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排序方式: 共有227条查询结果,搜索用时 9 毫秒
71.
Shams Tabrez Fazlur Rahman Rahat Ali Abdulaziz
S. Alouffi Sajjadul
Kadir Akand Bader
Mohammed Alshehri Fahdah
Ayed Alshammari Aftab Alam Mohammed
A. Alaidarous Saeed Banawas Abdul Aziz
Bin Dukhyil Abdur Rub 《Bioscience reports》2021,41(1)
Cynaroside, a flavonoid, has been shown to have antibacterial, antifungal and anticancer activities. Here, we evaluated its antileishmanial properties and its mechanism of action through different in silico and in vitro assays. Cynaroside exhibited antileishmanial activity in time- and dose-dependent manner with 50% of inhibitory concentration (IC50) value of 49.49 ± 3.515 µM in vitro. It inhibited the growth of parasite significantly at only 20 µM concentration when used in combination with miltefosine, a standard drug which has very high toxicity. It also inhibited the intra-macrophagic parasite significantly at low doses when used in combination with miltefosine. It showed less toxicity than the existing antileishmanial drug, miltefosine at similar doses. Propidium iodide staining showed that cynaroside inhibited the parasites in G0/G1 phase of cell cycle. 2,7-dichloro dihydro fluorescein diacetate (H2DCFDA) staining showed cynaroside induced antileishmanial activity through reactive oxygen species (ROS) generation in parasites. Molecular-docking studies with key drug targets of Leishmania donovani showed significant inhibition. Out of these targets, cynaroside showed strongest affinity with uridine diphosphate (UDP)-galactopyranose mutase with −10.4 kcal/mol which was further validated by molecular dynamics (MD) simulation. The bioactivity, ADMET (absorption, distribution, metabolism, excretion and toxicity) properties, Organisation for Economic Co-operation and Development (OECD) chemical classification and toxicity risk prediction showed cynaroside as an enzyme inhibitor having sufficient solubility and non-toxic properties. In conclusion, cynaroside may be used alone or in combination with existing drug, miltefosine to control leishmaniasis with less cytotoxicity. 相似文献
72.
Muhamad Aidilfitri Mohamad Roslan Nur Qaiyyum Ummi Aiman Jefri Nurhidayah Ramlee Nor Aini Abdul Rahman Nur Hazlin Hazrin Chong Hamidun Bunawan Izwan Bharudin Mohd. Hafiz Abd. Kadir Masita Mohammad Halim Razali 《Saudi Journal of Biological Sciences》2021,28(5):3001-3012
Food waste (FW) minimization at the source by using food waste biodigester (FWBs) has a vast potential to lower down the impact of increasing organic fraction in municipal solid waste generation. To this end, this research sought to check the performance of locally isolated hydrolase-producing bacteria (HPB) to improve food waste biodegradation rate. Two under-explored HPB identified as Bacillus paralicheniformis GRA2 and Bacillus velezensis TAP5 were able to produce maximum amylase, cellulase, protease and lipase activities, and demonstrated a significant hydrolase synergy in co-culture fermentation. In vitro biodegradation analysis of both autoclaved and non-autoclaved FW revealed that the HPB inoculation was effective to degrade total solids (>62%), protein (>19%), total fat (>51), total sugar (>86%), reducing sugar (>38%) and starch (>50%) after 8-day incubation. All co-culture treatments were recorded superior to the respective monocultures and the uninoculated control. The results of FW biodegradation using batch-biodigester trial indicated that the 1500 mL and 1000 mL inoculum size of HPB inoculant reached a plateau on the 4th day, with gross biodegradation percentage (GBP) of >85% as compared to control (66.4%). The 1000 mL inoculum was sufficient to achieve the maximum GBP (>90%) of FW after an 8-day biodigestion in a FWB. 相似文献
73.
In this work, the inhibitory effect of some symmetric sulfamides derived from phenethylamines were determined against human carbonic anhydrase (hCA) I, and II isoenzymes, and compared with standard compound acetazolamide. IC50 values were obtained from the Enzyme activity (%)-[Symmetric sulfamides] graphs. Also, Ki values were calculated from the Lineweaver-Burk graphs. Some symmetric sulfamides compounds ( 11 – 18 ) demonstrated excellent inhibition effects against hCA I, and II isoenzymes. These compounds demonstrated effective inhibitory profiles with IC50 values in ranging from 21.66–28.88 nM against hCA I, 14.44–30.13 nM against hCA II. Among these compounds, the best Ki value for hCA I (Ki: 8.34±1.60 nM) and hCA II (Ki: 16.40±1.00 nM) is compound number 11 . Besides, the IC50 value of acetazolamide used as a standard was determined as hCA I, hCA II 57.75 nM, 49.50 nM, respectively. Moreover, in silico ADME-Tox study showed that all synthesized compounds ( 11 – 18 ) had good oral bioavailability in light of Jorgensen's rule of three, and of Lipinski's rule of five. 相似文献
74.
