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931.
At various times after the intraperitoneal injection of Na acetate-1-C14 to male Wistar rats, the labelled fatty acids are nonuniformely distributed among the lipids of liver microsomes, mitochondria and cell sap. The changes observed in the specific radioactivity of the neutral and phospholipids support the hypothesis that a transfer of these lipids takes place from the site of synthesis (endoplasmic reticulum) to mitochondria and cell sap. This phenomenon is probably responsible for the decline of microsomal fatty acids in favour of the mitochondrial and soluble fractions. In this connection, the deacylation-reacylation process does not seem to be involved. 相似文献
932.
Some Physiological Characteristics of Two Sets of Phage-Propagating Strains of Staphylococcus aureus 下载免费PDF全文
A number of physiological characteristics were studied on some 29 strains of phage-propagating staphylococci belonging to the Basic International Series and the Seto-Wilson bovine-adapted set. All the cultures except strain 73 were coagulase-positive, with reciprocal titers ranging from 2 to 8,192. Strain 73 was again an exception with respect to phosphatase activity. Group 1 yielded high values for both phosphatase and oxygen uptake but low values for extracellular protein. Resistance to penicillin was demonstrated only by strains 80, 81, 53, 54, 75, and 77. Strain 70, one of the highest coagulase producers, alone showed no catalase activity. Mannitol was fermented by all coagulase-positive strains. Hemolysis of one or more of three kinds of erythrocytes (sheep, rabbit, and human) was a common characteristic of most strains. However, pigmentation was a nondiscriminating parameter. Although one-half of the cultures liquefied gelatin, most of them gave similar antibiotic-sensitivity tests, except the six which were penicillinase producers. There was little difference in growth rate for all strains. Comparison of coagulase production to cell size indicated that the high-titer strains were generally larger than the low producers. The foregoing evidence avers that, in addition to lytic spectrum, physiological properties can usefully characterize staphylococcal phage-propagating strains. 相似文献
933.
Different phosphorylated forms of an insulin-sensitive glycosylphosphatidylinositol from rat hepatocytes 总被引:1,自引:0,他引:1
I Merida F J Corrales R Clemente J M Ruiz-Albusac M Villalba J M Mato 《FEBS letters》1988,236(1):251-255
Labeling with [3H]galactose was employed to isolate a glycosylphosphatidylinositol from rat hepatocytes which might be involved in the action of insulin. The polar head group of this glycosylphosphatidylinositol was generated by phosphodiesterase hydrolysis with a phosphatidylinositol-specific phospholipase C from Bacillus cereus. By Dowex AG1 x 8 chromatography the polar head group could be separated into three radioactive peaks eluting at 100 mM (peak I), 200 mM (peak II) and 500 mM (peak III) ammonium formate, respectively. Peak III was the most active as an inhibitor of the cAMP-dependent protein kinase. Treatment of peak III with alkaline phosphatase markedly reduced its activity on cAMP-dependent protein kinase. When peaks I, II or III were treated with alkaline phosphatase and analyzed again by Dowex AG1 x 8 chromatography, the radioactivity eluted with the aqueous fraction. The above results indicate that the polar head group of the insulin-sensitive glycosylphosphatidylinositol from rat hepatocytes exists in three different phosphorylated forms and that the biological activity of this molecule depends on its phosphorylation state. 相似文献
934.
