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The molecular mechanisms by which meiotic events are regulated are at present unknown. To approach this problem, we have exploited the natural synchrony of Lilium meiocytes to compare the nuclear protein profiles of a variety of stages of meiosis. This approach has facilitated the identification of a number of nuclear proteins that appear and disappear in a stagespecific fashion. Here we report the presence of an abundant nuclear protein that first appears during premeiotic interphase, a period during which the irreversible commitment to meiosis occurs. Antibodies directed against this protein demonstrate its meiosis specificity as well as conservation of the epitope(s) in both mono-and dicotyledonous plant species. Chromatin fractionation studies indicate that this protein, which we have termed meiotin-1, is associated with strings of nucleosomes. Implications for meiotic chromatin packaging and chromosome structure are discussed.by J.H. Taylor 相似文献
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Linear finite-element models (FEMs) have enjoyed an increased use in orthopaedic research, including the use for modeling external fixation devices. These fixator FEMs depend on a number of basic assumptions concerning the overall fixation frame stability and the components' rigidity. Among the more important ones are: (i) rigid fixation at both ends of the pin and sidebar; (ii) that the sidebar can be treated essentially as a rigid entity, with all bending occurring in the bone pins; and (iii) that the system can be treated as linearly elastic. Prior work done by the authors questions some of these assumptions. Thus, this study sought an empirical evaluation of the validity of some of these a priori assumptions. A Hoffmann single half-frame was tested in its standard form and then according to a stepwise protocol wherein the frame was welded to eliminate any possible points of instability. These tests looked at the stability and rigidity in various modes (axial compression, torsion, and medial-lateral and anterior-posterior four-point bending). The basic assumptions concerning the frame stability, frame rigidity and the frame's response to loads were found to be erroneous. Component failure was common under minimal loads and statistically significant differences (p less than 0.05) of up to 75% were noted in frame rigidity among the various frame forms tested. Thus, considerable caution must be exercised when employing the FEM technique for evaluating the fixator properties. 相似文献
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B L Finlay 《Journal of neurobiology》1992,23(9):1159-1171
Regional variations in cell death are ubiquitous in the nervous system. In the retina, cell death in retinal ganglion cells is elevated in the retinal periphery and may be important in setting up the initial conditions that produce central retinal specializations such as an area centralis or visual streak. In central visual system structures, pronounced spatial and spatiotemporal inhomogeneities in cell death are seen both in layers and regions of the lateral geniculate nucleus and superior colliculus; similar indications of inhomogeneities are seen in those nonvisual structures that have been examined. Cell death in the cortex is highly nonuniform, by layer and by cortical area. A variety of possible functions for these regional losses are proposed, in the context of a uniform mechanism for cell death that allows it to assume multiple functions. 相似文献
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A 569 bp probe against the β-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6kb RNA occurred in human adult and normal rat liver. 70% hepatectomy increased expression, peaking at 10 h and returning to near normal levels 24 h after resection. The 6 kb band was strongly expressed in human foetal liver, as compared with adult, but not in foetal kidney or pancreas, suggesting a major role for hepatotropin in both foetal development and regeneration of the liver. 相似文献
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Four patients developed massive pulmonary embolism after jejuno-ileal bypass for morbid obesity. All patients were in Greenfield's Class IV and were in shock. Severe hypoxia was evidenced in their blood gases. The patients were managed with digitalis, diuretics, Solu-Medrol (methylprednisolone sodium succinate), oxygen, and heparin therapy. Each patient underwent partial vena cava interruption with Mobin Uddin's umbrella, and all four survived without residual complications. 相似文献