Rabiatul A. A. R. Fatihhi S. J. Md Saad Amir Putra Zakaria Zulfadzli Harun M. N. Kadir M. R. A. Öchsner Andreas Zaman Tunku Kamarul Syahrom Ardiyansyah 《Biomechanics and modeling in mechanobiology》2021,20(3):957-968
Biomechanics and Modeling in Mechanobiology - The present study has sought to investigate the fluid characteristic and mechanical properties of trabecular bone using fluid–structure... 相似文献
75.
76.
The local geese in the transition region between the Caucasus Mountains and Anatolia have economically significant differences
in productivity and are identified by four feather colors, white, black, piebald, and yellow. This study was undertaken to
determine the genetic structure, evolutionary relationships, and genetic diversity among these birds. DNA samples were obtained
from 100 animals, and 50 random primers were screened. Genetic relationships were determined by random amplified polymorphic
DNA polymorphisms obtained from a total of 48 loci, showing 40 bands (83.33%) that were polymorphic among all the populations
investigated. A dendrogram constructed for this study revealed a close relationship between the white and the black birds.
Additionally, the piebald birds showed close similarity to white and black geese, and the yellow birds displayed a clear distance
from the other three populations. 相似文献
77.
Alireza Valdiani Daryush Talei Arash Javanmard Soon Guan Tan Mihdzar Abdul Kadir Mahmood Maziah 《Gene》2014
Andrographis paniculata Nees. (AP) is a self-pollinated medicinal herb with a wide range of pharmaceutical properties, facing a low diversity in Malaysia. Cross-pollination of AP accessions leads to considerable rates of heterosis in the agro-morphological characteristics and anticancer phytochemicals of this eminent medicinal herb. However, the poor crossability of the plant at the interpopulation or intraspecific levels is an obstacle from the evolutionary and breeding points of view as an average of 4.56% crossability was recorded for AP in this study. Hence, this research aimed to elicit the impact of parental genetic distances (GDs) on the rate of crossability of AP using seven accessions in 21 possible cross combinations. To this end, a set of 55 randomly amplified polymorphic DNA (RAPD) primers and a total of 13 agro-morphological markers were employed to test the hypothesis. Twenty-two out of the 55 RAPD primers amplified a total of 257 bands of which 107 bands were found to be polymorphic. The principal component analysis (PCA) based on the RAPD markers revealed that the studied AP accessions were distributed to three distinct groups. Furthermore, it was noticed that even a minor increase in GD between two parents can cause a decline in their crossability. Unlike, the morphological-based GDs acted neutrally to crossability. This finding suggests that, despite the low genetic diversity among the Malaysian APs, a population prescreening using RAPD markers would be useful to enhance the rate of fruit set through selecting the genetically adjacent parents. 相似文献
78.
Surface plasmons are collective oscillations of free electrons at metallic surfaces. These oscillations can give rise to the intense colors of solutions of plasmon resonance nanoparticles and/or very intense scattering. While the use of plasmonic particle absorption based bioaffinity sensing is now widespread throughout biological research, the use of their scattering properties is relatively ill explored. We refer to the use, utility and control of surface plasmons as plasmonics. In this review and forward-looking article, we discuss the current opinions and uses of plasmonics, as well as speculate on areas of future research. These include the use of plasmon scatter for long-range immunosensing and macromolecular conformation studies, as well as the ability to Stokes shift plasmon scatter, a plasmonics phenomenon recently referred to as metal-enhanced fluorescence. 相似文献
79.
Valdiani A Kadir MA Tan SG Talei D Abdullah MP Nikzad S 《Molecular biology reports》2012,39(5):5409-5424
Nain-e Havandi (Andrographis paniculata Nees.) (AP) is an annual herbaceous plant belonging to the family Acanthacea. Only a few species of Andrographis genus out of 28 are medicinally concerned of which AP is the most important. Knowledge about the arrival of AP to Iran is
extremely lacking but most probably it has been imported from India. However, evidence implies the familiarity of Iran’s folkloric
medicine with this plant, but it has been disappeared from contemporary medicine for unknown reasons. Presence of active ingredients
from diterpenoids group such as andrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide has given
incredible unique medicinal properties to the plant. Traditionally, Nain-e Havandi has been used in the role of a non-farm
plant as a remedy for skin problems, flu, respiratory disease, and snakebite in East and Southeast Asia for centuries. Recently,
it has been utilized as a treatment for HIV, hepatitis, diabetes, cancer and kidney disorders. Intensive cultivation of the
herb started only in the past decade in countries such as China, India, Thailand, Indonesia, West Indies, Mauritius and to
some extent, in Malaysia. Availability of different ecological zones in Iran complies with reestablishment of AP in tropical
and temperate regions of the country. This is killing two birds with one stone, supporting the conservational and economic
aspects. 相似文献
80.