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991.
Clara Lord Fabien Morat Raymonde Lecomte-Finiger Philippe Keith 《Environmental Biology of Fishes》2012,93(2):209-222
Otolith shape analysis has been used in a number of studies as an inexpensive and powerful method for categorising fish in
individual stocks. Elliptical Fourier analysis was used on three different amphidromous Sicyopterus species. Sicyopterus lagocephalus is a widespread species while the other two have a limited distribution area, Sicyopterus aiensis being endemic to Vanuatu, and Sicyopterus sarasini to New Caledonia. Both endemics live in sympatry with the widespread species. The otolith shape of all fish sampled was a
clear species differentiator, thereby demonstrating that otolith shape is species-specific. At an intraspecific level there
are different river populations within samples from Vanuatu, indicating a western group and an eastern “central” group.These
results are congruent both for the endemic species, S. aiensis and for the cosmopolitan species. Finally, we found that, for
S. lagocephalus, the cosmopolitan species, New Caledonian samples are close to western Vanuatu samples, the latter two being well differentiated
from the eastern “central” Vanuatu samples. The explanation for these results may lay either in the influence of environmental
factors on the otolith shape, or in the influence of common early life history thus reflecting genetic factors, or a combination
of both. 相似文献
992.
Telomeres protect the natural ends of chromosomes from being repaired as deleterious DNA breaks. In fission yeast, absence of Taz1 (homologue of human TRF1 and TRF2) renders telomeres vulnerable to DNA repair. During the G1 phase, when non‐homologous end joining (NHEJ) is upregulated, taz1Δ cells undergo telomere fusions with consequent loss of viability. Here, we show that disruption of the fission yeast MRN (Rad23MRE11‐Rad50‐Nbs1) complex prevents NHEJ at telomeres and, as a result, rescues taz1Δ lethality in G1. Neither Tel1ATM activation nor 5′‐end resection was required for telomere fusion. Nuclease activity of Rad32MRE11 was also dispensable for NHEJ. Mutants unable to coordinate metal ions required for nuclease activity were proficient in NHEJ repair. In contrast, Rad32MRE11 mutations that affect binding and/or positioning of DNA ends leaving the nuclease function largely unaffected also impaired NHEJ at telomeres and restored the viability of taz1Δ in G1. Consistently, MRN structural integrity but not nuclease function is also required for NHEJ of independent DNA ends in a novel split‐molecule plasmid assay. Thus, MRN acts to tether unlinked DNA ends, allowing for efficient NHEJ. 相似文献
993.
Joana M. Jordão Paulo J. Fonseca Maria Clara P. Amorim 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(9):885-895
Choruses have been described mostly in birds, anurans and insects but have been poorly studied in fish. Research in batrachoidid (toadfishes) species suggest vocal facilitation among neighbouring males, but whether chorusing fish present more complex interactions is unknown. In this study, we test the hypothesis that chorusing fish males compete actively to increase attractiveness to females. We first describe vocal interactions in natural choruses of Lusitanian toadfish males. Our analysis found positive correlations between the calling rates of neighbouring males in several occasions. However, we also found that males that showed an overall low vocal activity throughout the observation period exhibited peaks of increased calling activity when neighbours decreased their calling rate, suggesting an opportunistic maximisation of attractiveness. We further test with playback experiments how toadfish males adjust calling activity relative to their neighbours'. We observed that males silent at the time of the playbacks but who had an overall high vocal performance tended to start calling when exposed to playbacks in contrast to low‐activity males. Playback experiments further showed that males initially calling at a high rate adjust their calling rate according to the neighbour's vocal activity level, that is, they increased calling rate when exposed to a high calling rate and decreased it when confronted with a low calling rate. However, males calling at a low rate did not significantly alter their calling rate when presented with a low (similar) or higher calling rate, probably due to temporary physiological and/or ecological constraints. We argue that Lusitanian toadfish males tend to optimise calling effort in relation to their neighbours when they are actively advertising. Further studies are necessary to better understand vocal behaviour with increased chorus size. 相似文献
994.
Diabetes mellitus is an epidemic multisystemic chronic disease that frequently is complicated by complex wound infections. Innovative topical antimicrobial therapy agents are potentially useful for multimodal treatment of these infections. However, an appropriately standardized in vivo model is currently not available to facilitate the screening of these emerging products and their effect on wound healing. To develop such a model, we analyzed, tested, and modified published models of wound healing. We optimized various aspects of the model, including animal species, diabetes induction method, hair removal technique, splint and dressing methods, the control of unintentional bacterial infection, sampling methods for the evaluation of bacterial burden, and aspects of the microscopic and macroscopic assessment of wound healing, all while taking into consideration animal welfare and the '3Rs' principle. We thus developed a new wound infection model in rats that is optimized for testing topical antimicrobial therapy agents. This model accurately reproduces the pathophysiology of infected diabetic wound healing and includes the current standard treatment (that is, debridement). The numerous benefits of this model include the ready availability of necessary materials, simple techniques, high reproducibility, and practicality for experiments with large sample sizes. Furthermore, given its similarities to infected-wound healing and treatment in humans, our new model can serve as a valid alternative for applied research. 相似文献
995.
