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991.
992.
Vivian V. Costa Flavio A. Amaral Fernanda M. Coelho Celso M. Queiroz-Junior Bruna G. Malagoli Jose Hugo S. Gomes Fernando Lopes Kátia D. Silveira Daniela Sachs Caio T. Fagundes Lívia D. Tavares Vanessa Pinho Tarcilia A. Silva Mauro M. Teixeira Fern?o C. Braga Danielle G. Souza 《PloS one》2015,10(3)
Rheumatoid Arthritis (RA) is a chronic disease characterized by persistent inflammation and pain. Alternative therapies to reduce these symptoms are needed. Marine algae are valuable sources of diverse bioactive compounds. Lithothamnion muelleri (Hapalidiaceae) is a marine algae with anti-inflammatory, antitumor, and immunomodulatory properties. Here, we investigated the potential anti-inflammatory and analgesic activities of L. muelleri in a murine model of antigen-induced arthritis (AIA) in mice. Our results demonstrate that treatment with L. muelleri prevented inflammation and hypernociception in arthritic mice. Mechanistically, the crude extract and the polysaccharide-rich fractions of L. muelleri may act impairing the production of the chemokines CXCL1 and CXCL2, and consequently inhibit neutrophil influx to the knee joint by dampening the adhesion step of leukocyte recruitment in the knee microvessels. Altogether our results suggest that treatment with L.muelleri has a potential therapeutic application in arthritis treatment. 相似文献
993.
Maria Catarina Megumi Kasuya Irene da Silva Coelho Yutaka Tamai Toshizumi Miyamoto Takashi Yajima 《Mycoscience》2008,49(5):334-338
Pisolithus basidiomes were found under different forest trees in Hokkaido Island, Japan. These basidiomes were characterized morphologically
and molecularly. Although presenting different basidiome morphology and growing under different hosts, specimens presented
similar spores ornamentation, and diameters. These spores had coarse, crowded, and blunted spines with three to eight basidiospores
per basidium. Ribosomal DNA-based phylogenetic analysis indicated that variability of Pisolithus in this area is low. Phylogenetic analysis showed that Pisolithus analyzed in this study did not group with Pisolithus specimens from other geographical origins. These results suggest that Pisolithus from this area should be taxonomically distinguished from other Pisolithus. 相似文献
994.
995.
Geovanna Carla Zacheo Coelho Raphael da Silva Costa Cristiane Bashiyo‐Silva Fabrício Maral Silva de Souza Douglas de Castro Ribeiro Jos Augusto Senhorini Rosicleire Verissimo‐Silveira Alexandre Ninhaus‐Silveira 《Zeitschrift fur angewandte Ichthyologie》2019,35(3):719-727
Aiming to provide data on the biology of Leiarius marmoratus, which will aid in its production in captivity, as well as in studies for its preservation in the environment, this work had as objectives: analyze and describe main morphological alterations during larval ontogeny of the species. We analyzed 205 individuals, obtained by induced reproduction (Colpani Pisciculture) and kept in CEPTA/ICMBIO, Pirassununga, São Paulo, Brazil. Analyses were performed from hatching moment to 30th day. The specimens were classified into two periods: larval (Stages: vitelline, pre‐flexion, flexion, post‐flexion) and juvenile. Hatched larvae showed ident chromatophores only at anterior and posterior extremities of yolk sac. The standard length ranged from 2.16 mm (yolk) to 28.84 mm (Youth). Dorsal fin rays were initially observed at flexion stage (12–14 rays). Major alterations occurred during post‐flexion/juvenile stage, when dorsal, pectoral, pelvic, anal, and caudal fins were observed and pigmentation intensified throughout the lateral region, forming bands in the body, one between the end of the head and beginning of dorsal to pelvic fin, and another one beginning at dorsal to caudal peduncle and four longitudinal at the head. 相似文献
996.
997.
998.
Agnieszka P. Lipinska Sophia Ahmed Akira F. Peters Sylvain Faugeron J. Mark Cock Susana M. Coelho 《PloS one》2015,10(10)
Sex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this study, we used the recently described sequence of the sex determining region (SDR) of the brown algal model Ectocarpus to develop novel DNA-based sex-markers for three commercially relevant kelps: Laminaria digitata, Undaria pinnatifida and Macrocystis pyrifera. Markers were designed within nine protein coding genes of Ectocarpus male and female (U/V) sex chromosomes and tested on gametophytes of the three kelp species. Seven primer pairs corresponding to three loci in the Ectocarpus SDR amplified sex-specific bands in the three kelp species, yielding at least one male and one female marker for each species. Our work has generated the first male sex-specific markers for L. digitata and U. pinnatifida, as well as the first sex markers developed for the genus Macrocystis. The markers and methodology presented here will not only facilitate seaweed breeding programs but also represent useful tools for population and demography studies and provide a means to investigate the evolution of sex determination across this largely understudied eukaryotic group. 相似文献
999.
Daniela Rosa Rui Coelho Joana Fernandez-Carvalho Miguel N. Santos 《Marine Biology Research》2017,13(3):300-313
The smooth hammerhead Sphyrna zygaena (Sphyrnidae) is a pelagic shark occasionally caught as bycatch in pelagic longline fisheries, but is one of the least studied of all pelagic sharks. Age and growth of S. zygaena was studied along a wide Atlantic region covering both the northern and southern hemispheres. Data from 304 specimens, caught between October 2009 and September 2014, ranging in size from 126 to 253?cm fork length (FL), were analysed. Growth models were fitted using the three-parameter von Bertalanffy growth function (VBGF) re-parameterized to calculate L0 (size at birth). Growth models were fitted to the sample data and data from several back-calculation models. The model fit to the quadratic modified Dahl-Lea back-calculated data seems to be the most appropriate to describe growth in this species, with resulting growth parameters of Linf?=?285?cm FL, k?=?0.09 year?1 for males and Linf?=?293?cm FL, k?=?0.09 year?1 for females. Compared with other species of the same genus, estimated growth coefficients for S. zygaena seem to fall in the low to middle range. Although further work is still needed, this study adds to knowledge of the vital life-history parameters of smooth hammerheads in the Atlantic Ocean, which can be used in the management and conservation of this species. 相似文献
1000.
Gisele S. da Silva Micheli Figueiró Claudio F. Tormena Fernando Coelho 《Journal of enzyme inhibition and medicinal chemistry》2016,31(6):1464-1470
Acetylcholinesterase inhibitors and compounds that trigger Aβ amyloid oligomerization and fibrillization represent an opportunity to discover new drug candidates to treat Alzheimer’s disease. In this work, we synthesized nine new acylhydrazones and a known one, both employing 3-carboethoxy-4-quinolone derivatives as starting materials with chemical yields ranging from 63% to 90%. We evaluated the effect of these compounds on the acetylcholinesterase (AChE) activity and the fibrillization of Aβ42 peptide. Except for one acylhydrazone, the compounds exhibited good inhibitory effect on AChE (1.2?μM?<?IC50 values?<?17?μM). They also showed a significant decrease in the thioflavin-T fluorescence emission, suggesting an inhibitory effect on the Aβ42 fibril formation. 相似文献