全文获取类型
收费全文 | 4751篇 |
免费 | 348篇 |
专业分类
5099篇 |
出版年
2021年 | 34篇 |
2020年 | 22篇 |
2019年 | 33篇 |
2018年 | 47篇 |
2017年 | 48篇 |
2016年 | 78篇 |
2015年 | 118篇 |
2014年 | 134篇 |
2013年 | 205篇 |
2012年 | 251篇 |
2011年 | 243篇 |
2010年 | 161篇 |
2009年 | 172篇 |
2008年 | 249篇 |
2007年 | 242篇 |
2006年 | 255篇 |
2005年 | 246篇 |
2004年 | 236篇 |
2003年 | 295篇 |
2002年 | 306篇 |
2001年 | 80篇 |
2000年 | 43篇 |
1999年 | 88篇 |
1998年 | 95篇 |
1997年 | 83篇 |
1996年 | 75篇 |
1995年 | 60篇 |
1994年 | 57篇 |
1993年 | 62篇 |
1992年 | 81篇 |
1991年 | 56篇 |
1990年 | 34篇 |
1989年 | 51篇 |
1988年 | 49篇 |
1987年 | 40篇 |
1986年 | 33篇 |
1985年 | 42篇 |
1984年 | 44篇 |
1983年 | 35篇 |
1982年 | 56篇 |
1981年 | 53篇 |
1980年 | 48篇 |
1979年 | 50篇 |
1978年 | 42篇 |
1977年 | 36篇 |
1976年 | 42篇 |
1975年 | 27篇 |
1974年 | 22篇 |
1973年 | 19篇 |
1971年 | 19篇 |
排序方式: 共有5099条查询结果,搜索用时 15 毫秒
81.
Heinz Egge Jean Claude Michalski Gerard Strecker 《Archives of biochemistry and biophysics》1982,213(1):318-326
Three oligosaccharides isolated from the urine of a patient suffering from mannosidosis, a Man9GlcNAc, a Man8GlcNAc, and a Man7GlcNAc, were analyzed by electron impact mass spectrometry at 20 eV after reduction with NaB2H4 and permethylation. Molecular ions were observed at 2144, 1940, and 1736, respectively. In the high-mass range very intense ions were found at M-45. The mass spectrum of the homogenous decasaccharide Man9GlcNAc1D contains ions that could be attributed to specific parts of each of the three antennae of the molecule. Thus characteristic key ions were recognized. With the aid of these key ions the spectra of the nona- and octasaccharide mixtures could be evaluated in a qualitative and a semiquantitative way. In the nonasaccharide all three possible isomers that can be produced by cleavage of one of the three terminal α-mannoses are present, although in differing amounts. However, only five of the six possible isomers of the octasaccharide could be detected. 相似文献
82.
The gap between the theoretical biological potential of microalgae and the biomass productivity obtained with algal culture in tubular biophotoreactors is due to a reduced growth rate related to hydrodynamic stress of pumping. High levels of mixing are necessary to reach a turbulent flow of the culture, in order to optimize the light regime. The optimal conditions of pumping to produce this significant liquid mixing may produce some cell damage. Factors affecting this hydrodynamic stress (geometry of the bioreactor involved, type of pump utilized, morphology of algal cells, physiological conditions of microalgae, etc.) are discussed. 相似文献
83.
Pierre Valeix Paul Preziosi Claude Rossignol Marie-Alice Farnier Serge Hercberg 《Biological trace element research》1992,32(1-3):259-266
Urinary iodine excretion was assessed in 642 healthy children aged 10 mo (n=243), 2 yr (n=183), and 4 yr (n=216) living in the Paris area and originating from continental France (60.3%), North Africa (13.8%), the West Indies (9.1%),
West Africa (8.3%), Southeast Asia (4.8%), and southern Europe (3.8%). Mild impairment of neurological (reflexes, tone, audiometry)
and intellectual development (Brunet-Lézine scale) was assessed in relation to iodine status. Iodine excretions (median values)
were 18.4, 11.9, and 10.9 μg/100 mL at 10 mo, 2 yr, and 4 yr, respectively, and risk of mild iodine deficiency (5–10 μg/100
mL) was 18.1%, 34.8%, and 38.3% for the same age groups. No relationship was found between anthropometry, global development
quotient, and iodine status. High hearing thresholds were more commonly associated with lower iodine excretion, suggesting
mild hearing defects. In spite of iodine prophylaxis, the risk of mild to moderate iodine deficiency still exists in France
and in a number of European countries. Evaluation of neurological sequels of borderline iodine status is a major public health
problem in European communities. 相似文献
84.
Carl E. Hilliker Martine I. Darville Magdy S. Aly Mohamed Chikri Claude Szpirer Peter Marynen Guy G. Rousseau Jean-Jacques Cassiman 《Genomics》1991,10(4)
Two genes encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were localized in human and rat chromosomes. PFKFB1 (previously PFRX), which encodes the liver and muscle isozymes, was assigned to Xq22-q31 in the rat and to Xq27–q28 in the human by in situ hybridization using probes generated by the polymerase chain reaction. PFKFB2, which encodes the heart isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, was assigned to chromosome 13 in the rat and to chromosome 1 in the human by hybridization of DNA from somatic cell hybrids. By in situ hybridization, this gene was localized to the regions 13q24–25 in the rat and 1q31 in the human. 相似文献
85.
86.
A quantitative autoradiographic method for the measurement of local rates of brain protein synthesis
We have developed a new method for measuring local rates of brain protein synthesis in vivo. It combines the intraperitoneal injection of a large dose of low specific activity amino acid with quantitative autoradiography. This method has several advantages: 1) It is ideally suited for young or small animals or where immobilizing an animal is undesirable. 2) The amino acid injection floods amino acid pools so that errors in estimating precursor specific activity, which is especially important in pathological conditions, are minimized. 3) The method provides for the use of a radioautographic internal standard in which valine incorporation is measured directly. Internal standards from experimental animals correct for tissue protein content and self-absorption of radiation in tissue sections which could vary under experimental conditions.A preliminary report of this work was presented at the 11th annual meeting of the Society for Neuroscience, Los Angeles, California, October 18–23, 1981. 相似文献
87.
88.
Claude Guérin 《Geobios》1982,15(4):593-598
For the Uppermost Miocene the already known zones MN 9 to MN 13 are used without any change; they prove to be particularly useful for the study of fossil rhinocerotids. The Plio-Villafranchian age corresponds to the zones 14 to 19, the definition of the first five being completed and the last being new. Middle to Upper Pleistocene age deals with the zones 20 to 26, all being new and defined following the same principles. 相似文献
89.
90.
A new spectrofluorometric method for heme quantitation in cultured fibroblasts is described. The method includes: (1) heme extraction by methanol/sulfuric acid, (2) partial purification of heme by a microchromatographic method, and (3) treatment of the purified heme by oxalic acid followed by fluorometric quantitation. Using this method, heme concentration was determined in chick embryo fibroblasts cultured in a medium supplemented with either 7% fetal bovine serum (FBS) or 10% horse serum (HS). In the presence of FBS, cultured cells actively divided and cells contained 34–55 pmol heme/mg protein. In contrast, cultures maintained in HS proliferated at a slower rate and contained 23–25 pmol heme/mg protein. The addition of 40 μM FeSO4 to cultures maintained in the presence of HS stimulated cell proliferation, and the cellular heme concentration increased to 37–51 pmol/ mg protein. These findings suggest that the cessation of growth in the presence of HS may be due to decreased heme content in the cells and that the stimulation of cell growth by iron is mediated by its stimulation of heme synthesis. 相似文献