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71.
The pep4 gene encoding proteinase A is involved in dimorphism and pathogenesis of Ustilago maydis 下载免费PDF全文
Cinthia V. Soberanes‐Gutiérrez Margarita Juárez‐Montiel Omar Olguín‐Rodríguez César Hernández‐Rodríguez José Ruiz‐Herrera Lourdes Villa‐Tanaca 《Molecular Plant Pathology》2015,16(8):837-846
Vacuole proteases have important functions in different physiological processes in fungi. Taking this aspect into consideration, and as a continuation of our studies on the analysis of the proteolytic system of Ustilago maydis, a phytopathogenic member of the Basidiomycota, we have analysed the role of the pep4 gene encoding the vacuolar acid proteinase PrA in the pathogenesis and morphogenesis of the fungus. After confirmation of the location of the protease in the vacuole using fluorescent probes, we obtained deletion mutants of the gene in sexually compatible strains of U. maydis (FB1 and FB2), and analysed their phenotypes. It was observed that the yeast to mycelium dimorphic transition induced by a pH change in the medium, or the use of a fatty acid as sole carbon source, was severely reduced in Δpep4 mutants. In addition, the virulence of the mutants in maize seedlings was reduced, as revealed by the lower proportion of plants infected and the reduction in size of the tumours induced by the pathogen, when compared with wild‐type strains. All of these phenotypic alterations were reversed by complementation of the mutant strains with the wild‐type gene. These results provide evidence of the importance of the pep4 gene for the morphogenesis and virulence of U. maydis. 相似文献
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Spanevello RM Mazzanti CM Maldonado PA Zanin R Morsch A Hannel L Mazzanti A Festugatto R Graça D Schmatz R Loro VL Schetinger MR Morsch VM 《Life sciences》2007,80(12):1109-1114
The activities of the enzymes NTPDase (EC 3.6.1.5, apyrase, CD39) and 5'-nucleotidase (EC 3.1.3.5, CD73) were analyzed in platelets from rats submitted to demyelination by ethidium bromide (EB) and treated with interferon beta (IFN-beta). The following groups were studied: I - control (saline), II - (saline and IFN-beta), III - (EB) and IV - (EB and IFN-beta). After 7, 15 and 30 days, the animals (n=7) were sacrificed and the platelets were separated by the method of Lunkes et al. [Lunkes, G., Lunkes D., Morsch, V., Mazzanti, C., Morsch, A., Miron, V., Schetinger, M.R.C., 2004. NTPDase and 5'-nucleotidase in rats alloxan- induced diabetes. Diabetes Research and Clinical Practice 65, 1-6]. NTPDase activity for ATP and ADP substrates was significantly lower in groups II and III after seven days, when compared to control (p<0.001). At fifteen days, ATP hydrolysis was significantly lower in group III and IV and higher in group II (p<0.001), while there was an activation of ADP hydrolysis in group II (p<0.001), when compared with the control. 5'-nucleotidase activity was significantly higher in group IV (p<0.001) after seven days, and lower in the groups III and IV (p<0.001) after fifteen days in relation to the control. No significant differences were observed in NTPDase and 5'-nucleotidase activities after thirty days. In conclusion, our study demonstrated that the hydrolysis of adenine nucleotides is modified in platelets of rats demyelinated and treated with IFN-beta. 相似文献
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Bottari Nathieli B. Pillat Micheli M. Schetinger Maria R.C. Reichert Karine P. Machado Vanessa Assmann Charles E. Ulrich Henning Dutra Anielen Morsch Vera M. Vidal Taís Da Cruz Ivana B. M. Melazzo Cinthia Da Silva Aleksandro Schafer 《Purinergic signalling》2019,15(1):77-84
Purinergic Signalling - The effects of Toxoplasma gondii during embryonic development have not been explored despite the predilection of this parasite for neurons and glial cells. Here, we... 相似文献
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Naiara Stefanello Roberta Schmatz Luciane Belmonte Pereira Maribel A. Rubin João Batista Teixeira da Rocha Graziela Facco Maria Ester Pereira Cinthia Melazzo de Andrade Mazzanti Sabina Passamonti Marília Valvassori Rodrigues Fabiano Barbosa Carvalho Michelle Melgarejo da Rosa Jessie Martins Gutierres Andréia Machado Cardoso Vera Maria Morsch Maria Rosa Chitolina Schetinger 《Molecular and cellular biochemistry》2014,385(1-2):277-286
