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排序方式: 共有111条查询结果,搜索用时 15 毫秒
101.
Giovanni Bacci Patrizia Paganin Loredana Lopez Chiara Vanni Claudia Dalmastri Cristina Cantale Loretta Daddiego Gaetano Perrotta Daniela Dolce Patrizia Morelli Vanessa Tuccio Alessandra De Alessandri Ersilia Vita Fiscarelli Giovanni Taccetti Vincenzina Lucidi Annamaria Bevivino Alessio Mengoni 《PloS one》2016,11(8)
102.
Davide Barreca Giuseppina Laganà Silvana Ficarra Ester Tellone Ugo Leuzzi Salvatore Magazù Antonio Galtieri Ersilia Bellocco 《Biophysical chemistry》2010
During our experimental work, aggregation of bovine serum albumin was obtained incubating the protein solution at 60 °C to investigate temperature-induced secondary structure, conformation changes and anti-aggregative activity of trehalose. IR-measurements suggested that in the presence of 1.0 M of trehalose there is a little increase in short segment connecting ?α-helical and a clearly decrease in the loss of ?α-helix structure and in the formation of intermolecular and antiparellel β-sheet up to 78 and 55%, respectively. Useful information also arose following the temperature evolution of Amide I′ band profile in the range of temperature between 25 and 90 °C in absence or in presence of 1.0 M trehalose. Complementary information is obtained by electrophoresis, circular dichroism, fluorescence spectroscopy, titration of SH groups and light scattering measurements. Results encouraged biotechnology and pharmaceutical application of the disaccharide and provided evidence for its utilization in degenerative diseases evolving via aggregation process. 相似文献
103.
104.
Davide Barreca Giuseppina Laganà Ester Tellone Silvana Ficarra Ugo Leuzzi Antonio Galtieri Ersilia Bellocco 《The Journal of membrane biology》2009,230(3):163-171
The antioxidative activity of some natural flavonoids was analyzed against the stable free radical 2,2-diphenyl-1-picryhydrazyl.
The results indicate that the scavenging power of the tested flavonols is higher than that of the synthetic antioxidants butylated
hydroxyanisole and butylated hydroxytoluene; instead, the flavanones show little activity, as indicated by efficient concentration
(EC50) values. Flavonoid autoxidation and interaction with Fe2+ and hydrogen peroxide were tested using erythrocyte membranes as a model. The results show that some compounds, like hesperetin,
evidence a pro-oxidant activity higher than the ascorbic acid/iron reference system. The compounds with strong oxidative capability
do not only influence cellular redox balance but also activate caspase-3, producing lactate dehydrogenase release and enhancing
anionic exchange at the level of band 3 protein. 相似文献
105.
Calabresel Lilia Capuozzo Elisabetta Galtieri Antonio Bellocco Ersilia 《Molecular and cellular biochemistry》1983,51(2):129-132
Molecular and Cellular Biochemistry - Ceruloplasmin has been isolated from sheep plasma by a procedure involving two chromatographic steps and (NH4)2SO4 fractionation. The ovine protein is similar... 相似文献
106.
107.
Blom Allard Cipolletta Chloe Brunsting Arend M. H. Prins Herbert H. T. 《International journal of primatology》2004,25(1):179-196
We monitored the impact of habituation for tourism through changes in gorillas' behavior during the habituation process at Bai Hokou (Dzanga-Ndoki National Park, Central African Republic) from August 1996 to December 1999. From August 1998 onwards we focused on one gorilla group: the Munye. During the habituation process, it became increasingly easier to locate and to remain with the gorillas. Their initial reactions of aggression, fear and vocalization upon contact were replaced increasingly by ignoring us. Curiosity appeared to be an intermediate stage in the process. The way in which contacts with the Munye ended became more subdued over time. Regular daily contact is important in promoting habituation. Likewise, contacting gorillas while they are in a tree or in dense forest provides positive results compared to open habitat. Contacts within 10 m and contacts without forewarning the gorillas of observer presence, e.g., via tongue clacking, should be avoided. As of December 1999, habituation had progressed well; habituation of western gorillas is feasible. However, the gorillas experience negative effects during the habituation process, showing, for example, an increase in daily path length, and reactions of aggression, fear and vocalization upon contact. Impacts diminish over time. Given these and other potentially negative effects, the decision to begin habituation should not be taken lightly. 相似文献
108.
Salbitani Giovanna Cipolletta Sabrina Vona Vincenza Di Martino Catello Carfagna Simona 《Journal of Plant Growth Regulation》2021,40(1):371-378
Journal of Plant Growth Regulation - Despite being an autotrophic organism, Galdieria phlegrea (Galdieriaceae) has the ability to use glycerol thereby switching to heterotrophy in the dark and... 相似文献
109.
110.
Anne Filipe Alexander Chernorudskiy Sandrine Arbogast Ersilia Varone Rocío-Nur Villar-Quiles Diego Pozzer Maryline Moulin Stefano Fumagalli Eva Cabet Swati Dudhal Maria-Grazia De Simoni Raphaël Denis Nathalie Vadrot Corinne Dill Matteo Giovarelli Luke Szweda Clara De Palma Paolo Pinton Carlotta Giorgi Carlo Viscomi Emilio Clementi Sonia Missiroli Simona Boncompagni Ester Zito Ana Ferreiro 《Cell death and differentiation》2021,28(1):123
SEPN1-related myopathy (SEPN1-RM) is a muscle disorder due to mutations of the SEPN1 gene, which is characterized by muscle weakness and fatigue leading to scoliosis and life-threatening respiratory failure. Core lesions, focal areas of mitochondria depletion in skeletal muscle fibers, are the most common histopathological lesion. SEPN1-RM underlying mechanisms and the precise role of SEPN1 in muscle remained incompletely understood, hindering the development of biomarkers and therapies for this untreatable disease. To investigate the pathophysiological pathways in SEPN1-RM, we performed metabolic studies, calcium and ATP measurements, super-resolution and electron microscopy on in vivo and in vitro models of SEPN1 deficiency as well as muscle biopsies from SEPN1-RM patients. Mouse models of SEPN1 deficiency showed marked alterations in mitochondrial physiology and energy metabolism, suggesting that SEPN1 controls mitochondrial bioenergetics. Moreover, we found that SEPN1 was enriched at the mitochondria-associated membranes (MAM), and was needed for calcium transients between ER and mitochondria, as well as for the integrity of ER-mitochondria contacts. Consistently, loss of SEPN1 in patients was associated with alterations in body composition which correlated with the severity of muscle weakness, and with impaired ER-mitochondria contacts and low ATP levels. Our results indicate a role of SEPN1 as a novel MAM protein involved in mitochondrial bioenergetics. They also identify a systemic bioenergetic component in SEPN1-RM and establish mitochondria as a novel therapeutic target. This role of SEPN1 contributes to explain the fatigue and core lesions in skeletal muscle as well as the body composition abnormalities identified as part of the SEPN1-RM phenotype. Finally, these results point out to an unrecognized interplay between mitochondrial bioenergetics and ER homeostasis in skeletal muscle. They could therefore pave the way to the identification of biomarkers and therapeutic drugs for SEPN1-RM and for other disorders in which muscle ER-mitochondria cross-talk are impaired.Subject terms: Chaperones, Respiratory tract diseases 相似文献