全文获取类型
收费全文 | 490篇 |
免费 | 52篇 |
出版年
2023年 | 4篇 |
2022年 | 8篇 |
2021年 | 13篇 |
2020年 | 11篇 |
2019年 | 10篇 |
2018年 | 9篇 |
2017年 | 9篇 |
2016年 | 13篇 |
2015年 | 21篇 |
2014年 | 36篇 |
2013年 | 36篇 |
2012年 | 40篇 |
2011年 | 49篇 |
2010年 | 28篇 |
2009年 | 23篇 |
2008年 | 32篇 |
2007年 | 28篇 |
2006年 | 28篇 |
2005年 | 22篇 |
2004年 | 20篇 |
2003年 | 24篇 |
2002年 | 17篇 |
2001年 | 8篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 7篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1975年 | 1篇 |
1973年 | 2篇 |
排序方式: 共有542条查询结果,搜索用时 15 毫秒
101.
102.
103.
Y Ben Ami K von der Mark A Franzen B De Bernard G C Lunazzi M Silbermann 《The American journal of anatomy》1991,190(2):157-166
This study provides data concerning the cells and their extracellular matrix in prenatal human mandibular condylar cartilage. The latter cartilage represents a secondary type of cartilage since it develops late in the morphogenesis of the craniofacial skeleton. The cartilage of the mandibular condyle is actively involved in endochondral ossification, thus showing all the phases of cartilage growth, maturation, and mineralization that precedes de novo bone formation. The present study focused on the localization and distribution of the major macromolecules that are normally encountered in cartilage and bone, including collagens, proteoglycans, fibronectin, osteonectin, osteocalcin, alkaline phosphatase, and anchorin CII. It became clear that the mineralized zone of the cartilage already contained bone-specific antigens; thus the above zone might serve as an essential propagative predecessor in the ossification process. 相似文献
104.
105.
106.
107.
Ami Matsumoto Yuji Uehara Yoshihiro Shimizu Takuya Ueda Toshio Uchiumi Kosuke Ito 《Proteins》2019,87(3):226-235
Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, which are the products of defective translation, to recycle the tRNA for further rounds of protein synthesis. Pth is ubiquitous in nature, and its activity is essential for bacterial viability. Here, we have determined the crystal structure of Pth from Thermus thermophilus (TtPth) at 1.00 Å resolution. This is the first structure of a Pth from a thermophilic bacterium and the highest resolution Pth structure reported so far. The present atomic resolution data enabled the calculation of anisotropic displacement parameters for all atoms, which revealed the directionality of the fluctuations of key regions for the substrate recognition. Comparisons between TtPth and mesophilic bacterial Pths revealed that their structures are similar overall. However, the structures of the N- and C-terminal, loop-helix α4, and helix α6 regions are different. In addition, the helix α1 to strand β4 region of TtPth is remarkably different from those of the mesophilic bacterial Pths, because this region is 9 or 10 amino acid residues shorter than those of the mesophilic bacterial Pths. This shortening seems to contribute to the thermostability of TtPth. To further understand the determinants for the thermostability of TtPth, we compared various structural factors of TtPth with those of mesophilic bacterial Pths. The data suggest that the decreases in accessible surface area and thermolabile amino acid residues, and the increases in ion pairs, hydrogen bonds, and proline residues cooperatively contribute to the thermostability of TtPth. 相似文献
108.
Thomaz Pinotti Anders Bergström Maria Geppert Matt Bawn Dominique Ohasi Wentao Shi Daniela R. Lacerda Arne Solli Jakob Norstedt Kate Reed Kim Dawtry Fabricio González-Andrade Cesar Paz-y-Miño Susana Revollo Cinthia Cuellar Marilza S. Jota José E. Santos Qasim Ayub Chris Tyler-Smith 《Current biology : CB》2019,29(1):149-157.e3
109.
Takeuchi K Nagata N Kato SI Ami Y Suzaki Y Suzuki T Sato Y Tsunetsugu-Yokota Y Mori K Van Nguyen N Kimura H Nagata K 《Journal of virology》2012,86(6):3027-3037
A major difference between vaccine and wild-type strains of measles virus (MV) in vitro is the wider cell specificity of vaccine strains, resulting from the receptor usage of the hemagglutinin (H) protein. Wild-type H proteins recognize the signaling lymphocyte activation molecule (SLAM) (CD150), which is expressed on certain cells of the immune system, whereas vaccine H proteins recognize CD46, which is ubiquitously expressed on all nucleated human and monkey cells, in addition to SLAM. To examine the effect of the H protein on the tropism and attenuation of MV, we generated enhanced green fluorescent protein (EGFP)-expressing recombinant wild-type MV strains bearing the Edmonston vaccine H protein (MV-EdH) and compared them to EGFP-expressing wild-type MV strains. In vitro, MV-EdH replicated in SLAM(+) as well as CD46(+) cells, including primary cell cultures from cynomolgus monkey tissues, whereas the wild-type MV replicated only in SLAM(+) cells. However, in macaques, both wild-type MV and MV-EdH strains infected lymphoid and respiratory organs, and widespread infection of MV-EdH was not observed. Flow cytometric analysis indicated that SLAM(+) lymphocyte cells were infected preferentially with both strains. Interestingly, EGFP expression of MV-EdH in tissues and lymphocytes was significantly weaker than that of the wild-type MV. Taken together, these results indicate that the CD46-binding activity of the vaccine H protein is important for determining the cell specificity of MV in vitro but not the tropism in vivo. They also suggest that the vaccine H protein attenuates MV growth in vivo. 相似文献
110.
Kazutaka Murayama Miyuki Kato-Murayama Toshiaki Hosaka Ami Sotokawauchi Shigeyuki Yokoyama Kazunari Arima Mikako Shirouzu 《Journal of molecular biology》2012,423(3):386-396
Cucumisin is a plant serine protease, isolated as an extracellular glycoprotein from the melon fruit Cucumis melo L. var. Prince. Cucumisin is composed of multiple domain modules, including catalytic, protease-associated, and fibronectin‐III-like domains. The crystal structure of cucumisin was determined by the multiwavelength anomalous dispersion method and refined at 2.75 Å resolution. A structural homology search indicated that the catalytic domain of cucumisin shares structural similarity with subtilisin and subtilisin-like fold enzymes. According to the Z-score, the highest structural similarity is with tomato subtilase 3 (SBT3), with an rmsd of 3.5 Å for the entire region. The dimer formation mediated by the protease-associated domain in SBT3 is a distinctive structural characteristic of cucumisin. On the other hand, analytical ultracentrifugation indicated that cucumisin is mainly monomeric in solution. Although the locations of the amino acid residues composing the catalytic triad are well conserved between cucumisin and SBT3, a disulfide bond is uniquely located near the active site of cucumisin. The steric circumstances of the active site with this disulfide bond are distinct from those of SBT3, and it contributes to the substrate preference of cucumisin, especially at the P2 position. Among the plant serine proteases, the thermostability of cucumisin is higher than that of its structural homologue SBT3, as determined by their melting points. A structural comparison between cucumisin and SBT3 revealed that cucumisin possesses less surface area and shortened loop regions. Consequently, the higher thermostability of cucumisin is achieved by its more compact structure. 相似文献