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Herpes virus aotus: a latent herpesvirus from owl monkeys (Aotus trivirgatus) isolation and characterization 总被引:3,自引:0,他引:3
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The cooling and thawing rates at fixed sites within an expanding ice ball have been recorded using a temperature probe containing six integral thermocouples. The cell changes relevant to these sites have been followed through the 48 hr postfreeze-thaw period by light and electron microscopy. The results suggest that cooling rates that are compatible with cell survival in cell suspension are associated with uniform tissue cellular death in an organized tissue. 相似文献
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An improved assay for long-chain acyl-CoA synthetase 总被引:1,自引:0,他引:1
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Investigations on vitamin D esters synthesized in rats. Turnover and sites of synthesis 总被引:5,自引:5,他引:0 下载免费PDF全文
1. The tissue contents of vitamin D alcohol and ester were estimated in rats 5, 10, 24, 48 and 72hr. after peroral administration of [1-(3)H]cholecalciferol. 2. The total vitamin D in liver decreased in an exponential fashion from 19% of the dose at 5hr. to 0.6% at 72hr., but the ester content remained at a relatively constant low value from 5hr., so that by 72hr. it represented 67% of the total vitamin D. Vitamin D ester in kidney increased slowly to 48hr., but by 72hr. it was only 10% of the total vitamin D. 3. The small intestine, unlike liver and kidney, contained a higher content of vitamin D ester 10hr. after administration than at later times, and it is postulated that some vitamin D was esterified during absorption from the alimentary tract. 4. Plasma contained vitamin D ester at all time intervals, and it is suggested that ester found in liver and kidney could have been transported to these sites in the blood. 5. Thoracic-duct lymph was found to transport 43% of a peroral dose of vitamin D in 12hr., of which 1.4% was esterified. The fatty acid components of the lymph vitamin D ester, determined by two-dimensional thinlayer chromatography, were mainly palmitate (31%), stearate (25%), oleate (16%) and linoleate (16%). This pattern was similar to that previously found in liver. 相似文献
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