全文获取类型
收费全文 | 4182篇 |
免费 | 339篇 |
专业分类
4521篇 |
出版年
2024年 | 3篇 |
2023年 | 43篇 |
2022年 | 96篇 |
2021年 | 177篇 |
2020年 | 92篇 |
2019年 | 112篇 |
2018年 | 129篇 |
2017年 | 115篇 |
2016年 | 155篇 |
2015年 | 261篇 |
2014年 | 281篇 |
2013年 | 308篇 |
2012年 | 479篇 |
2011年 | 390篇 |
2010年 | 221篇 |
2009年 | 198篇 |
2008年 | 265篇 |
2007年 | 253篇 |
2006年 | 189篇 |
2005年 | 174篇 |
2004年 | 130篇 |
2003年 | 122篇 |
2002年 | 93篇 |
2001年 | 32篇 |
2000年 | 24篇 |
1999年 | 26篇 |
1998年 | 17篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 16篇 |
1991年 | 6篇 |
1990年 | 6篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1983年 | 6篇 |
1979年 | 5篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1971年 | 4篇 |
1969年 | 3篇 |
1968年 | 3篇 |
1967年 | 4篇 |
1963年 | 2篇 |
1959年 | 2篇 |
1955年 | 2篇 |
1933年 | 2篇 |
排序方式: 共有4521条查询结果,搜索用时 9 毫秒
901.
Adriano C. Quaresma Maria Teresa F. Piedade Florian Wittmann Hans ter Steege 《Biodiversity and Conservation》2018,27(8):1981-2002
This study examines the occurrence of vascular epiphytic species in Central Amazonian black-water floodplain forests (igapó) and considers whether their horizontal and vertical distribution is influenced by the flood pulse, as is the case with tree species (phorophytes). Research was conducted in sixteen forest plots the Jaú National Park. In these, epiphytes on all phorophytes with DBH ≥ 10 cm were identified. We measured flood height using the watermark left by the last high-water period, then estimated the height relative to the ground of every epiphytic individual. We recorded 653 individuals in 37 species, distributed on 109 phorophytes. Igapó floodplain forests have much lower richness and abundance of vascular epiphyte species than do other Amazonian forests. This may reflect the limitation of available sites for colonization (only 24.9% of studied trees were occupied by epiphytes). Holoepiphytes predominated, and the combined presence of a flood-pulse, linked to the nutrient-poor soil poor seems to limit the occurrence of nomadic vines. Horizontal distribution of epiphytes followed the distribution of phorophytes, which in turn followed the flood-level gradient. Also flooding interacted strongly with vertical zonation to determine species richness. As already well-reported for trees, and unlike reports of epiphytes in other floodplains, flooding strongly influenced richness and distribution of vascular epiphytes in the studied igapó forests. 相似文献
902.
The floral homeotic protein SEPALLATA3 recognizes target DNA sequences by shape readout involving a conserved arginine residue in the MADS‐domain 下载免费PDF全文
903.
904.
905.
Control of RAB7 activity and localization through the retromer‐TBC1D5 complex enables RAB7‐dependent mitophagy 下载免费PDF全文
Heike Nägele Justin Denner Stefan Eimer Jörn Dengjel Florian Steinberg 《The EMBO journal》2018,37(2):235-254
Retromer is an endosomal multi‐protein complex that organizes the endocytic recycling of a vast range of integral membrane proteins. Here, we establish an additional retromer function in controlling the activity and localization of the late endosomal small GTPase RAB7. Surprisingly, we found that RAB7 not only decorates late endosomes or lysosomes, but is also present on the endoplasmic reticulum, trans‐Golgi network, and mitochondrial membranes, a localization that is maintained by retromer and the retromer‐associated RAB7‐specific GAP TBC1D5. In the absence of either TBC1D5 or retromer, RAB7 activity state and localization are no longer controlled and hyperactivated RAB7 expands over the entire lysosomal domain. This lysosomal accumulation of hyperactivated RAB7 results in a striking loss of RAB7 mobility and overall depletion of the inactive RAB7 pool on endomembranes. Functionally, we establish that this control of RAB7 activity is not required for the recycling of retromer‐dependent cargoes, but instead enables the correct sorting of the autophagy related transmembrane protein ATG9a and autophagosome formation around damaged mitochondria during Parkin‐mediated mitophagy. 相似文献
906.
Jellena V. Düster Maria H. Gruber Florian Karolyi John D. Plant Harald W. Krenn 《Arthropod Structure & Development》2018,47(1):25-35
Neotropical orchid bees (Euglossini) possess the longest proboscides among bees. In this study, we compared the feeding behavior and functional morphology of mouthparts in two similarly large-sized species of Euglossa that differ greatly in proboscis length. Feeding observations and experiments conducted under semi-natural conditions were combined with micro-morphological examination using LM, SEM and micro CT techniques. The morphometric comparison showed that only the components of the mouthparts that form the food tube differ in length, while the proximal components, which are responsible for proboscis movements, are similar in size. This study represents the first documentation of lapping behaviour in Euglossini. We demonstrate that Euglossa bees use a lapping-sucking mode of feeding to take up small amounts of fluid, and a purely suctorial technique for larger fluid quantities. The mouthpart movements are largely similar to that in other long-tongued bees, except that the postmentum in Euglossa can be extended, greatly enhancing the protraction of the glossa. This results in a maximal functional length that is about 50% longer than the length of the food canal composing parts of the proboscis. The nectar uptake and the sensory equipment of the proboscis are discussed in context to flower probing. 相似文献
907.
