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991.
Salima Machkour-M’Rabet Yann Hénaut Sophie Calmé Luc Legal 《Journal of Insect Conservation》2012,16(4):479-488
Landscape fragmentation usually has a considerable effect on the genetic and demographic viability of most species because it reduces population size and increases isolation among populations. This situation provokes loss of genetic diversity and increased inbreeding that can lead to population or species extinctions. Some studies also show that landscape fragmentation may have no effect on or even positive consequences for species genetic diversity. The protected tarantula, Brachypelma vagans, exhibits a particular situation in the Mexican Caribbean, which has experienced high lowland and coastal fragmentation because of recent increases in agricultural, urban and touristic development. This modified landscape structure creates favorable conditions for establishment of B. vagans populations in rural settlements. Populations of this tarantula have high densities of individuals, principally females and juveniles, and gene dispersion is assumed by the rare males. Within this context, we studied the influence of natural and anthropogenic fragmentation on the genetic diversity of six B. vagans populations (five continental, one insular), together with their spatial organization. Our approach used seven inter simple sequence repeat markers, which are highly polymorphic markers. The 76 loci selected revealed high genetic variability for continental populations and a low, but not critical situation, for the insular population. We detected a good level of gene exchange among continental populations, and an evident and recent isolation of the island population. This species exhibits a metapopulation structure in the lowlands with numerous local populations where mature females exhibit high birth site fidelity. We conclude that this protected species does not exhibit characteristics to warrant its current conservation status, and we propose complete revision of the ecological and genetic situation for B. vagans in particular, and for all species within the genus Brachypelma in general. 相似文献
992.
S. Nibouche L. M. Raboin J.-Y. Hoarau A. D’Hont L. Costet 《Molecular breeding : new strategies in plant improvement》2012,29(1):129-135
The spotted stem borer (SSB) Chilo sacchariphagus is a major pest of sugarcane, causing substantial losses in cane weight and in sucrose yield. SSB resistance is an important
trait to be taken into account for sugarcane breeding programs. In order to analyse the genetic basis of the resistance to
SSB, we undertook a quantitative trait allele (QTA) mapping study based on a population of 147 progenies derived from the
selfing of the resistant modern cultivar R570. The experimental population was evaluated in a replicated trial for borer damage
under natural infestation in two successive crop cycles. A single-factor analysis using 1,405 polymorphic markers was performed
to detect marker–trait associations. Statistical thresholds based on permutation tests designed to control type I errors at
a low level allowed the detection of nine QTAs whose individual size ranged between 6 and 10% of the total variation. These
nine QTAs are distributed over five of the eight homeology groups of the polyploid R570 genome. Two QTAs were found to co-localize
with two typical resistance gene analog clusters. Overall, eight QTAs explain altogether 42% of the total phenotypic variance. 相似文献
993.
Suvendu Mondal A. M. Badigannavar S. F. D’Souza 《Molecular breeding : new strategies in plant improvement》2012,29(2):467-476
Rust is a serious and the most prevalent groundnut disease in tropical and subtropical growing regions of the world. A total
of 164 recombinant inbred lines derived from resistant (VG 9514) and susceptible (TAG 24) cultivated groundnut parents were
screened for rust resistance in five environments. Subsequent genotyping of these lines with 109 simple sequence repeat (SSR)
markers generated a genetic linkage map with 24 linkage groups. The total length of the linkage map was 882.9 cM with an average
of 9.0 cM between neighbouring markers. The markers pPGPseq4A05 and gi56931710 flanked the rust resistance gene at map distances
of 4.7 cM and 4.3 cM, respectively, in linkage group 2. The significant association of these two markers with the rust reaction
was also confirmed by discriminant analysis. The informative SSR markers classified rust-resistant and susceptible groups
with 99.97% correctness. The SSR markers pPGPseq4A05 and gi56931710 were able to identify all the susceptible genotypes from
a set of 20 cultivated genotypes differing in rust reaction. Tagging of the rust resistance locus with linked SSR markers
will be useful in selecting the rust resistant genotypes from segregating populations and in introgressing the rust resistance
genes from diploid wild species. 相似文献
994.
Significant spatial variation in species composition of microphytobenthos often occurs at scales of decimetres. This microscale variation is typically more connected with dispersal-related events than to environmental factors. In this study, 4 microscale transects were delimited at 4 temperate lowland peat bog localities to investigate spatial and temporal microscale variations in benthic desmids (Desmidiales, Viridiplantae). Significant spatial autocorrelation was detected in most of the transects taken 3 times, in September and December 2010 and March 2011. The relative abundance of species data produced more pronounced spatial patterns than the presence?Cabsence data. Spatial autocorrelation mostly decreased during the winter period, possibly due to meteorological disturbances, resulting in less spatially structured phytobenthic community in the March transects. In most cases, spatial distance accounted for a significant part of the variation in a community structure, even in analyses that controlled for the effects of environmental and temporal factors. This indicated that pure spatial factors should be considered important for structuring phytobenthic communities, even across a temporal time span of 6?months. The reduced data sets that included only 25?% of the most frequented species produced very similar patterns in spatial and temporal autocorrelation as the full data sets. Consequently, we concluded that microscale variation of benthic desmids may be sufficiently represented by dynamics of the common species. 相似文献
995.