Kramer-Guth Annette; Busch Gillian L.; Kaba Nubia Kristen; Schwedler Susanne; Wanner Christoph; Lang Florian 《American journal of physiology. Cell physiology》1997,273(4):C1409
The present study has been performed to elucidate a possiblerole of cell volume in low-density lipoprotein (LDL) binding andinternalization (LDLb+i). Asshown previously, increase of extracellular osmolarity (OSMe) andK+ depletion, both known to shrinkcells, interfere with the formation of clathrin-coated pits and thuswith LDLb+i. On the other hand,alterations of cell volume have been shown to modify lysosomal pH,which is a determinant of LDLb+i.LDLb+i have been estimated fromheparin-releasable (binding) or heparin-insensitive (internalization)uptake of 125I-labeled LDL. OSMewas modified by alterations of extracellular concentrations of ions,glucose, urea, or raffinose. When OSMe was altered by varying NaClconcentrations, LDLb+i decreased (by 0.5 ± 0.1%/mM) with increasing OSMe andLDLb+i increased (by 1.2 ± 0.1%/mM) with decreasing OSMe, an effect mainly due to alteredaffinity; the estimated dissociation constant amounted to 20.6, 48.6, and 131.6 µg/ml at 219, 293, and 435 mosM, respectively. A 25%increase of OSMe increased cytosolic (by 0.46 ± 0.03) and decreasedlysosomal (by 0.14 ± 0.02) pH. Conversely, a 25% decrease of OSMedecreased cytosolic (by 0.28 ± 0.02) and increased lysosomal (by0.17 ± 0.02) pH. Partial replacement of extracellularNa+ withK+ had little effect onLDLb+i, although it swelledhepatocytes and increased lysosomal and cytosolic pH. Hypertonicglucose, urea, or raffinose did not exert similar effects despite ashrinking effect of hypertonic raffinose. Monensin, which completelydissipates lysosomal acidity, virtually abolishedLDLb+i. In conclusion, theobservations reveal a significant effect of ionic strength onLDLb+i. The effect is, however,not likely to be mediated by alterations of cell volume or alterationsof lysosomal pH. 相似文献
935.
Ekaterina Shumilina Naima Zahir Nguyen Thi Xuan Florian Lang 《Cellular physiology and biochemistry》2007,20(6):801-808
The phosphoinositide 3 (PI3) kinase plays a pivotal role in the regulation of dendritic cells (DCs), antigen-presenting cells that are able to initiate primary immune responses and to establish immunological memory. PI3 kinase is an endogenous suppressor of interleukin 12 (IL-12) production in DCs that is triggered by Toll-like receptor signaling. Inhibition of IL-12 production limits T helper 1 (Th1) polarization. On the other hand, PI3 kinase is an important regulator of various ion channels. The present study aimed to explore whether ion channels in DCs are regulated by PI3 kinase and whether they are important for DC function. To this end, DCs were isolated from murine bone marrow and ion channel activity was determined by patch clamp. As a result, DCs express voltage-gated K(+) channels (Kv), which are blocked by Stichodactyla helianthus toxin (ShK, 2.5 nM). A significant upregulation of Kv currents was observed upon maturation of DCs as induced by stimulation of the cells with lipopolysaccharide (LPS, 0.1 microg/ml, 48 h). A dramatic increase of Kv current amplitude was observed following preincubation of the cells with LY294002 (100 nM), a specific inhibitor of PI3 kinase. PI3 kinase inhibitor wortmannin (100 nM) similarly increased Kv current. LY294002 treatment was further followed by a significant increase of IL-12 production. ShK (100 nM) significantly blunted the stimulation of IL-12 release by LPS but not when the cells were first pretreated with LY294002. The observations point to Kv channel sensitive and Kv channel insensitive regulation of DC function. 相似文献
936.
Tissue-specific disruption of genes by targeted expression of Cre recombinase in insulin-producing cells has been widely used to explore pathways involved in regulation of pancreatic beta-cell mass. One particular line of transgenic mice [B6.Cg-Tg(Ins2-cre)25Mgn/J], commonly called RIP-Cre, in which the expression of Cre recombinase is controlled by a short fragment of the rat insulin II gene promoter has been used on at least 20 genes in at least 27 studies. In the majority of these studies (15 out of 27) inactivation of the gene of interest was associated with alterations in islet architecture, islet mass, or pancreatic insulin content. We have tested the hypothesis that genomic integration or expression of Cre recombinase alone causes alterations of beta-cell mass by quantifying islet number and mass in RIP-Cre mice. We have observed a significant hypoplasia of beta-cells in young RIP-Cre mice, and a significant hyperplasia of islets in older RIP-Cre animals. These findings suggest that glucose intolerance and impaired insulin secretion previously described for younger RIP-Cre mice might be caused by transgene-associated islet hypoplasia, and that hyperplasia in older mice might reflect a compensatory response to transgene-related glucose intolerance. 相似文献
937.
