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81.
Glycine decarboxylase is confined to the bundle-sheath cells of leaves of C3−C4 intermediate species
Christopher M. Hylton Stephen Rawsthorne Alison M. Smith D. Alan Jones Harold W. Woolhouse 《Planta》1988,175(4):452-459
Immunogold labelling has been used to determine the cellular distribution of glycine decarboxylase in leaves of C3, C3–C4 intermediate and C4 species in the genera Moricandia, Panicum, Flaveria and Mollugo. In the C3 species Moricandia foleyi and Panicum laxum, glycine decarboxylase was present in the mitochondria of both mesophyll and bundle-sheath cells. However, in all the C3–C4 intermediate (M. arvensis var. garamatum, M. nitens, M. sinaica, M. spinosa, M. suffruticosa, P. milioides, Flaveria floridana, F. linearis, Mollugo verticillata) and C4 (P. prionitis, F. trinervia) species studied glycine decarboxylase was present in the mitochondria of only the bundle-sheath cells. The bundle-sheath cells of all the C3–C4 intermediate species have on their centripetal faces numerous mitochondria which are larger in profile area than those in mesophyll cells and are in close association with chloroplasts and peroxisomes. Confinement of glycine decarboxylase to the bundle-sheath cells is likely to improve the potential for recapture of photorespired CO2 via the Calvin cycle and could account for the low rate of photorespiration in all C3–C4 intermediate species.Abbreviation and symbol kDa
kilodaltons
-
CO2 compensation point 相似文献
82.
Christopher A. Mitchell John K. McGeachie Miranda D. Grounds 《Cell and tissue research》1992,269(1):159-166
Summary Skeletal muscle regeneration in SJL/J and BALB/c mice subjected to identical crush injuries is markedly different: in SJL/J mice myotubes almost completely replace damaged myofibres, whereas BALB/c mice develop fibrotic scar tissue and few myotubes. To determine the cellular changes which contribute to these differential responses to injury, samples of crushed tibialis anterior muscles taken from SJL/J and BALB/c mice between 1 and 10 days after injury were analysed by light and electron microscopy, and by autoradiography. Longitudinal muscle sections revealed about a 2-fold greater total mononuclear cell density in SJL/J than BALB/c mice at 2 to 3 days after injury. Electron micrographs identified a similar proportion of cell types at 3 days after injury. Autoradiographic studies showed that the proportions of replicating mononuclear cells in both strains were similar: therefore greater absolute numbers of cells (including muscle precursors and macrophages) were proliferating in SJL/J muscle. Removal of necrotic muscle debris in SJL/J mice was rapid and extensive, and by 6 to 8 days multinucleated myotubes occupied a large part of the lesion. By contrast, phagocytosis was less effective in BALB/c mice, myotube formation was minimal, and fibrotic tissue conspicuous. These data indicate that the increased mononuclear cell density, more efficient removal of necrotic muscle, together with a greater capacity for myotube formation in SJL/J mice, contribute to the more successful muscle regeneration seen after injury. 相似文献
83.
Christopher H. Hann 《Dialectical Anthropology》1992,17(2):141-166
Conclusion In concluding, let us restate the main theoretical arguments concerning Austria-Hungary and Malinowski-Polanyi. There are many more aspects of this latter contrast which I have not dwelt on, including those of temper and personality. My concern was to show that it is not enough to trace ideas back to their origin in an undifferentiated Central Europe, for we need to be quite clear about the context in which those ideas were initially taken on board. Mach was an appropriate philosopher for the ordered bureaucracy of Austria, and he embodies the individual-centered and fundamentally conservative message, which I see articulated later in very different (and non-positivist) styles by Popper, Hayek, Schumpeter, etc. This was the message accepted in its fundamentals by Malinowski in Cracow. The Hungarian half of the Empire had quite different traditions, and one might even say that political dilemmas in this half of the Empire were so pressing that they had no time for philosophy. By the time Polanyi was growing up in Budapest, nationalist fervor was at its height, and a nineteenth century heritage of liberal, even radical thought, was under pressure from the extreme right. Polanyi's Jewish family background probably pushed him towards the Left in his early years, though it in no way interfered with this Hungarian patriotism. When he read Mach, he accepted the individualist and positivist aspects of the message, but he could never endorse the conservative respect for tradition that underpinned Malinowski's functionalism. This is what makes his position so interesting, and he maintained it throughout his career.In this light, Polanyi's economic anthropology is an authentic cultural product of Central Europe. Malinowski, to the extent that he took on board the basic social theory of Austrian liberal conservatism, sold out to the West, and to an ideological tradition which has an alternative home in another country on the periphery of industrial capitalism: Enlightenment Scotland. Austria-Hungary also produced intellectuals who sold out in the other direction, and abandoned all notion of intellectual autonomy for the individual by capitulating to the total discipline of the revolutionary Party. This tradition is that of Russia and Lenin, and the best known Central European intellectual recruit was Lukács, perhaps because he studied idealists in Germany rather than the positivists in Vienna. Karl Polanyi was unable to give unswerving loyalty either to the capitalist ideology of the West, or to the ideology of the new tributary mode of production in the East. The middle position, which I believe he occupied throughout his life, is one that will never satisfy ideologues, and it is hard to infuse it with the same intellectual elegance. Still, just as his historical researches showed the real possibilities for combining modes of economic integration, and his life experiences showed the folly of concentration on only one, so Hungarians have learned from history that they are best off with the sort of mixed economy they have today. His popularity in Hungary is therefore unsurprising, and one may hope that the practical message of his anthropology will not be permanently forgotten in the West.Christopher H. Hann is a Professor of Social Anthropology, University of Kent, Canterbury. 相似文献
84.
