全文获取类型
收费全文 | 21723篇 |
免费 | 2140篇 |
国内免费 | 19篇 |
专业分类
23882篇 |
出版年
2022年 | 207篇 |
2021年 | 433篇 |
2020年 | 240篇 |
2019年 | 301篇 |
2018年 | 394篇 |
2017年 | 317篇 |
2016年 | 467篇 |
2015年 | 895篇 |
2014年 | 882篇 |
2013年 | 1217篇 |
2012年 | 1532篇 |
2011年 | 1481篇 |
2010年 | 948篇 |
2009年 | 856篇 |
2008年 | 1209篇 |
2007年 | 1240篇 |
2006年 | 1140篇 |
2005年 | 1197篇 |
2004年 | 1131篇 |
2003年 | 1054篇 |
2002年 | 1037篇 |
2001年 | 221篇 |
2000年 | 161篇 |
1999年 | 261篇 |
1998年 | 281篇 |
1997年 | 187篇 |
1996年 | 171篇 |
1995年 | 163篇 |
1994年 | 160篇 |
1993年 | 184篇 |
1992年 | 167篇 |
1991年 | 152篇 |
1990年 | 140篇 |
1989年 | 166篇 |
1988年 | 157篇 |
1987年 | 134篇 |
1986年 | 128篇 |
1985年 | 156篇 |
1984年 | 150篇 |
1983年 | 142篇 |
1982年 | 192篇 |
1981年 | 198篇 |
1980年 | 149篇 |
1979年 | 117篇 |
1978年 | 146篇 |
1977年 | 112篇 |
1976年 | 108篇 |
1975年 | 91篇 |
1974年 | 99篇 |
1973年 | 85篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
71.
Joseph Nagyvary Janos H. Fendler 《Origins of life and evolution of the biosphere》1974,5(3-4):357-362
Selective compartmentalization of amino acids and nucleotides according to their polarities is proposed as a physical-chemical model for the origin of the genetic code. Assumptions made in this hypothesis are: (1) an oil-slick covered the surface of the primitive ocean, constituents of which formed association colloids or micelles at the water-oil-air interfaces; (2) depending on the polarity of the media, these aggregates possessed hydrophilic and hydrophobic interiors where selective uptake of amino acids and nucleic acid constituents could take place; and 93) condensation and polymerization in the micellar phase were enhanced. According to the chromatographically observed polarities, for example, lysine and uridylate fall into the hydrophilic compartment, and phenylalanine and adenylate are enriched in the hydrophobic environment. These components could eventually be condensed to form a charged adaptor loop with an anticodon which is complementary to the presently valid codon. Only two groups of amino acids, hydrophilic and hydrophobic, were recognized by the primitive translation mechanism. Implications of this hypothesis for the further development of the genetic code is discussed. The catalytic power of micelles have been substantiated by successful synthesis of nucleotides under relatively mild conditions using thiophosphates as high energy phosphates. 相似文献
72.
Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo 总被引:1,自引:1,他引:0 下载免费PDF全文
Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T1 ribonuclease digest of this purified tRNA gave a simple chromatographic pattern. Because of the simplicity of the method of preparation of tRNA from this readily available source and the presence of only a few species of tRNA for each amino acid, chick embryo is suited for the study of tRNA and its various functions in higher systems. 相似文献
73.
Warren O. Kessler Ruben F. Gittes Sheldon R. Hurwitz Joseph P. Green 《The Western journal of medicine》1974,121(2):91-93
Gallium-67 (67Ga) citrate was administered intravenously (50 microcuries per kg of body weight) to patients in whom acute and chronic urinary tract infections were suspected. Scanning was done, using both the Anger-type scintillation camera and the rectilinear scanner, 24 to 78 hours after injection of the isotope.The preliminary results imply that 67Ga renal uptake is present in patients with pyelonephritis whether overt or silent, as well as in patients with uretero-sigmoidostomies. However, 67Ga renal uptake is not present in patients with radiographic evidence of chronic pyelonephritis without active infection and in patients without renal disease. 相似文献
74.
