Bloodwood: the composition and secreting-site of the characteristic red exudate that gives the name to the Swartzia species (Fabaceae)

Journal of Plant Research - The Swartzia species are commonly known as bloodwood due to the red exudate released from the stem after injury. This exudate has aroused great...     

SASBDB: Towards an automatically curated and validated repository for biological scattering data

Small‐angle scattering (SAS) of X‐rays and neutrons is a fundamental tool to study the nanostructural properties, and in particular, biological macromolecules in solution. In structural biology, SAS recently transformed from a specialization into a general technique leading to a dramatic increase in the number of publications reporting structural models. The growing amount of data recorded and published has led to an urgent need for a global SAS repository that includes both primary data and models. In response to this, a small‐angle scattering biological data bank (SASBDB) was designed in 2014 and is available for public access at www.sasbdb.org . SASBDB is a comprehensive, free and searchable repository of SAS experimental data and models deposited together with the relevant experimental conditions, sample details and instrument characteristics. SASBDB is rapidly growing, and presently has over 1,000 entries containing more than 1,600 models. We describe here the overall organization and procedures of SASBDB paying most attention to user‐relevant information during submission. Perspectives of further developments, in particular, with OneDep system of the Protein Data Bank, and also widening of SASBDB including new types of data/models are discussed.     

Technical Procedures for the Study of the Nucleolonema

In a recent paper¹ the authors have shown filamentous structures within the nucleolus of all cells. This structure was named the nucleolonema. In the present paper the technical procedures which prove their existence are described in detail.

There are two groups: a) Silver impregnation procedures and iron-pyrogallic stain for fixed material; b) phase contrast microscopy, dark-field illumination and oblique transillumination for fresh material.

The suitable objects are mentioned in every case in order to obtain the best results.     

69,XXX karyotype in a malformed liveborn female. Maternal origin of triploidy

A liveborn female with a 69,XXX karyotype and clinical features of triploidy syndrome is reported. Main phenotypical features are: intrauterine growth retardation, hypotonicity, micrognathism, low-set ears, ocular anomalies, syndactyly and atrophy of the cerebral cortex and corpus callosum. Study of chromosomal heteromorphisms revealed that triploidy might have arisen through fertilization of a diploid ovum by a haploid sperm (diginy).     

Reconsidering anion inhibitors in the general context of drug design studies of modulators of activity of the classical enzyme carbonic anhydrase

Inorganic anions inhibit the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1) generally by coordinating to the active site metal ion. Cyanate was reported as a non-coordinating CA inhibitor but those erroneous results were subsequently corrected by another group. We review the anion CA inhibitors (CAIs) in the more general context of drug design studies and the discovery of a large number of inhibitor classes and inhibition mechanisms, including zinc binders (sulphonamides and isosteres, dithiocabamates and isosteres, thiols, selenols, benzoxaboroles, ninhydrins, etc.); inhibitors anchoring to the zinc-coordinated water molecule (phenols, polyamines, sulfocoumarins, thioxocoumarins, catechols); CAIs occluding the entrance to the active site (coumarins and derivatives, lacosamide), as well as compounds that bind outside the active site. All these new chemotypes integrated with a general procedure for obtaining isoform-selective compounds (the tail approach) has resulted, through the guidance of rigorous X-ray crystallography experiments, in the development of highly selective CAIs for all human CA isoforms with many pharmacological applications.     

Session-to-session variations in external load measures during small-sided games in professional soccer players

The aims of this study were 1) to analyse session-to-session variations in different external load measures and 2) to examine differences in within-session intervals across different small-sided game (SSG) formats in professional players. Twenty professional soccer players (mean ± SD; age 28.1 ± 4.6 years, height 176.7 ± 4.9 cm, body mass 72.0 ± 7.8 kg, and body fat 10.3 ± 3.8%) participated in 3v3, 4v4, and 6v6 SSGs under different conditions (i.e., touch limitations and presence of goalkeepers vs. free touch and ball possession drill) over three sessions. Selected external load measures—including total distance (TD), high-intensity running (HIR, distance covered > 14.4 km^.h^-1), high-speed running (HSR, distance covered > 19.8 km^.h^-1), and mechanical work (MW, accelerations and deceleration > 2.2 m^.s²)—were recorded using GPS technology during all SSG sessions. Small to large standardized typical errors were observed in session-to-session variations of selected measures across SSGs. TD^.min^-1 showed less variability, having a coefficient of variation (CV) of 2.2 to 4.6%, while all other selected external load measures had CV values ranging from 7.2% to 29.4%. Trivial differences were observed between intervals in TD^.min^-1 and HIR^.min^-1 for all SSGs, as well as in HSR^.min^-1 and MW^.min^-1 for most SSG formats. No reductions or incremental trends in session-to-session variations were observed when employing touch limitations or adding goalkeepers. The increased noise observed in higher speed zones (e.g., high-speed running) suggests a need for more controlled, running-based conditional drills if the aim is greater consistency in these measures.     

A systematic review of collective tactical behaviour in futsal using positional data

Although many studies on collective tactical behaviour have been published in the last decade, no study has revised and summarized the findings provided for futsal. The main aim of this systematic review was to identify and discuss the geometrical centre (GC), distance and area tactical variables used to assess team behaviour in futsal. In addition, it summarizes the findings on the tactical response during futsal competition and training. A systematic review of the relevant articles provided on futsal was carried out using seven electronic databases (SPORTDiscus, ProQuest, Cochrane Plus, Scopus, Google Scholar, PubMed and Web of Science) until September 25, 2019. From a total of 1,209 studies initially found, 12 were included in the qualitative synthesis. There were some trends in the analysis of positional data in futsal with the most relevant situations analysed being 1 vs 1 and 5 vs 4+Goalkeeper. The distances and angles between two points were the most assessed tactical variables. Five types of distance variables were used to assess collective tactical behaviour in futsal: GC-GC, GC-player, player-player, player-ball and player-space. Pressure (GC-GC) was greater in shots on goal than in tackles during professional futsal matches. Area variables were reduced to occupied space, exploration space and dominant area. Occupied space was measured only during competition while the dominant area was measured only during training sessions. The surface area and dominant regions were greater when players were attacking in comparison to when they were defending. In addition, two non-linear techniques (i.e. relative phase and entropy) were applied to analyse synchronisation and complexity and regularity or predictability. Defenders were highly synchronous, while attackers tried to break this coordination to achieve possibilities for action. Task constraints are suitable to induce different regularity patterns. This review is an opportunity to develop studies aimed at bridging the gap in collective tactical behaviour in futsal.