Ecological selection of siderophore‐producing microbial taxa in response to heavy metal contamination 下载免费PDF全文
Elze Hesse Siobhán O'Brien Nicolas Tromas Florian Bayer Adela M. Luján Eleanor M. van Veen Dave J. Hodgson Angus Buckling 《Ecology letters》2018,21(1):117-127
Some microbial public goods can provide both individual and community‐wide benefits, and are open to exploitation by non‐producing species. One such example is the production of metal‐detoxifying siderophores. Here, we investigate whether conflicting selection pressures on siderophore production by heavy metals – a detoxifying effect of siderophores, and exploitation of this detoxifying effect – result in a net increase or decrease. We show that the proportion of siderophore‐producing taxa increases along a natural heavy metal gradient. A causal link between metal contamination and siderophore production was subsequently demonstrated in a microcosm experiment in compost, in which we observed changes in community composition towards taxa that produce relatively more siderophores following copper contamination. We confirmed the selective benefit of siderophores by showing that taxa producing large amounts of siderophore suffered less growth inhibition in toxic copper. Our results suggest that ecological selection will favour siderophore‐mediated decontamination, with important consequences for potential remediation strategies. 相似文献
908.
Florian Martin Niethammer Julia Ade Ludwig Eduard Hoelzle Benjamin Schade 《Acta veterinaria Scandinavica》2018,60(1):74
Background
The significance of hemotrophic mycoplasma in cattle remains unclear. Especially in Europe, their epidemiological parameters as well as pathophysiological influence on cows are lacking. The objectives of this study were: (1) to describe the prevalence of ‘Candidatus Mycoplasma haemobos’ (‘C. M. haemobos’) and Mycoplasma wenyonii (M. wenyonii) in Bavaria, Germany; (2) to evaluate their association with several blood parameters; (3) to explore the potential of vertical transmission in Simmental cattle; and (4) to evaluate the accuracy of acridine-orange-stained blood smears compared to real-time polymerase chain reaction (PCR) results to detect hemotrophic mycoplasma. A total of 410 ethylenediaminetetraacetic acid-blood samples from cows from 41 herds were evaluated by hematology, acridine-orange-stained blood smears, and real-time PCR. Additionally, blood samples were taken from dry cows of six dairy farms with positive test results for hemotrophic mycoplasma to investigate vertical transmission of infection.Results
The period prevalence of both species was 60.24% (247/410), C. M. haemobos 56.59% (232/410), M. wenyonii 8.54% (35/410) and for coinfection 4.88% (20/410). Of the relevant blood parameters, only mean cell volume (MCV), mean cell hemoglobin (MCH), and white blood cell count (WBC) showed differences between the groups of infected and non-infected individuals. There were lower values of MCV (P?<?0.01) and MCH (P?<?0.01) and higher values of WBC (P?<?0.05) in ‘C. M. haemobos’-infected cows. In contrast, co-infected individuals had only higher WBC (P?<?0.05). In M. wenyonii-positive blood samples, MCH was significantly lower (P?<?0.05). Vertical transmission of ‘C. M. haemobos’ was confirmed in two calves. The acridine-orange-method had a low sensitivity (37.39%), specificity (65.97%), positive predictive value (63.70%) and negative predictive value (39.75%) compared to PCR.Conclusions
‘Candidatus Mycoplasma haemobos’ was more prevalent than M. wenyonii in Bavarian Simmental cattle, but infection had little impact on evaluated blood parameters. Vertical transmission of the infection was rare. Real-time PCR is the preferred diagnostic method compared to the acridine-orange-method.909.
Do the fluorescent red eyes of the marine fish Tripterygion delaisi stand out? In situ and in vivo measurements at two depths 下载免费PDF全文
Ulrike K. Harant Matteo Santon Pierre‐Paul Bitton Florian Wehrberger Thomas Griessler Melissa G. Meadows Connor M. Champ Nico K. Michiels 《Ecology and evolution》2018,8(9):4685-4694
Since the discovery of red fluorescence in fish, much effort has been invested to elucidate its potential functions, one of them being signaling. This implies that the combination of red fluorescence and reflection should generate a visible contrast against the background. Here, we present in vivo iris radiance measurements of Tripterygion delaisi under natural light conditions at 5 and 20 m depth. We also measured substrate radiance of shaded and exposed foraging sites at those depths. To assess the visual contrast of the red iris against these substrates, we used the receptor noise model for chromatic contrasts and Michelson contrast for achromatic calculations. At 20 m depth, T. delaisi iris radiance generated strong achromatic contrasts against substrate radiance, regardless of exposure, and despite substrate fluorescence. Given that downwelling light above 600 nm is negligible at this depth, we can attribute this effect to iris fluorescence. Contrasts were weaker in 5 m. Yet, the pooled radiance caused by red reflection and fluorescence still exceeded substrate radiance for all substrates under shaded conditions and all but Jania rubens and Padina pavonia under exposed conditions. Due to the negative effects of anesthesia on iris fluorescence, these estimates are conservative. We conclude that the requirements to create visual brightness contrasts are fulfilled for a wide range of conditions in the natural environment of T. delaisi. 相似文献
910.
Florian Otto Andreas Mackensen Roland Mertelsmann Rupert Engelhardt 《Cancer immunology, immunotherapy : CII》1995,40(2):115-118
The course of metastatic renal cell carcinoma may be positively influenced by immunotherapeutic agents. We report a case of renal cell carcinoma showing a complete response to once-weekly low-dose s. c. interferon- (INF) treatment in multiple metastatic sites (lung, chest wall, abdomen, vertebral body), but concomitantly developing a solitary brain metastasis. High initial interleukin-6 (IL-6) levels returned to normal during IFN treatment suggesting that IFN may have interrupted an autocrine IL-6/IL-6-receptor loop of the tumor cells. The duration of complete remission in the extracerebral sites is now 46+ months. IFN may be less active beyond the blood/brain barrier. 相似文献