排序方式: 共有16条查询结果,搜索用时 0 毫秒
11.
Serebryanyi V. A. Vavilova E. A. Chulkin A. M. Vinetskii Yu. P. 《Applied Biochemistry and Microbiology》2002,38(5):420-426
The complete gene xylA that encodes endo-1,4--xylanase secreted byPenicillium canescens was cloned and sequenced. The coding region of the gene is separated by eight introns. The protein comprises 302 amino acids of the mature protein and 25 amino acids of the signal peptide. The xylanase of P. canescens belongs to the glycosyl hydrolase family 10. Nucleotide sequences for binding catabolite repression protein CREA and transactivator protein were detected in the promoter region. A set of multicopy strains displaying a seven to eightfold increase in xylanase yield was obtained. The fraction of xylanase in most productive strains amounted to 30–50% of the total secreted protein. 相似文献
12.
13.
A. M. Chulkin D. S. Loginov E. A. Vavilova A. R. Abyanova I. N. Zorov S. A. Kurzeev O. V. Koroleva S. V. Benevolensky 《Applied Biochemistry and Microbiology》2009,45(2):143-149
The gene egl3 of the filamentous fungus Penicillium canescens endo-1,4-β-glucanase, belonging to family 12 glycosyl hydrolases, was cloned and sequenced. The gene was expressed in P. canescens under the control of the strong promoter of gene bgaS, coding for β-galactosidase of this fungus, and efficient endoglucanase producer strains were obtained. The recombinant protein was isolated from the culture liquid of the producer strain EGL3-13 and purified to homogeneity; its specific activity was 31.7 IU; molecular weight, 26 kDa; and pH and temperature optimums, 3.2 and 54°C, respectively. The K m and V m values for CMC hydrolysis were determined; they amounted to 17.1 g/l and 0.31 μM/(mg s), respectively. 相似文献
14.
15.
16.