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941.
Hormonal regulation of fruit set, parthenogenesis induction and fruit expansion in Japanese pear 总被引:2,自引:1,他引:2
The effects of applied gibberellins (GAs), GA1, GA3, GA4 and GA7 with a cytokinin, N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) and indole-3-acetic acid (IAA) on fruit set, parthenogenesis induction and fruit expansion of a number
of Rosaceae species were assessed. These included Japanese pear cv. ‘Akibae’ (self-compatible) and cv. ‘Iwate yamanashi’ (a
seedless cultivar). Other Rosaceae species (Pyrus communis, Chaenomeles sinensis, Cydonia oblonga, and Malus pumila) were also investigated. GA4, GA7 and CPPU are very effective in inducing parthenocarpic fruit growth, whereas GA1, GA3 and IAA, have no ability to induce parthenogenesis in Japanese pear. GA4- and GA7-induced parthenocarpic fruit tended to be smaller in size, higher in flesh hardness, and showed advanced fruit ripening in
comparison to pollinated fruit and to parthenocarpic fruit induced by CPPU. GA4- and GA7-induced parthenocarpic fruit also had an increased pedicel length and fruit shape index and also showed a slight protrusion
of the calyx end. CPPU, GA4 and GA7 alone or combination with uniconazole were also active in inducing parthenogenesis in three other Rosaceae species, although
final fruit set was extremely low. GA1 was essentially inactive in promoting fruit expansion unlike the other bioactive GAs, whose effectiveness in promoting fruit
cell expansion was as follow: GA4 ≈ GA7 > GA3 > GA1. 相似文献
942.
943.
944.
摘要:【目的】本研究通过百日咳杆菌黏附素(PRN)基因的分段克隆表达及其在BALB/c小鼠的主动和被动免疫保护试验筛选PRN中的保护性抗原肽。【方法和结果】利用大肠杆菌进行PRN的完整蛋白、N端和C端多肽及其RI和RII区域多肽(双拷贝)的表达,命名为GST-PRN、GST-PN、GST-PC、GST-2PRI和GST-2PRII。Western blot检测证实5种表达产物均具有良好的反应原性。在主动免疫保护试验中,5种表达产物均能诱导小鼠产生较高的PRN抗体水平;当使用3 LD50的支气管败血波氏杆菌 相似文献
945.
Khaled M. Abdel Rahman Marek Barta Ľudovít Cagáň 《Central European Journal of Biology》2010,5(4):472-480
We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 × 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration
of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the
two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in
the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed
insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia. 相似文献
946.
The G-protein coupled receptor CCR5 functions pathologically as the primary co-receptor for macrophage tropic (R5) strains of HIV-1. The interactions responsible for co-receptor activity are unknown. Molecular-dynamics simulations of the extracellular and adjacent transmembrane domains of CCR5 were performed with explicit solvation utilizing a rhodopsin-based homology model. The functional unit of co-receptor binding was constructed via docking and molecular-dynamics simulation of CCR5 and the variable 3 loop of gp120, which is a dominant determinant of co-receptor utilization. The variable 3 loop was demonstrated to interact primarily with the amino terminus and the second extracellular loop of CCR5, providing novel structural information regarding the co-receptor-binding site. Alanine mutants that alter chemokine binding and co-receptor activity were examined. Molecular-dynamics simulations with and without the variable 3 loop of gp120 were able to rationalize the activities of these mutants successfully, providing support for the proposed model. Based on these results, the global complex of CCR5, gp120 including the V3 loop and CD4, was investigated. The utilization of computational analysis, in combination with molecular biological data, provides a powerful approach for understanding the use of CCR5 as a co-receptor by HIV-1. 相似文献
947.
Akinkurolere Rotimi Oluwafemi Qiong Rao Xi-Qiao Wang Hong-Yu Zhang 《Insect Science》2009,16(5):409-416
Abstract The suitability of combining microbial pesticides and an insect parasitoid for pest management of stored cereal in China was evaluated using laboratory assays. For this purpose, interactions between Bacillus thuringiensis ( Bt ), Bt -intoxicated host larvae and the parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) were tested against Plodia interpunctella Hübner (Lepidoptera: Pyralidae) . Bt or H. hebetor alone caused 41.67% and 35.35% P. interpunctella larval mortality respectively. The Bt –parasitoid combined treatment significantly increased mortality of P. interpunctella (86%). Progeny development of H. hebetor was dependent upon its susceptibility to Bt . Fewer parasitoids emerged from Bt –parasitoid combined treatment than in non- Bt treatments. However, since Bt did not prevent parasitoid development, a combined treatment with Bt and parasitoid release could produce better protection against P. interpunctella than either treatments when used singly, because their lethal effects were additive to each other. 相似文献
948.
Rodrigo J. Mercader Nathan W. Siegert Andrew M. Liebhold Deborah G. McCullough 《Population Ecology》2011,53(2):271-285
Management programs for invasive species are often developed at a regional or national level, but physical intervention generally
takes place over relatively small areas occupied by newly founded, isolated populations. The ability to predict how local
habitat variation affects the expansion of such newly founded populations is essential for efficiently targeting resources
to slow the spread of an invasive species. We assembled a coupled map lattice model that simulates the local spread of newly
founded colonies of the emerald ash borer (Agrilus planipennis Fairmaire), a devastating forest insect pest of ash (Fraxinus spp.) trees. Using this model, we investigated the spread of A. planipennis in environments with different Fraxinus spp. distributions, and explored the consequences of ovipositional foraging behavior on the local spread of A. planipennis. Simulations indicate that increased larval density, resulting from lower host tree density or higher initial population
sizes, can increase the spread rate during the first few years after colonization by increasing a density-dependent developmental
rate and via host resource depletion. Both the radial spread rate and population size were greatly influenced by ovipositional
foraging behavior. Two known behaviors of ovipositing A. planipennis females, attraction towards areas with high ash tree density and attraction to stressed trees, had opposing effects on spread.
Results from this model illustrate the significant influence of resource distribution and foraging behavior on localized spread,
and the importance of these factors when formulating strategies to monitor and manage invasive pests. 相似文献
949.
950.