Redefining Rationality: Paranormal Investigators' Humour in England

In contemporary England, amateur paranormal investigators are actively engaged in attempts to produce objective knowledge about the ghostly and paranormal. Their project requires them to balance subjective, personal encounters with objective, technologically mediated ones. In doing so, they struggle to align their project with dominant understandings of rationality. Drawing on an ethnographic study of knowledge production among paranormal investigators, I explore paranormal investigators' use of humour and argue that they rely on humorous performances to align themselves with a powerful, hegemonic notion of rationality. Through their humour, they do not contest the scope of rationality; rather, they locate themselves as central to it.     

Understanding Sign Language Bibles through Affective Audits in South India

This article analyses the role that the emic category of understanding plays in creating new forms of personhood and new worlds for sign language using deaf people in south India. As an ethnographic study of the production, dissemination, and circulation of Indian Sign Language Bible DVDs by an international non-denominational Christian missionary organization, this article analyses how the power of sign language as heart language lies in the potentiality of becoming a fluent signer and a member of a deaf sociality. Bringing the Anthropology of Christianity in conversation with the Anthropology of deafness/sign language studies, this article argues that anthropologists have ignored practices of verifying understanding in our interlocutors. In utilizing the concept of affective audits, this article analyses the practices by which understanding comes to take place. In addition, this article also argues that anthropologists must attend to how research on sensory formations might be presuming a ‘normal’ sensing body.     

Circadian preference and decision-making styles

The present study aimed to explore for the first time the relationship between circadian preference and different decision-making styles. In total, 501 young adults (330 females), with a mean age of 21.07 ± 1.99 years, took part in the study. The participants completed the reduced version of the morningness–eveningness questionnaire (rMEQ) and the general decision-making style inventory (GDMS). The rMEQ enabled to assess the circadian preference, with lower rMEQ scores pointing toward eveningness preference. The GDMS measured five decision-making styles: rational, intuitive, dependent, avoidant and spontaneous. Higher scores on each GDMS decision-making style reflect a higher prevalence of the corresponding style. A set of multiple regression analyses was performed with rMEQ score, gender and age as predictors together with each GDMS decision-making style as dependent variable. rMEQ score proved to be the only significant (negative) predictor of avoidant and spontaneous decision-making styles, i.e. lower rMEQ score (tendency toward eveningness) significantly predicted higher score at these decision-making styles. The present results suggest that eveningness preference is significantly related to avoidant and spontaneous decision-making styles in young adults. Such results will be discussed with reference to the effects of decision-making styles on decision-making in different types of workers and mental health.     

Multiplex single molecule counting technology used to generate interleukin 4, interleukin 6, and interleukin 10 reference limits

Detecting biomarkers at pg/ml concentrations or below is, in many situations, critical for quantifying levels in healthy individuals as well as the changes that can occur in the progression of disease states. The ability to detect multiple biomarkers from the same sample allows for better diagnoses, more efficient testing, and lower volumes of sample required. Based on single molecule counting technology, a multiplex instrument was designed and built that is capable of detecting cytokines and other low-abundance proteins at sub-pg/ml quantities in human plasma samples. The multiplex single molecule counting instrument was used to generate 95% reference limits for interleukin 4 (IL-4, <0.61 pg/ml), interleukin 6 (IL-6, <6.53 pg/ml), and interleukin 10 (IL-10, <1.08 pg/ml) from 100 healthy human donor plasma samples, with more than 90% of IL-4 concentrations and 100% of IL-6 and IL-10 concentrations above the limit of detection.     

The assessment of the relevance of building components and life phases for the environmental profile of nearly zero-energy buildings: life cycle assessment of a multifamily building in Italy

Purpose

Since the construction sector is a considerable energy consumer and greenhouse gas (GHG) producer, the EU rules strive to build nearly zero-energy buildings, by reducing the operative energy and yearning for on-site energy production. This article underlines the necessity to go beyond the energy evaluations and move towards the environmental assessment in a life cycle perspective, by comparing the impacts due to building materials and energy production devices.

Methods

We compared the operational energy impacts and those of technologies and materials carrying out a life cycle assessment (LCA; ISO 14040, ISO 14044, EN 15643–2, EN 15978) on a nearly zero-energy building (ZEB), a residential complex with 61 apartments in four buildings, situated near Milan (Italy). We consider all life cycle phases, including production, transport, building site activities, use and maintenance; the materials inventory was filled out collecting data from invoices paid, building site reports, construction drawings and product data sheets. To make the assessment results comparable, we set a functional unit of 1 m² of net floor area in 1 year (1 m²y), upon a lifespan of 100 years. The environmental data were acquired from Ecoinvent 2.2.

Results and discussion

The results highlight the important role of the pre-use and maintenance phases in building life so that in a nearly ZEB, the environmental impacts linked to the use are no longer the major proportion: the pre-use phase accounts for 56 %, while the operative energy is only 31 % of the total. For this reason, if the environmental assessment of the case study was shrunk to the operational consumption, only one third of the impacts would be considered. The consumption of non-renewable resources after 100 years are 193,950 GJ (133.5 kWh/m²y); the GHG emissions are 15,300 t (37.8 kg of CO₂?eq/m²y). In the pre-use phase, structures have the major impacts (50 %) and the load of system components is unexpectedly high (12 %) due to the ambition of on-site energy production.

Conclusions

Paying attention to the operative energy consumption seems to address to only one third of the environmental impacts of buildings: the adoption of LCA as a tool to guide the design choices could help to identify the solution which ensures the lowest overall impact on the whole life, balancing the options of reducing the energy requirements, the on-site production from renewable sources and the limitation of the impacts due to building components (simpler and more durable).

     

A broad mercury resistant strain of <Emphasis Type="Italic">Pseudomonas putida</Emphasis> secretes pyoverdine under limited iron conditions and high mercury concentrations

The Pseudomonas putida FB1, known as a broad-spectrum mercury resistant strain, becomes yellow-green due to the secretion of pyoverdine (PVDs) under limited iron conditions and high mercury concentrations. Different modified Nelson’s media were obtained by adding mercury, iron, and the complexing agent nitrilotriacetic acid to demonstrate that the strain produces only the highest concentrations of PVDs due to the induction with 25 µM Hg²⁺. An amount of 250 mg PVDs was purified from the supernatant of 1 litre culture. The various forms of PVDs were characterized using different techniques such as fluorescence spectroscopy, high performance liquid chromatography coupled with high resolution mass spectrometry, and scanning electron microscope equipped with energy dispersive X-ray analyser. A set of “in vivo” experiments demonstrated that additions of Hg²⁺ to the cultures from 10 to 25 µM Hg²⁺ stimulate an over secretion of PVDs suggesting that the toxic cation strongly reduces the availability of apo-PVDs, because the complex mercuric-pyoverdine is very stable at neutral pH, and hinder the formation of PVDs-Fe(III).