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671.
672.
How does the post‐fire facultative seeding strategy impact genetic variation and phylogeographical history? The case of Bossiaea ornata (Fabaceae) in a fire‐prone,mediterranean‐climate ecosystem
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673.
Theodor W. Guentert Peter E. Coates Robert A. Upton Daniel L. Combs Sidney Riegelman 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1979,162(1):59-70
A specific and precise assay, capable of quantitating in human plasma simultaneously but separately quinidine, dihydroquinidine and the quinidine metabolites 2′-quinidinone, 3-OH-quinidine and a third metabolite found — tentatively identified as the product formed by rearrangement of quinidine-N-oxide — is reported. The assay uses a normal phase high-performance liquid chromatographic (HPLC) system with a variable-wavelength UV detector at 235 nm and has a limit of sensitivity at approximately 20 ng/ml. The mobile phase consists of hexanes—ethanol—ethanolamine (91.5:8.47:0.03). A 2-ml plasma sample is worked up by adding primaquine base as an internal standard and extracting with ether—dichloromethane—isopropanol (6:4:1). The organic extract is evaporated and the residue reconstituted in 100—600 μl of mobile phase and an aliquot injected onto the column.Comparison of this procedure with the Edgar and Sokolow (dichloroethane) extraction—fluorescence procedure and with the Cramer and Isaksson (benzene) double extraction—fluorescence assay indicates that both fluorescence procedures give quinidine concentrations up to 2.3 times those determined by HPLC. These discrepancies were shown to be due to carry-over of metabolites and some extraneous background fluorescence. 相似文献
674.
1. If the concentration of choline esterase is determined at different sections from the head to the caudal end of the electric organ of Electrophorus electricus (Linneaus) S-like curves are obtained. These curves are essentially the same as those which show the number of electric discs per centimeter and the E.M.F. per centimeter. 2. In the organ of Hunter the concentration of the enzyme does not differ from that in the adjacent parts in the main organ. This again coincides with the observations on the number of plates per centimeter in this organ. 3. The concentration of the enzyme was determined in different parts of the brain and the spinal cord and compared with that in a gold fish. The concentrations here are of the same order, but in the spinal cord of the eel the concentration is even lower than in the gold fish. As the cell bodies of the nerves innervating the electric organ in the spinal cord, these results do not lend support to the assumption of a special concentration of the enzyme in these nerves. 4. In the muscles adjacent to the electric organ an enzyme concentration has been found which is of the order of that in the electric tissue itself and much higher than in ordinary striated muscles. 5. The suitability of the organ for the preparation of enzyme solutions has been investigated and compared with that of the organ of Torpedo. 相似文献
675.
Dianne K. Newman Erin K. Kennedy John D. Coates Dianne Ahmann Debra J. Ellis Derek R. Lovley François M. M. Morel 《Archives of microbiology》1997,168(5):380-388
A newly discovered arsenate-reducing bacterium, strain OREX-4, differed significantly from strains MIT-13 and SES-3, the
previously described arsenate-reducing isolates, which grew on nitrate but not on sulfate. In contrast, strain OREX-4 did
not respire nitrate but grew on lactate, with either arsenate or sulfate serving as the electron acceptor, and even preferred
arsenate. Both arsenate and sulfate reduction were inhibited by molybdate. Strain OREX-4, a gram-positive bacterium with a
hexagonal S-layer on its cell wall, metabolized compounds commonly used by sulfate reducers. Scorodite (FeAsO42· H2O) an arsenate-containing mineral, provided micromolar concentrations of arsenate that supported cell growth. Physiologically
and phylogenetically, strain OREX-4 was far-removed from strains MIT-13 and SES-3: strain OREX-4 grew on different electron
donors and electron acceptors, and fell within the gram-positive group of the Bacteria, whereas MIT-13 and SES-3 fell together
in the ɛ-subdivision of the Proteobacteria. Together, these results suggest that organisms spread among diverse bacterial
phyla can use arsenate as a terminal electron acceptor, and that dissimilatory arsenate reduction might occur in the sulfidogenic
zone at arsenate concentrations of environmental interest. 16S rRNA sequence analysis indicated that strain OREX-4 is a new
species of the genus Desulfotomaculum, and accordingly, the name Desulfotomaculum auripigmentum is proposed.
Received: 22 October 1997 / Accepted: 16 June 1997 相似文献
676.
677.
678.
Alan D. Borthwick Keith Biggadike Ian L. Paternoster Jonathan A. V. Coates David J. Knight 《Bioorganic & medicinal chemistry letters》1993,3(12):2577-2580
The chiral carbocyclic nucleosides 2 and 3 were prepared from aristeromycin. The 4′-hydroxy compound 2 displays good antiviral activity against HSV-1 and HSV-2 with low toxicity. 相似文献
679.
Aminopeptidase P (AP-P; X-Pro aminopeptidase; EC 3.4.11.9) cleaves the N-terminal X-Pro bond of peptides and occurs in mammals as both cytosolic and plasma membrane forms, encoded by separate genes. In mammals, the plasma membrane AP-P can function as a kininase, but little is known about the physiological role of the cytosolic enzyme. The C. elegans genome contains a single gene encoding AP-P (W03G9.4), analysis of which predicts regions displaying high levels of amino-acid sequence homology between the predicted gene product and mammalian cytoplasmic AP-P, with the absolute conservation of key catalytic residues. The sequence of an EST (yk91g4), comprising the open reading frame of W03G9.4, confirmed the predicted genomic structure of the gene and the prediction that W03G9.4 codes for a nonsecreted protein with a molecular mass of 68 kDa. Nematodes transformed with a promoter reporter construct, W03G9.4:GFP, showed high levels of fluorescence in the intestine of larvae and adult hermaphrodites, indicating that the intestine is a major site of W03G9.4 expression. yk91g4 tagged with a hexahistidine and DLYDDDDK peptide epitope was expressed in Escherichia coli to yield, after affinity purification, a recombinant protein with a molecular mass of 71 kDa. The recombinant W03G9.4 removed the N-terminal amino acid from bradykinin (RPPGFSPFR), a Caenorhabditis elegans neuropeptide (KPSFVRFamide) and Lem Trp 1 (APSGFLGVRamide), but did not display activity towards angiotensin I (NRVYIHPFHL), des-Arg bradykinin and AF1 (KNEFIRFamide). The activity towards bradykinin was inhibited by EDTA and 1, 10 phenanthroline, as expected for a metalloenzyme, and also by apstatin (IC50, 1 microM), a selective inhibitor of mammalian AP-P. A Km of 45 microM and an optimum pH of 7-8 was observed with bradykinin as the substrate. The activity of the nematode AP-P, like its mammalian counterparts, was strongly influenced by metal ions, with Co2+, Mn2+ and Zn2+ all inhibiting the hydrolysis of bradykinin. We conclude that W03G9.4 codes for a cytoplasmic AP-P with very similar enzymatic properties to those of mammalian AP-P, and we suggest that the enzyme has a physiological role in the intracellular hydrolysis of proline-containing peptides absorbed from the lumen of the intestine. 相似文献
680.