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141.
Area postrema is rich in angiotensin II receptors and intravenous (iv) administration of angiotensin II has been reported to elicit emesis. However, in the present study intracerebroventricular (icv) administration of angiotensin II up to a dose of 10 micrograms failed to elicit emesis. It is suggested that presence of a cerebrospinal fluid-brain barrier in area postrema most probably prevents access of icv angiotensin II to its receptors which are otherwise accessible on iv administration. 相似文献
142.
Chandrasekhar Gurramkonda Sulena Polez Natasa Skoko Ahmad Adnan Thomas Gäbel Dipti Chugh Sathyamangalam Swaminathan Navin Khanna Sergio Tisminetzky Ursula Rinas 《Microbial cell factories》2010,9(1):31
Background
The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries. 相似文献143.
144.
The phenotypes and gene frequencies of 3 blood groups, 7 red-cell enzymes and a serum protein were studied in 4 caste population groups of Haryana, North India. The results indicate that the distribution of these blood markers is rather homogeneous in the 4 groups and generally resembles that observed in various populations from neighbouring North Indian states. 相似文献
145.
A repertoire of monoclonal antibodies (mAbs) was generated against the midgut proteins of Anopheles culicifacies mosquitoes. The mAbs AC-43 and AC-29 significantly inhibited Plasmodium vivax development inside the mosquito midgut. The number of oocysts that developed was reduced by 78.6% when mosquitoes ingested
a combination of these two mAbs along with the blood meal. AC-43 mAb binds to the epitope common in 97, 80 and 43 kDa polypeptides
from the midgut protein extract, as indicated by western blot analysis. Similarly, the mAb AC-29 recognized 52, 44, 40 and
29 kDa polypeptides. These female midgut-specific polypeptides are shared between An. culicifacies and An. stephensi, two major vectors of malaria in India. Deglycosylation assays revealed that O-linked carbohydrates are the major components in epitopes corresponding to AC-43 and AC-29. Gold particle labelling revealed
that both these mAbs preferentially bind to glycoproteins at the apical microvilli and the microvillus-associated network
present inside transverse sections of the gut epithelium. These regions are particularly known to have receptors for ookinetes,
which enable them to cross this epithelial barrier and provide them with certain necessary chemicals or components for further
development into oocysts. Therefore, these glycoproteins appear to be potential candidates for a vector-directed transmission-blocking
vaccine (TBV). 相似文献
146.
Direct gene transformation methods such as microprojectile bombardment have been successfully employed for obtaining transgenics in cereals in general and wheat in particular. As success of any transformation strategy depends largely upon the regeneration capability of the target explant, the present investigation employs leaf basal segments to achieve high regeneration response via somatic embryogenesis. Basal segments of 5-day-old seedlings of T. aestivum var. CPAN1676 and T. dicoccum var. DDK1001 were cultured on callusing medium for 3 weeks at 26 ± 1 °C, discontinuous light followed by a culture period of 15 days at 21 ± 1 °C in continuous light. The calli were then transferred to auxin-free medium for regeneration in discontinuous light at 26 ± 1 °C. Regeneration via somatic embryogenesis was observed within 2 weeks in T. aestivum var. CPAN1676 and T. dicoccum var. DDK1001 (68 and 82%, respectively). This embryogenic calli were employed further to obtain hygromycin resistance by particle bombardment in T. aestivum and T. dicoccum. A transformation efficiency of 8.6, 7.5 and 4.9% was obtained in T. aestivum var. CPAN1676, PBW343 and T. dicoccum DDK1001, respectively. Presence of the transgene hptII (hygromycin) in T
0 plants was confirmed by Southern hybridization. 相似文献