John Greenman Nancy Hogg Suzanne Nikoletti Christopher Slade George Stevenson Martin Glennie 《Cancer immunology, immunotherapy : CII》1992,34(6):361-369
Summary The three forms of Fc receptor carried by monocytes (FcRI, II) and natural killer (NK) cells (FcRIII) are all capable of mediating cell lysis. Here we compare the use of F(ab)2 bispecific antibodies, specifically targetting individual FcR, and chimeric IgG mouse/human antibodies which are capable of targetting all FcR, for their ability to mediate target cell destruction. The derivatives are prepared by linking hinge sulphydryl residues via tandem thioether bonds, using a bismaleimide crosslinker: Fab from an anti-FcR mAb linked to Fab from a common anti-target mAb (BsAb), or Fab from the common anti-target mouse antibody linked to human Fc (FabFc or bisFabFc). All the derivatives targetting chick red blood cells gave efficient lysis, although different effector cell donors yielded differences in both the lytic levels achieved and the comparative efficiencies of derivatives. In contrast, significant lysis of the guinea pig lymphoblastic leukaemia, L2C, regularly resulted only via the anti-FcRIII BsAb and the chimeric derivatives. These results suggest that the chimeric, Fc-containing derivatives mediate tumour cell lysis principally through FcRIII on NK cells. This is in contrast to the situation with the chick red blood cells where the chimeric derivatives appear capable of lysing erythrocytes by utilizing either monocytes or NK cells, because significant (50%) lysis occurred with effector cell populations magnetically depleted through either FcRII or FcRIII. A major difference between these two types of antibody derivative was their ability to function in the presence of high concentrations of normal human Fc. The lysis mediated by BsAb reactive with FcRI or II was unaffected by the presence of human Fc at 2.5 mg/ml (a concentration comparable with that yielded by IgG in plasma) whereas the BsAb recognizing FcRIII and all the Fc-containing derivatives were completely inhibited.This work has been supported by Tenovus, the Cancer Research Campaign, the Leukaemia Research Fund, Italfarmaco, Milano, Italy and the Imperial Cancer Research Fund 相似文献
85.
Shannon R. Barber John Werdel Millie Symbula John Williams Barbara A. Burkett Peyton T. Taylor James K. Roche Christopher P. Crum 《Cancer immunology, immunotherapy : CII》1992,35(1):33-38
Summary Although serological reactivity to human papillomavirus type 16 (HPV-16) proteins has been demonstrated in patients with invasive cervical carcinoma, the degree of seroreactivity to these proteins in women with preinvasive disease and its relationship to the HPV type associated with the disease are unclear. We obtained sera from 27 women undergoing cone biopsy for cervical precursor lesions and 22 controls and analyzed seroreactivity by Western blot to fusion proteins containing portions of the HPV-16 E4, L1 and L2 open-reading frames (ORFs). Positives were analyzed by scanning densitometry and intensity values for each case plotted relative to controls. Cervical biopsy specimens from patients were analyzed for HPV-16 nucleic acids by DNA · DNA in situ hybridization. Mean intensity values for seroreactivity to the pATH-E4 protein approached significance (P = 0.058) and a significantly higher proportion of cases vs controls registered values over 4.0 for pATH-E4 (26% vs 4.5%;P = 0.04) and pATH-L2 (48% vs 18%;P = 0.03) proteins. A significantly higher mean intensity value for E4 was observed for cases containing HPV-16 DNA vs HPV-16 negative cases or controls. Thus, seroreactivity to HPV-16-derived proteins may be more common in women with preinvasive cervical disease, and for some protein targets (E4) may indicate a relatively type-specific response.Supported in part by grants from the National Cancer Institute [CA 47676 (C.P.C.)], American Cancer Society [MV-395 (C.P.C.)] and an institutional support grant (J.K.R.). Dr. Crum is a recipient of a Physician Scientist Award from the National Institute of Allergy and Infectious Disease (AI00628) 相似文献
86.
