The purification of 3,3-dimethylallyl- and geranyl-transferase and of isopentenyl pyrophosphate isomerase from pig liver

The enzyme catalysing the synthesis of farnesyl pyrophosphate from dimethylallyl pyrophosphate and isopentenyl pyrophosphate, or from geranyl pyrophosphate and isopentenyl pyrophosphate, has been purified 100-fold from homogenates of pig liver. The enzyme has optimum pH 7.9 and requires Mg(2+) as activator in preference to Mn(2+); it is inhibited by iodoacetamide, N-ethylmaleimide, p-hydroxymercuribenzoate and phosphate ions in addition to the products of the reaction, inorganic pyrophosphate and farnesyl pyrophosphate. From product-inhibition studies of the geranyltransferase reaction, the order of addition of substrates to and release of products from the enzyme has been deduced: geranyl pyrophosphate combines with the enzyme first, followed by isopentenyl pyrophosphate. Farnesyl pyrophosphate dissociates from the enzyme before inorganic pyrophosphate. The existence of isopentenyl pyrophosphate isomerase in liver is confirmed. Methods for the preparation of the pyrophosphate esters of isopentenol, 3,3-dimethylallyl alcohol, geraniol and farnesol are also described.     

The influence of stimulating doses of 6-benzylaminopurine on awakening apple buds and on their consumption of oxygen

Byl sledován vliv 6-benzylaminopurinu na dýchání pupen? jabloně odr?dy Boskooaké v pr?běhu 5 dn? po pod7#x00E1;ní kininu. Bylo zji?těno, ?e spot?eba kyslíku stanovovaná p?ímou metodou Warburgovou je vy??í ji? prvního dne po podání 6-benzylaminopurmu a stoupá stále výrazněji a? do pátého dne, kdy je spot?eba kyslíku u pupen? stimulovaných o 75% vy??í, ne? u pupen? kontrolních.     

New Methods of Chromatographic Separation of Gibberellins A1 and A3

Biologia Plantarum - New Chromatographic methods, chromatography in centrifugal field and thin-layer chromatography on alumina, were used for separating physiologically active gibberellins A1 and...     

Four new species of yeast isolated from insect frass in bark ofTsuga heterophylla (Raf.) Sargent

Summary In a study of the yeasts associated with insect frass underneath the bark ofTsuga heterophylla (the Pacific Coast hemlock) four new species of yeast were found. These were described asSporobolomyces singularis, Bullera tsugae, Cryptococcus skinneri andCandida oregonensis. Sporobolomyces singularis is a non-pigmented species, which required an amendment of the genus definition. Ballistospore formation of the new species ofSporobolomyces and ofBullera was absent on malt agar and on potato glucose agar, but positive on corn meal agar. An unusual case of quantitative transgalactosylation by growing cells ofSporobolomyces singularis on lactose has been described. It has been proposed to establish a “Candida parapsilosis Group” of species to whichC. oregonensis was assigned. Supported by a fellowship of the Calouste Gulbenkian Foundation, Lisbon, Portugal.     

Stability of kappa phage (Serratia marcescens) and its c-mutant at different pH

Устойчивость каппа-фага (Serratia marcescens) и его мутанта «С» исследовалась в буферных растворах при pH от 2.5 до 12 с целью подтверждения, или опровержения, возможности их инактивирования в результате кратковременного доведения кислотности основной суспензии в течение приготовления концентрата фага до pH 4. Было установлено, что каппа-фаг и его с-мутант в среде с pH 4 и выше остаются относительно устойчивыми и что в этих пределах pH между обоими фагами нет существенных различий. Однако при низких pH (2,5–3,5) титр обоих фагов вскоре довольно резко падает, причем с-мутантинакмпбпруемся бысмрее, чем каппа-фаг.     

Synchronization of cell division ofChlorella pyrenoidosa

A modification of the synchronization method forChlorella pyrenoidosa was suggested, based on the mechanical separation of small and large cells by differential centrifugation and dilution of the population after division to a constant density (maximally 3×10⁶ cells/ml.). Using this system, with a 16-hour day and eight hours' darkness, the ontogenetic cycle ofChlorella pyrenoidosa takes 24 hours and synchronous growth and development can be maintained for eight generations.     

Ultrastructure of the surface of multiple scars inSaccharomycodes ludwigii

The use of induced primuline fluorescence led to the discovery of a new type of yeast scars (multiple scars) in the generaKloeckera, Saccharomycodes, Nadsonia andHanseniaspora. The structure and ultrastructure of their surface was studied by electron microscopy, using carbon replicas and isolated cell walls.