A. A. Egorshina R. M. Khairullin A. R. Sakhabutdinova M. A. Luk’yantsev 《Russian Journal of Plant Physiology》2012,59(1):134-140
Wheat (Triticum aestivum L., cv. Kazakhstanskaya 10) seedling growth activation by the endophytic 11BM strain of the bacterium Bacillus subtilis Cohn and the involvement of phytohormones in this process were studied. At the concentration used, bacteria enhanced wheat
root and shoot growth as compared to control. Biotests demonstrated auxin-, cytokinin-, and gibberellin-like activities of
the endophyte, but the result depended on the way of experiment performing. When wheat seeds were treated with the spores
of B. subtilis 11BM strain, the concentrations of IAA and ABA increased transiently in the roots and shoots of the seedlings. The involvement
of IAA oxidase in the plant response to inoculation with bacteria was shown; it was accompanied by a decrease in enzyme activity,
which occurred later than auxin accumulation. It was concluded that observed changes in the hormonal status of wheat plants
under the influence of the endophytic bacterial strain may be one of the mechanisms for seedling growth stimulation. 相似文献
996.
Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) is a photoreceptor specifically for UV-B light that initiates photomorphogenic responses in plants. UV-B exposure causes rapid conversion of UVR8 from dimer to monomer, accumulation in the nucleus, and interaction with CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), which functions with UVR8 in UV-B responses. Studies in yeast and with purified UVR8 implicate several tryptophan amino acids in UV-B photoreception. However, their roles in UV-B responses in plants, and the functional significance of all 14 UVR8 tryptophans, are not known. Here we report the functions of the UVR8 tryptophans in vivo. Three tryptophans in the β-propeller core are important in maintaining structural stability and function of UVR8. However, mutation of three other core tryptophans and four at the dimeric interface has no apparent effect on function in vivo. Mutation of three tryptophans implicated in UV-B photoreception, W233, W285, and W337, impairs photomorphogenic responses to different extents. W285 is essential for UVR8 function in plants, whereas W233 is important but not essential for function, and W337 has a lesser role. Ala mutants of these tryptophans appear monomeric and constitutively bind COP1 in plants, but their responses indicate that monomer formation and COP1 binding are not sufficient for UVR8 function. 相似文献
997.
I. I. Poletaeva O. V. Perepelkina O. S. Boyarshinova I. G. Lil’p N. V. Markina T. B. Timoshenko A. V. Revishchin 《Russian Journal of Developmental Biology》2012,43(6):319-331
Experimental data were reviewed which demonstrated that the neonatal injection effects of certain biologically active drugs (ACTH4?C10 fragment and its analogue Semax, piracetam, caffeine, levetiracetam, busperone, etc.) could be detected in adult animals as changes in physiological and behavioral reactions and in several morphological traits as well. Audiogenic seizures proneness, anxiety-fear and exploration behavior as well as pain sensitivity were analyzed. The remote effects discovered were either similar in direction to those applied to an adult organism, or opposite to it. Pharmacological treatments of such type presumably interfere the CNS development during early postnatal ontogeny and change the normal pattern of brain development. These modulatory influences could be due to changes in neurotransmitter system development and are presumably capable to induce CNS morphological deviations (numbers of neurons, adult neurogenesis). 相似文献
998.
D. V. Yarygin N. O. Min’kova Yu. B. Filippovich 《Russian Journal of Developmental Biology》2012,43(5):264-270
Intracellular localization of serine, cysteine and aspartate proteases, as well as their protein inhibitors, in bombyx grain in the postdiapause period of embryogenesis has been studied. Proteolytic activity of aspartate and cysteine proteases was found in lysosomal, mitochondrial, and nuclear fractions of grains. Serine protease activity was not observed in subcellular fractions of grains of the fourth day of postdiapause development. It has been shown that activities of protein inhibitors and certain peptide hydrolases in subcellular fractions provide consistent functioning and fine regulation of the proteolytic enzyme complex. 相似文献
999.
V. V. Stakheev A. E. Balakirev O. O. Grigor’eva A. G. Shestak S. G. Potapov Yu. M. Borisov V. N. Orlov 《Biology Bulletin》2012,39(10):834-838
Microsatellite and mtDNA markers have been used to identify two pairs of cryptic species Sorex araneus L. (Sorex satunini Ogn.) and Sorex minutus L. (S. volnuchini Ogn.). The new data were obtained on the distribution of these species on the plain between the Don and Kuban rivers. The shrew S. volnuchini and Caucasian mole are distributed on the plain areas up to the Eya River. The endemic species of the Caucasus, the shrews S. satunini and S. volnuchini, are widely distributed across the valleys alongside small streams on the steppe plain. Both species are most abundant on thick grasslands, bushy scrub, and deciduous woodlands. They don??t show any clear differences in habitat preferences. 相似文献
1000.
G. A. Afanas’eva 《Biology Bulletin》2012,39(2):137-151
In this work we discuss the results of ontogenetic and morphobiological approaches to phylogenetic reconstructions of articulate
brachiopods on the basis of morphogenetic data. These data provide the basis for generalizations concerning phylogeny and
system of orders. The ontogenetic approach was the subject of criticism mainly because of arbitrary establishment of the homology
of brachiopods. The constraints of the morphobiological approach are associated with the phenomena of morphological evolution
without adaptive explanation so far. As an example of these phenomena, homeomorphism of brachiopods can be considered. Prospects
for morphogenetic studies of brachiopods can be connected with the study of characteristic elements of their shell structure,
such as porosity and mantle channels. 相似文献