996.
The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-meiotic S phase (meiS) and the assembly of meiotic chromosome axes, remain poorly defined. We have used genome-wide approaches in Saccharomyces cerevisiae to measure the kinetics of pre-meiotic DNA replication and to investigate the interdependencies between replication and axis formation. We found that replication initiation was delayed for a large number of origins in meiS compared to mitosis and that meiotic cells were far more sensitive to replication inhibition, most likely due to the starvation conditions required for meiotic induction. Moreover, replication initiation was delayed even in the absence of chromosome axes, indicating replication timing is independent of the process of axis assembly. Finally, we found that cells were able to install axis components and initiate recombination on unreplicated DNA. Thus, although pre-meiotic DNA replication and meiotic chromosome axis formation occur concurrently, they are not strictly coupled. The functional separation of these processes reveals a modular method of building meiotic chromosomes and predicts that any crosstalk between these modules must occur through superimposed regulatory mechanisms. 相似文献
997.
Cancer is a devastating disease with a profound impact on society. In recent years, yeast has provided a valuable contribution with respect to uncovering the molecular mechanisms underlying this disease, allowing the identification of new targets and novel therapeutic opportunities. Indeed, several attributes make yeast an ideal model system for the study of human diseases. It combines a high level of conservation between its cellular processes and those of mammalian cells, with advantages such as a short generation time, ease of genetic manipulation and a wealth of experimental tools for genome- and proteome-wide analyses. Additionally, the heterologous expression of disease-causing proteins in yeast has been successfully used to gain an understanding of the functions of these proteins and also to provide clues about the mechanisms of disease progression. Yeast research performed in recent years has demonstrated the tremendous potential of this model system, especially with the validation of findings obtained with yeast in more physiologically relevant models. The present review covers the major aspects of the most recent developments in the yeast research area with respect to cancer. It summarizes our current knowledge on yeast as a cellular model for investigating the molecular mechanisms of action of the major cancer-related proteins that, even without yeast orthologues, still recapitulate in yeast some of the key aspects of this cellular pathology. Moreover, the most recent contributions of yeast genetics and high-throughput screening technologies that aim to identify some of the potential causes underpinning this disorder, as well as discover new therapeutic agents, are discussed. 相似文献
998.
A new species of Euragallia Oman, 1938 from Peru (Pasco Department) is described and illustrated. Euragallia batmanisp. n. can be distinguished from the other species of the genus by the very posteriorly pronounced male pygofer, with an apical hook-like projection, and by the well-developed dorsal area of the aedeagal base, resembling the open wings of a bat. With the addition of Euragallia batmani sp. n., the number of Euragallia species is increased to 21. Only one additional species of the genus is recorded from Peru (Euragallia prion Kramer, 1976). A comparison between the new species and Euragallia prion is provided. A conspicuous structure, which connects the subgenital plates to the styles, is described in detail and named. 相似文献
999.
1000.
David de la Lama-Calvente María José Fernández-Rodríguez Jacinto Gandullo Irene Desena Clara de la Osa Ana Belén Feria Antonia Jiménez-Rodríguez Rafael Borja 《Global Change Biology Bioenergy》2023,15(3):332-345
To reduce the impact on the environment and enhance the sustainability of resources, it is necessary to promote and strengthen the use of landrace cultivars that advocate regenerative agriculture. In this study, the growth and development as well as the anaerobic digestion (AD) of six different landrace cultivars, two commercial hybrids cultivars and a public genotype of Sorghum bicolor have been evaluated. The landrace cultivars, in general, presented greater heights, biomass yields and compactness shoots as well as similar or an improvement in grain production compare to the commercial varieties. The AD of the different sorghum straws was performed in batch mode at mesophilic temperature (35°C). The landrace cultivar Zahina (ZH) obtained the highest final methane yield (413 ± 79 NL CH4 kg−1 VS, volatile solids) but the landrace cultivars Zahina gigante (ZHG) and Trigomillo (TG) were the ones that obtained the highest methane per biomass production (13.7 and 12.7 NL CH4 shoot unit−1, respectively). By contrast, the commercial varieties were the ones that obtained the lowest methane yields. Two mathematical models, first-order kinetics and the Transference Function model, were used to fit the experimental data with the aim of describing and simulating the anaerobic biodegradation of these S. bicolor straw varieties and obtaining the kinetic constants. Both models allowed for adequately fitting the experimental results of methane production with time. In particular, the fastest biomethanization occurred using the commercial variety PR88Y20 (PR88) (specific rate constant k = 0.148 ± 0.008 days−1), while the slowest one was obtained from Panizo (PAN) variety (k = 0.064 ± 0.005 days−1). In addition, the highest values of the maximum methane production rate, Rm, were attained for the varieties ZH and PR88, which were 87.1% and 71.3% higher than that achieved for the PAN variety, which exhibited the lowest value. 相似文献