Antonio Peña Salvador Uribe Mónica Clemente Norma Sánchez 《Archives of microbiology》1992,158(2):75-80
Respiration and fermentation were lower in active dry yeast (ADY) rehydrated at 0°C than in ADY rehydrated at 40°C. In agreement with other reports, it was found that membrane permeability increased during rehydration. In addition, ADY rehydrated at 0° did not reseal, even after hours of incubation at 40°C. Using 32P-nuclear magnetic resonance it was found that the cellular concentration of sugar phosphates, phosphate, pyrophosphate, NADH and ATP were lower in ADY rehydrated at 0°C. In addition, the phospholipid peak had a higher height to broadness ratio at 0°C than at 40°C, suggesting that membranes in the 0° sample were more disordered. The lower fermentation rate in ADY rehydrated at 0° could not be due solely to membrane permeation since addition of cofactors that leaked from these cells did not reactivate fermentation. In cell free extracts or in toluenized cells it was observed that some activities were modified after rehydration at 0°C. In the 40°C sample a lower activity of pyruvate decarboxylase and higher fructose-1,6-bisphosphatase and ATPase activities were detected. As a result, higher levels of ADP and pyruvate were found in the cell. Higher ADP levels could contribute to the higher fermentation rate of the cells rehydrated at 40°C. Enzyme modification might explain the low viability of ADY observed by a plating method, even in cells that were impermeable to a vital dye.Abbreviations ADY
Active dry yeast
- MES
2(N-morpholino)-ethanesulfonic acid 相似文献
938.
939.
Patrick Rukavina Fritz Koch Maximilian Wehrle Kevin Tröndle G. Björn Stark Peter Koltay Stefan Zimmermann Roland Zengerle Florian Lampert Sandra Strassburg Günter Finkenzeller Filip Simunovic 《Biotechnology and bioengineering》2020,117(12):3902-3911
Bioprinting can be considered as a progression of the classical tissue engineering approach, in which cells are randomly seeded into scaffolds. Bioprinting offers the advantage that cells can be placed with high spatial fidelity within three-dimensional tissue constructs. A decisive factor to be addressed for bioprinting approaches of artificial tissues is that almost all tissues of the human body depend on a functioning vascular system for the supply of oxygen and nutrients. In this study, we have generated cuboid prevascularized bone tissue constructs by bioprinting human adipose-derived mesenchymal stem cells (ASCs) and human umbilical vein endothelial cells (HUVECs) by extrusion-based bioprinting and drop-on-demand (DoD) bioprinting, respectively. The computer-generated print design could be verified in vitro after printing. After subcutaneous implantation of bioprinted constructs in immunodeficient mice, blood vessel formation with human microvessels of different calibers could be detected arising from bioprinted HUVECs and stabilization of human blood vessels by mouse pericytes was observed. In addition, bioprinted ASCs were able to synthesize a calcified bone matrix as an indicator of ectopic bone formation. These results indicate that the combined bioprinting of ASCs and HUVECs represents a promising strategy to produce prevascularized artificial bone tissue for prospective applications in the treatment of critical-sized bone defects. 相似文献
940.
Xiaobing Gong Yuan Gao Guoqing Guo Florian W. R. Vondran Ruth Schwartlander Ekaterina Efimova Gesine Pless Igor M. Sauera Peter Neuhaus 《Cytotechnology》2015,67(2):255-265
Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocytes were employed to elucidate the protective effects and molecular mechanisms of matrine. We observed that low concentrations of matrine had no significant impact on albumin secretion, but high concentrations (>140 mg/L) of matrine decreased the albumin secretion in hepatocytes. Western blot data indicated that matrine at 140 mg/L at 72 h induced protein expression of CYP2A6, CYP2B6 and CYP3A4. Furthermore, high concentrations of matrine reduced LDH and AST levels and were cytotoxic to hepatocytes, leading to a decreased cell viability and total protein amount. Moreover, low concentrations of matrine, enhanced the ECOD activity and decreased the level of NO2− induced by cytokines in human hepatocytes. Taken together, the present study sheds novel light on the molecular mechanisms of matrine and potential application of matrine in hepatic diseases.