Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods

A comparison of five immunohistochemical methods for the demonstration of keratins in human ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase--antiperoxidase (PAP), indirect peroxidase, or the avidin--biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.     

Population Dynamics of Meloidogyne incognita and Rotylenchulus reniformis Alone and in Combination,and Their Effects on Sweet Potato

Meloidogyne incognita (Mi) and Rotylenchulus reniformis (Rr) interactions on sweet potato were studied in naturally and artificially infested field plots for 3 years. In a naturally infested field, early season counts of Mi or Rr were positively correlated with later counts of the same nematode, but negative correlations were found between early Mi and subsequent Rr, and early Rr and subsequent Mi counts. In field plots fumigated with methyl bromide and then infested with low levels of Rr, Mi, and Rr + Mi, final population densities of Mi juveniles were reduced by Rr, but Rr was not affected by Mi. In field plots with a high natural population density of Rr, artificial infestation with high levels of Mi in both fumigated and nonfumigated treatments inhibited Rr, while the final Mi juvenile population density was not affected. Results indicate that a competitive interaction exists with each species capable of inhibiting the other and becoming the dominant population. The nematodes had no apparent effect on yield at the inoculum densities used, either alone or mixed. Both nematodes increased cracking of sweet potatoes, but mixed populations did not differ in incidence of cracking from either Rr or Mi alone.     

Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens

The T- and B-cell surface polypeptides detected by an international workshop panel of 100 mouse monoclonal antibodies (M.Ab) were biochemically defined by radioimmunoprecipitation. Eight T-cell-associated molecules and eight B-cell-associated molecules were identified by multiple antibodies in the panel. Clusters of antibodies specific for the same polypeptide were then compared for their reactivity against peripheral blood mononuclear cells (PBMC) from 11 nonhuman primate species. All the major T- and B-cell antigens present in humans were also expressed in some nonhuman primates. M.Ab to the same antigen were found to react with distinct epitope groups that differed in their phylogenetic distribution. Some epitopes were highly conserved, while other epitopes on the same molecule were only expressed in hominoids and were not detected in old world and new world monkeys. Our detailed analysis of the phylogeny of 37 T-cell antigen epitopes on ten different molecules revealed there was no clear correspondence between the number of epitopes shared and evolutionary distance. Rather the data suggest that parallelism with back mutation may be a common mechanism in the evolution of T-cell antigens. The data also show that the tissue distribution of T-cell antigens can differ between primate species; for example, M.Ab to human Tp45 Tla-Qa-like antigens that did not react with human PBMC did react with PBMC from new world monkeys.     

Effects of toxicants on populations: A qualitative approach II. first order kinetics

System level effects exhibited by a population subjected to a chronic or an acute dose of toxicant are the emphasis of this study. A three dimensional model of a toxicant and a population, with state variables (the population biomass, the concentration of toxicant in an organism, and the concentration of toxicant in the environment) coupled by a linear dose-response function, is analyzed analytically. One of the main results presents sufficient conditions, in terms of a system level parameter, for the persistence, and for the extinction, of a population exposed to a chronic dose of toxicant. When depuration and degradation are negligible processes, the effects of toxicant accumulation associated with an acute exposure of a population are analyzed in some detail. Both persistence and extinction are shown to be viable behavior modes of a population in this biochemical setting.     

“Ischaemic” Colitis in Young Adults

Four cases of transient haemorrhagic colitis seen in young adults resemble 10 others in the literature. The 14 cases are distinguished from the classical transient ischaemic colitis syndrome by their youth, the low incidence of stricture formation, and the prevalence of right-sided lesions.     

Starch Gel Electrophoresis of Catalase and Esterase Isoenzymes from Some Rhizobium and Agrobacterium spp.

S ummary : The esterase and catalase isoenzymes of Agrobacterium tumefaciens and A. radiobacter have been compared to those of the fast growing rhizobia using starch gel electrophoresis. An overall similarity was found for the agrobacteria-rhizobia group although the majority of the agrobacteria possessed only one catalase. Those agro-bacteria with 2 catalases gave patterns similar to Rhizobium meliloti and were 3-ketolactose negative. Agrobacterium pseudotsugi isoenzymes, although fitting the general agrobacteria-rhizobia pattern, were not identical to any specific isogram. The taxonomic value of these findings is discussed.