全文获取类型
收费全文 | 309篇 |
免费 | 56篇 |
出版年
2018年 | 5篇 |
2016年 | 5篇 |
2014年 | 5篇 |
2013年 | 3篇 |
2012年 | 11篇 |
2011年 | 8篇 |
2010年 | 3篇 |
2009年 | 10篇 |
2008年 | 7篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 10篇 |
2004年 | 9篇 |
2003年 | 4篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 5篇 |
1992年 | 14篇 |
1991年 | 14篇 |
1990年 | 8篇 |
1989年 | 16篇 |
1988年 | 10篇 |
1987年 | 12篇 |
1986年 | 14篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 17篇 |
1981年 | 3篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1969年 | 6篇 |
1968年 | 4篇 |
1967年 | 3篇 |
1966年 | 7篇 |
1965年 | 4篇 |
排序方式: 共有365条查询结果,搜索用时 31 毫秒
51.
52.
Wiebke Garrels Ayan Mukherjee Stephanie Holler Nicole Cleve Thirumala R. Talluri Brigitte Barg-Kues Mike Diederich Peter Köhler Björn Petersen Andrea Lucas-Hahn Heiner Niemann Zsuzsanna Izsvák Zoltán Ivics Wilfried A. Kues 《Transgenic research》2016,25(1):63-70
Recently, we established the Sleeping Beauty transposon system for germ line competent transgenesis in the pig. Here, we extend this approach to re-target a transposon-tagged locus for a site-specific gene knock-in, and generated a syngeneic cohort of piglets carrying either the original transposon or the re-targeted event. A Cre-loxP-mediated cassette exchange of the tagging transposon with a different reporter gene was performed, followed by flow cytometric sorting and somatic cell nuclear transfer of recombined cells. In parallel, the original cells were employed in somatic cell nuclear transfer to generate clone siblings, thereby resulting in a clone cohort of piglets carrying different reporter transposons at an identical chromosomal location. Importantly, this strategy supersedes the need for an antibiotic selection marker. This approach expands the arsenal of genome engineering technologies in domestic animals, and will facilitate the development of large animal models for human diseases. Potentially, the syngeneic cohort of pigs will be instrumental for vital tracking of transplanted cells in pre-clinical assessments of novel cell therapies. 相似文献
53.
54.
Haffner CD Diaz CJ Miller AB Reid RA Madauss KP Hassell A Hanlon MH Porter DJ Becherer JD Carter LH 《Bioorganic & medicinal chemistry letters》2008,18(15):4360-4363
We report the synthesis and in vitro activity of a series of novel pyrrolidinyl pyridones and pyrazinones as potent inhibitors of prolyl oligopeptidase (POP). Within this series, compound 39 was co-crystallized within the catalytic site of a human chimeric POP protein which provided a more detailed understanding of how these inhibitors interacted with the key residues within the catalytic pocket. 相似文献
55.
Hypoxia is a widespread environmental stressor that affects marine, estuarine and freshwater systems worldwide. Investigating the effects of hypoxia on aquatic animals in the natural environment is difficult and expensive. Laboratory experiments provide an alternative that allows manipulation of environmental variables and simulation of altered conditions that are expected in the future. However, controlling dissolved oxygen (DO) levels precisely in laboratory test situations is challenging and generally costly and time intensive. In this paper, we describe a novel chamber design that is capable of maintaining low DO levels precisely for a period of at least 4 days with minimal re-adjustment and nitrogen gas requirement. The system is simple, inexpensive and easy to use, and offers the additional benefit of being portable. The utility of this design is demonstrated with an experiment to test the effects of hypoxia and high salinity on hatch rates and yolk-sac larvae survival in the estuarine fish black bream Acanthopagrus butcheri (Munro, 1949). The results show that early life-stage black bream are sensitive to hypoxia and increased salinity, indicating that deterioration in estuarine environmental conditions may have negative effects on spawning success and recruitment to fish populations. This new laboratory technique will be useful for testing future scenarios for the estuaries of southern Australia as predicted by climate change models. Other potential applications and modifications for further development of this new technique are discussed. 相似文献
56.
57.
58.
59.
60.
Mark T. Gladwin Robyn J. Barst J. Simon R. Gibbs Mariana Hildesheim Vandana Sachdev Mehdi Nouraie Kathryn L. Hassell Jane A. Little Dean E. Schraufnagel Lakshmanan Krishnamurti Enrico Novelli Reda E. Girgis Claudia R. Morris Erika Berman Rosenzweig David B. Badesch Sophie Lanzkron Oswaldo L. Castro James G. Taylor VI Jonathan C. Goldsmith Gregory J. Kato Victor R. Gordeuk Roberto F. Machado 《PloS one》2014,9(7)