the RLC parameters α
RLC, the initial slope, ETRm,RLC, the maximum relative electron transport rate, and E
k,RLC, the light-saturation parameter. Measurements were carried out on diatom-dominated suspensions of benthic microalgae and
RLC and LC parameters were compared for a wide range of ambient light conditions, time of day, season and sample taxonomic
composition. The photoresponse of RLC parameters was typically bi-phasic, consisting of an initial increase of all parameters
under low ambient light (E < 21–181 μmol m−2 s−1), and of a phase during which α
RLC decreased significantly with E, and the increase of ETRm,RLC and E
k,RLC was attenuated. The relationship between RLC and LC parameters was dependent on ambient irradiance, with significant correlations
being found between α
RLC and α, and between ETRm,RLC and ETRm, for samples acclimated to low and to high ambient irradiances, respectively. The decline of α
RLC under high light (Δα
RLC) was strongly correlated (P < 0.001 in all cases) with the level of non-photochemical quenching (NPQ) measured before each RLC. These results indicate
the possibility of using RLCs to characterise the steady-state photoacclimation status of a sample, by estimating the LC parameter
E
k, and to trace short-term changes in NPQ levels without dark incubation. 相似文献
996.
Two Bowman-Birk type trypsin inhibitors (CmTI(1) and CmTI(2)) were purified from Cratylia mollis seeds by acetone precipitation, ion exchange, gel filtration and reverse-phase chromatography. CmTI(1) and CmTI(2), with 77 and 78 amino acid residues, respectively, were sequenced in their entirety and show a high structural similarity to Bowman-Birk inhibitors from other Leguminosae. The putative reactive sites of CmTI(1) are a lysine residue at position 22 and a tyrosine residue at position 49. Different reactive sites, as identified by their alignment with related inhibitors, were found for CmTI(2): lysine at position 22 and leucine at position 49. The dissociation constant K(i) of the complex with trypsin is 1.4 nM. The apparent molecular mass is 17 kDa without DDT and 11 kDa with reducing agent and heating. 相似文献
997.
B. W. T. Coelho 《Systematic Entomology》2004,29(3):282-323
Abstract. The New World halictid bee genus Augochlorella (Augochlorini) is revised. Sixteen species are recognized, with five described as new: Augochlorella acarinata sp. n., A. una sp. n., A. meridionalis sp. n., A. stenothoracica sp. n. from South America, and A. karankawa sp. n. from U.S.A. The following new synonymies are proposed: Augochlorella michaelis (Vachal) with A. urania (Smith), A. edendata Michener with A. comis (Vachal); A. striata (Provancher) with Augochlorella aurata (Smith) and A. neglectula maritima Ordway with A. neglectula (Cockerell). The female of A. tredecim (Vachal) and the male of A. iopoecila Moure are described for the first time. Keys to the species are provided. Cladistic analysis of adult morphological characters corroborates Engel's hypotheses of monophyly of Augochlorella phylogenetic relationships with related genera as follow: (Augochlorella ((Ceratalictus, Pereirapis) Augochlora)). Vicariant events shown in the cladistic analyses are discussed, and an account of distribution is presented. 相似文献
998.
Morphometric data collected from 118 male and 169 female savannah baboons (Papio cynocephalus anubis) aged between birth and 5.5 years were analyzed to describe the morphology and physical growth of this species. Measurements included weight, crown-rump length, triceps circumference, and skinfolds at the neck, subscapular, suprailiac, and triceps anatomical sites. Principal components analyses were applied to the data to provide multivariate assessments of morphological patterning among the variables. These analyses resulted in the extraction of two unrotated orthogonal components that accounted for 88% of the overall sample variation. The first component accounted for 77% of the variation and represents an axis of overall body size. The second component represents an axis of shape variation that contrasts body size with fat patterning, and was interpreted as a measure of body leanness. Individual component scores were computed for determining age, gender, and age-by-gender interaction effects. Both components were found to be age dependent for both genders. Males and females shared similar age patterning along the two components; however, gender differences did occur in patterning along the two components; however, gender differences did occur in respect to leanness. The multivariate measure of overall body size increased for both genders similarly with advancing age. Age patterning along the leanness component was described as a decrease from birth to 1 year, followed by an increase in leanness in older ages. Females had a delayed and significantly less intense increase in leanness relative to males. 相似文献
999.
1000.