Mesenchymal stem cells (MSCs) represent a potential therapeutic target for glioma. We determined the molecular mechanism of inhibitory effect of human umbilical cord-derived MSCs (hUC-MSCs) on the growth of C6 glioma cells. We demonstrated that hUC-MSCs inhibited C6 cell growth and modulated the cell cycle to G0/G1 phase. The expression of β-catenin and c-Myc was downregulated in C6 cells by conditioned media from hUC-MSCs, and the levels of secreted DKK1 were positively correlated with concentrations of hUCMSCs-CM. The inhibitory effect of hUC-MSCs on C6 cell proliferation was enhanced as the concentration of DKK1 in hUCMSCs-CM increased. When DKK1 was neutralized by anti-DKK1 antibody, the inhibitory effect of hUC-MSCs on C6 cells was attenuated. Furthermore, we found that conditioned media from hUC-MSCs transfection with siRNA targeting DKK1 mRNA or pEGFPN1-DKK1 plasmid lost or enhanced the abilities to regulate the Wnt signaling in C6 cells. Therefore, hUC-MSCs inhibited C6 glioma cell growth via secreting DKK1, an inhibitor of Wnt pathway, may represent a novel therapeutic strategy for malignant glioma. 相似文献
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Fabiana G. Moreira-Gasparin Cristina G. Marques de Souza Andréa M. Costa Ana Maria Alexandrino Cissa K. Bracht Cinthia G. Boer Rosane M. Peralta 《Biodegradation》2009,20(5):727-736
The purpose of this work was to characterize an alkaline protease from the filamentous fungus Myrothecium verrucaria and to explore its capability to degrade native poultry feathers. The enzyme was purified to homogeneity using a single chromatographic
step. Recovery was high, 62%, with a specific activity of 12,851.8 U/mg protein. The enzyme is a small monomeric protein with
a molecular mass of 22 ± 1.5 kDa. It presented pH optimum of 8.3 and was stable over a broad pH range (5.0–12.0). The temperature
optimum was 37°C, with thermal stability at temperatures up to 45°C. The enzyme presented an efficiency of 80.3% in the degradation
of poultry feather meal, releasing amino acids and soluble peptides. It was able to hydrolyze β-keratin without necessity of chemical or enzymatic reduction of the disulphide bonds. Considering that, everyday, poultry-processing
plants produce feathers as a waste products, this protease can be useful in biotechnological processes aiming to improve the
transformation of poultry feathers through solubilization of β-keratin into usable peptides. Furthermore, it can also be useful in processes aiming to reduce the environmental pollution
caused by the accumulation of feathers. 相似文献
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Luciane Belmonte Pereira Cinthia Melazzo de A. Mazzanti Denise Cargnelutti Liana Ver?nica Rossato Jamile F. Gon?alves Nic??ia Calgaroto Valderi Dressler Fernando T. Nicoloso Luiz Carlos Federizzi Vera M. Morsch Maria R. C. Schetinger 《Biometals》2011,24(1):73-83
The phytotoxic effects of aluminum and the mechanisms of genetically-based Al tolerance have been widely investigated, as reported in many papers and reviews. However, investigations on many Al-sensitive and Al-resistant species demonstrate that Al phytotoxicity and Al-resistance mechanisms are extremely complex phenomena. The objective of the present study was to analyze the effects of aluminum on the activity of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Also was evaluated the lipid peroxidation, H2O2 content, levels of ascorbic acid (ASA), non-protein thiols (NPSH) and Al content in three genotypes of oat, Avena sativa L. (UFRGS 930598, UFRGS 17, and UFRGS 280). The genotypes were grown in different concentrations of Al ranging from 90 to 555???M for 5?days. The antioxidant system was unable to overcome toxicity resulting in negative effects such as lipid peroxidation and H2O2 content in UFRGS 930598. The results showed that UFRGS 930598 was the most sensitive genotype. UFRGS 17 and UFRGS 280 were more resistant to Al toxicity. These results suggest that UFRGS 17 has mechanisms of external detoxification and UFRGS 280 has mechanisms of internal detoxification. The different behavior of enzymatic and non-enzymatic antioxidants of the genotypes showed that aluminum resistance in UFGRS 17 and UFRGS 280 may be related to oxidative stress. 相似文献