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21.
Zheng-Zhao Liu Chun-Gu Hong Wen-Bao Hu Meng-Lu Chen Ran Duan Hong-Ming Li Tao Yue Jia Cao Zhen-Xing Wang Chun-Yuan Chen Xiong-Ke Hu Ben Wu Hao-Ming Liu Yi-Juan Tan Jiang-Hua Liu Zhong-Wei Luo Yan Zhang Shan-Shan Rao Ming-Jie Luo Hao Yin Yi-Yi Wang Kun Xia Lang Xu Si-Yuan Tang Rong-Gui Hu Hui Xie 《Autophagy》2021,17(10):2766
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Xin Jia-Ying Cui Jun-Ru Zhu Li-Min Chen Jian-Bo Xia Chun-Gu Li Shu-Ben 《World journal of microbiology & biotechnology》2002,18(7):609-614
Methylomonas sp. GYJ3 is a methanotrophic bacterium containing methane monooxygenase (MMO), which catalyses the epoxidation of propene to epoxypropane. In this study, the cell suspension of Methylomonas sp. GYJ3 has been used for epoxypropane biosynthesis from propene. When propene is epoxidized, the product epoxypropane is not further metabolized and accumulates extracellularly. Unfortunately, continuous production of epoxypropane is usually difficult due to exhaustion of reductant and the accumulation of toxic products. Hence, in order to address these problems, batch experiments were performed to explore the possibility of producing epoxypropane by a co-oxidation process. Methane was chosen as the most suitable electron-donating co-substrate since it did not result in molecular toxicity and provided abundant reductant for epoxidation. It was found that the maximum production of epoxypropane occurred in an atmosphere of 30% methane. Batch experiments also indicated that continuous removal of product was necessary to overcome the inhibition of epoxypropane. In continuous experiments, optimum mixed gaseous substrates were continuously circulated through the stirred tank bioreactor to remove product from the cell suspension. Initial epoxypropane productivity was 268 mol/day. The bioreactor has been allowed to operate continuously for 12 days without obvious loss of epoxypropane productivity, and more than 96% of initial MMO activity was retained. 相似文献