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71.
Berini Francesca Verce Marko Ausec Luka Rosini Elena Tonin Fabio Pollegioni Loredano Mandić-Mulec Ines 《Applied microbiology and biotechnology》2018,102(5):2425-2439
Applied Microbiology and Biotechnology - Bioinformatics has revealed the presence of putative laccase genes in diverse bacteria, including extremophiles, autotrophs, and, interestingly, anaerobes.... 相似文献
72.
Characterization of the restricted component of Epstein-Barr virus early antigens as a cytoplasmic filamentous protein. 总被引:1,自引:1,他引:0
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Four monoclonal antibodies produced against the restricted component of the Epstein-Barr virus (EBV) early antigen (EA-R) precipitated a polypeptide with an approximate molecular weight of 85,000. Three of these antibodies prepared against the native 85,000-molecular-weight protein (85K protein) reacted by immunofluorescence with acetone-fixed smears but not methanol-fixed smears of EBV-producing cells activated with tumor-promoting agent and sodium butyrate. The fourth monoclonal antibody which was produced against the denatured 85K protein reacted with both acetone-fixed cells and methanol-fixed cells. Blocking of direct immunofluorescence by the different monoclonal antibodies established that these monoclonal antibodies were directed against three different epitopes expressed on the 85K protein. The cytoplasmic staining pattern produced by each antibody was granular during the first 24 to 28 h after induction, developed into filamentous structures about 36 h after induction, and then began to aggregate after 48 h. Similar structures were observed in human placental cells transfected by EBV DNA and stained with three of the monoclonal antibodies. These results suggest that the EA-R polypeptide is assembled into filaments during the EBV lytic cycle. The significance of this in regards to replication has yet to be determined. Biochemical characterization of this major EA-R component did not reveal any major differences in this protein isolated from different cell lines. 相似文献
73.
‘Does my Diet Affect my Perfume?’ Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets
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Sofija Pavković‐Lučić Marina Todosijević Tatjana Savić Vlatka Vajs Jelena Trajković Boban Anđelković Luka Lučić Gordana Krstić Slobodan Makarov Vladimir Tomić Dragana Miličić Ljubodrag Vujisić 《化学与生物多样性》2016,13(2):224-232
Cuticular hydrocarbons (CHCs) in Drosophila melanogaster represent the basis of chemical communication being involved in many important biological functions. The aim of this study was to characterize chemical composition and variation of cuticular profiles in five D. melanogaster strains. These strains were reared for approximately 300 generations on five diets: standard cornmeal medium and substrates prepared with apple, banana, tomato, and carrot. Differences in quantity and/or quality in CHCs were assumed as a result of activation of different metabolic pathways involved in food digestion and adaptations to the particular diet type. In total, independently of sex and strain, 66 chemical compounds were identified. In females of all strains, 60 compounds were identified, while, in males, 47 compounds were extracted. Certain new chemical compounds for D. melanogaster were found. MANOVA confirmed that CHC amounts significantly depend on sex and substrates, as well as on their interactions. Discriminant analysis revealed that flies belonging to ‘apple’ and ‘carrot’ strains exhibited the most noticeable differences in CHC repertoires. A non‐hydrocarbon pheromone, cis‐vaccenyl acetate (cVA) also contributed to the variation in the pheromone bouquet among the strains. Variability detected in CHCs and cVA may be used in the explanation of differences in mating behaviour previously determined in analyzed fly strains. 相似文献
74.
75.
Three human cases having mutations in the glycine N-methyltransferase (GNMT) gene have been reported. This enzyme transfers a methyl group from S-adenosylmethionine (SAM) to glycine to form S-adenosylhomocysteine (SAH) and N-methylglycine (sarcosine) and is believed to be involved in the regulation of methylation. All three cases have mild liver
disease but they seem otherwise unaffected. To study this further, gnmt deficient mice were generated for the first time. This resulted in the complete absence of GNMT protein and its activity
in livers of homozygous mice. Compared to WT animals the absence of GNMT resulted in up to a 7-fold increase of free methionine
and up to a 35-fold increase of SAM. The amount of SAH was significantly decreased (3 fold) in the homozygotes compared to
WT. The ratio of SAM/SAH increased from 3 in WT to 300 in livers of homozygous transgenic mice. This suggests a possible significant
change in methylation in the liver and other organs where GNMT is expressed. 相似文献
76.
77.
Maurizio Gaetani Alda Nicora Charles Henderson Simonetta Cirilli Luka Gale Roberto Rettori Irene Vuolo Viorel Atudorei 《Facies》2013,59(4):915-948
New sampling on critical intervals of the uppermost Permian and Triassic successions of the Northern Karakorum Terrain in the Karakorum Range (Pakistan) has refined the stratigraphy. Two types of successions may be distinguished in the Karakorum Range: a carbonate platform succession, spanning the whole interval from Upper Permian to Upper Triassic, possibly with several gaps; and a basinal succession, deposited from the Middle Permian to Early Carnian (Late Triassic), when the carbonate platform prograded into the basin. With the approaching and later docking of the Karakorum Block against the Asian margin closing the Paleo-Tethys, a portion of Karakorum emerged while another part subsided as a fore-deep, receiving clastics from the emerging Cimmerian Range. Molassic sediments filled the basin, whilst shallow-water carbonates transgressed over the emerged carbonate platform sometime between the latest Triassic and the Pliensbachian (Early Jurassic), with Cimmerian deformation occurring to the north. The age control is provided by conodonts, with assemblages of late Wuchiapingian, Changhsingian, Induan (Griesbachian and Dienerian), late Olenekian, early Anisian, late Ladinian, and early Carnian ages, respectively. Some information on the section around the P/T boundary is provided by palynology and isotopic C13 values. The dating of the Norian/Rhaetian platform is provided by foraminifers. 相似文献
78.
79.
Luka Fajs Xhevat Jakupi Salih Ahmeti Isme Humolli Isuf Dedushaj Tatjana Av?i?-?upanc 《PLoS neglected tropical diseases》2014,8(1)
Crimean-Congo hemorrhagic fever virus (CCHFV) is a zoonotic agent that causes severe, life-threatening disease, with a case fatality rate of 10–50%. It is the most widespread tick-borne virus in the world, with cases reported in Africa, Asia and Eastern Europe. CCHFV is a genetically diverse virus. Its genetic diversity is often correlated to its geographical origin. Genetic variability of CCHFV was determined within few endemic areas, however limited data is available for Kosovo. Furthermore, there is little information about the spatiotemporal genetic changes of CCHFV in endemic areas. Kosovo is an important endemic area for CCHFV. Cases were reported each year and the case-fatality rate is significantly higher compared to nearby regions. In this study, we wanted to examine the genetic variability of CCHFV obtained directly from CCHF-confirmed patients, hospitalized in Kosovo from 1991 to 2013. We sequenced partial S segment CCHFV nucleotide sequences from 89 patients. Our results show that several viral variants are present in Kosovo and that the genetic diversity is high in relation to the studied area. We also show that variants are mostly uniformly distributed throughout Kosovo and that limited evolutionary changes have occurred in 22 years. Our results also suggest the presence of a new distinct lineage within the European CCHF phylogenetic clade. Our study provide the largest number of CCHFV nucleotide sequences from patients in 22 year span in one endemic area. 相似文献
80.
Tsuda Y Caposio P Parkins CJ Botto S Messaoudi I Cicin-Sain L Feldmann H Jarvis MA 《PLoS neglected tropical diseases》2011,5(8):e1275