Christopher S. Colwell Sat Bir S. Khalsa Gene D. Block 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1992,170(2):211-215
Summary The eye of the marine mollusk Aplysia californica contains a photo-entrainable circadian pacemaker that drives an overt circadian rhythm of spontaneous compound action potentials in the optic nerve. Both light and serotonin are known to influence the phase of this ocular rhythm. The current study evaluated the effect of FMRFamide on both light and serotonin induced phase shifts of this rhythm. The application of FMRFamide was found to block serotonin induced phase shifts but, by itself, FMRFamide did not cause significant phase shifts. Furthermore, the effects of FMRFamide on light-induced phase shifts appeared to be phase dependent (i.e., the application of FMRFamide inhibited light-induced phase delays but actually enhanced the magnitude of phase advances). As in Aplysia, the eye of Bulla gouldiana also contains a circadian pacemaker. In Bulla, FMRFamide prevented light-induced phase advances and delays. Although FMRFamide alone generated phase dependent phase shifts, it did not cause phase shifts at the phases where it blocked the effects of light. These data demonstrate that FMRFamide can have pronounced modulatory effects on phase shifting inputs to the ocular pacemakers of both Aplysia and Bulla.Abbreviations
ASW
artificial seawater
-
CAP
compound action potential
-
CT
circadian time
-
5-HT
serotonin 相似文献
87.
In the Lewis rat immunisation with the myelin PO glycoprotein can induce an inflammatory demyelinating disease of the peripheral nervous system, experimental allergic neuritis (EAN), which has many clinical and histopathological parallels with the human disease the Guillain-Barre syndrome. In view of the reported association of GBS with a number of infectious agents we have investigated whether molecular mimicry may occur between microbial antigens and the PO protein that could possibly trigger a similar pathogenic autoimmune response in man. A computer search of the available protein sequence data bases identified several absolute sequence homologies between PO and viral proteins that involve five or more consecutive amino acid residues. Four of these sequence homologies involved viral pathogens previously associated with the Guillain-Barre syndrome, namely Epstein-Barr virus (EBV), cytomegalovirus (CMV), Varicella zoster virus (VZV) and human immunodeficiency virus I (HIV I). Although, sequence homologies were also found between viral peptides and the neuritogenic determinants of PO, residues 56–71 and 180–199, these homologies proved incapable of eliciting EAN in the Lewis rat. These observations are discussed with reference to the role that molecular mimicry between T cell epitopes on pathogen derived antigens and the PO protein may play in the pathogenesis of the Guillain-Barre syndrome.Abbreviations EAN
Experimental allergic neuritis
- EAE
experimental allergic encephalomyelitis
- PNS
peripheral nervous system
- CNS
central nervous system
- MBP
myelin basic protein
- GBS
Guillain Barre syndrome
- CFA
complete Freund's Adjuvant
- LPC
lysophosphatidyl choline
- VZV
Varicella zoster virus
- CMV
cytomegalovirus
- EBV
Epstein Barr virus
- HIV I
human immunodeficiency virus I
Special issue dedicated to Dr. Alan N. Davison 相似文献
88.
89.
90.
Gerard H. Markx Christopher L. Davey Douglas B. Kell Phillip Morris 《Journal of biotechnology》1991,20(3):279-290
A novel technique is described for the measurement of the volume fraction of biomass in a suspension by the simultaneous measurement of the conductivity of a suspension containing cells and of the medium in which the cells are suspended. The presence of non-conducting particulate matter in a suspension will cause the conductivity of a suspension to be decreased relative to that of the medium in which the particles are suspended. A simple equation (the Bruggeman equation) describes the relationship between the volume fraction of non-conducting particulate matter and the decrease in conductivity. The accuracy of this method for the determination of the biomass concentration of plant cells (Festuca arundinacea) in culture was shown. The method was successfully applied to the on-line determination of biomass concentrations during the growth of F. arundinacea cultures, and gave good agreement with biomass levels as determined from measurements of the radio-frequency dielectric permittivity of such cultures. 相似文献