Determination of Antibiotic Susceptibility of Rickettsia by the Plaque Assay Technique 总被引:6,自引:0,他引:6 下载免费PDF全文
Joseph E. McDade 《Applied microbiology》1969,18(1):133-135
Plaque formation by various rickettsiae was completely inhibited by commercial antibiotic discs impregnated with tetracycline, chloramphenicol, nitrofurantoin, and erythromycin; partial inhibition was observed around discs containing nalidixic acid and sulfisoxazole, but no inhibition was seen around discs containing cephalothin, ampicillin, oxacillin, kanamycin, polymyxin B, streptomycin, or penicillin. 相似文献
75.
Joseph Dancis Rody P. Cox Peter H. Berman Valerie Jansen M. Earl Balis 《Biochemical genetics》1969,3(6):609-615
Radioautographic examination of skin fibroblasts grown in tissue culture from normal donors revealed heavy labeling of almost all cells following incubation with tritiated hypoxanthine. Cells from patients with Lesch-Nyhan's disease, lacking inosinate pyrophosphorylase, had only 10 grains or less per cell. When normal and abnormal cells were mixed prior to culture, there was a progressive increase, with culture time, in the percentage of heavily labeled cells so that by 96 hr, when the cells were confluent, over 95% of the cells were heavily labeled. Reduction of cell density by subculture produced a reversion to original values. Cultures from three obligatory heterozygotes revealed the expected mixed population of cells. This appears to be a practical approach to the identification of the heterozygote.Aided by USPHS CA08748 and GM15508, and the Health Research Council of the City of New York. 相似文献
76.
Effect of the Route of Inoculation on Antibody Formation in Guinea Pigs Immunized with Parainfluenza Virus Vaccines 下载免费PDF全文
Joseph L. DeMeio 《Applied microbiology》1968,16(8):1207-1210
The efficiency of the intramuscular route of immunization of guinea pigs was compared with the intraperitoneal route when trivalent parainfluenza virus vaccine was employed as immunogen. When the routes of immunization were compared by effective dose 50, the intramuscular route was more effective. Likewise, a statistical evaluation of conversion rates and of titers revealed significantly higher values for the intramuscular than for the intraperitoneal route to all three components in two of the lots of vaccine tested. 相似文献
77.
Interaction of the exr and lon Genes in Escherichia coli 总被引:6,自引:5,他引:1
Strains of Escherichia coli carrying the gene lon typically produced excess capsular polysaccharide, and were sensitive to ultraviolet light (UV) irradiation, thymine starvation, and nalidixic acid, forming long filaments after these treatments. Sensitivity was reduced by a number of posttreatments. In the presence of a second UV sensitivity gene, exr, some of these properties were suppressed: long filaments were not formed, the effect of lon on UV and nalidixic acid sensitivity was greatly reduced, and irradiation posttreatments gave an enhancement of survival characteristic of exr rather than lon strains. Production of capsular polysaccharide was not affected by the exr gene. 相似文献
78.
Joseph R. Songer 《Applied microbiology》1967,15(1):35-42
A system for studying the effects of relative humidity (RH) and temperature on biological aerosols, utilizing a modified toroid for a static aerosol chamber, is described. Studies were conducted at 23 C and at three RH levels (10, 35, and 90%) with four viruses (Newcastle disease virus, infectious bovine rhinotracheitis virus, vesicular stomatitis virus, and Escherichia coli B T3 bacteriophage). Virus loss on aerosol generation was consistently lower at 90% than at 10 or 35% RH. When stored at 23 C, Newcastle disease virus and vesicular stomatitis virus survived best at 10% RH. Infectious bovine rhinotracheitis virus and E. coli B T3 bacteriophage survived storage at 23 C best at 90% RH. 相似文献
79.
The usual assumption in treating the diffusion of ions in an electric field has been that the movement of each ion is independent of the movement of the others. The resulting equation for diffusion by a succession of spontaneous jumps has been well stated by Parlin and Eyring. This paper will consider one simple case in which a different assumption is reasonable. Diffusion of monovalent positive ions is considered as a series of jumps from one fixed negative site to another. The sites are assumed to be full (electrical neutrality). Interaction occurs by the displacement of one ion by another. An ion leaves a site if and only if another ion, not necessarily of the same species, attempts to occupy the same site. Flux ratios and net fluxes are given as functions of the electrical potential, concentration ratios, and number of sites encountered in crossing the membrane. Quantitative comparisons with observations of Hodgkin and Keynes are presented. 相似文献
80.