全文获取类型
收费全文 | 6411篇 |
免费 | 598篇 |
国内免费 | 2篇 |
专业分类
7011篇 |
出版年
2024年 | 7篇 |
2023年 | 44篇 |
2022年 | 74篇 |
2021年 | 123篇 |
2020年 | 90篇 |
2019年 | 111篇 |
2018年 | 163篇 |
2017年 | 126篇 |
2016年 | 225篇 |
2015年 | 391篇 |
2014年 | 414篇 |
2013年 | 481篇 |
2012年 | 612篇 |
2011年 | 558篇 |
2010年 | 358篇 |
2009年 | 298篇 |
2008年 | 389篇 |
2007年 | 384篇 |
2006年 | 340篇 |
2005年 | 305篇 |
2004年 | 340篇 |
2003年 | 288篇 |
2002年 | 254篇 |
2001年 | 41篇 |
2000年 | 38篇 |
1999年 | 58篇 |
1998年 | 83篇 |
1997年 | 41篇 |
1996年 | 29篇 |
1995年 | 45篇 |
1994年 | 32篇 |
1993年 | 31篇 |
1992年 | 32篇 |
1991年 | 16篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 20篇 |
1987年 | 8篇 |
1986年 | 7篇 |
1985年 | 10篇 |
1984年 | 8篇 |
1983年 | 13篇 |
1982年 | 12篇 |
1981年 | 10篇 |
1980年 | 11篇 |
1979年 | 4篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 9篇 |
1974年 | 9篇 |
排序方式: 共有7011条查询结果,搜索用时 0 毫秒
991.
992.
993.
Mycophenolic acid reverses TGF beta‐induced cell motility,collagen matrix contraction and cell morphology in vitro 下载免费PDF全文
Christian Koch Frank Christian Schultze Christoph Eberle Philip D. Walson Michael Oellerich 《Cell biochemistry and function》2015,33(7):503-508
The aim of this study was to elucidate functional and molecular effects of mycophenolic acid (MPA) on non‐lymphatic, kidney epithelial cells treated with transforming growth factor (TGF). MPA effects were studied using HK2 cells incubated with EGF and TGF. The reversibility of these effects was verified using guanosine and 8‐aminoguanosine. The following assays were applied: cell proliferation, viability, collagen matrix contraction, scratch wound closure, spindle index, FACS with anti‐CD29 and anti‐CD326, promoter demethylation of RAS protein activator like 1 (RASAL1), as well as gene expression of RASAL1, integrin 1ß (ITGB1) (CD29) and epithelial cell adhesion molecule (EpCam) (CD326). Cell proliferation was inhibited by increasing concentrations of MPA, whereas neither apoptosis nor cytotoxicity was detected. Stimulation with EGF and/or TGF led to a significant collagen matrix contraction that was successfully inhibited by MPA. In addition, scratch wound closure was inhibited by incubation with TGF alone or with EGF. Under the same conditions, cell morphology (spindle shape) and molecular phenotype (ITGB1HighEpCamLow/ITGB1LowEpCamHigh) were both significantly changed, suggesting an epithelial to mesenchymal transformation. Cell morphology and motility, as well as molecular phenotype, were reversible after MPA treatment with TGF transformation in both presence/absence of EGF, thereby suggesting a correlation with the previously described antifibrotic effects of MPA. Dysregulation of TGF signal transduction appears to be related to progression of fibrosis. A TGF‐transformed kidney epithelial cell line derived from human proximal tubules was used to study whether the immunosuppressive drug: MPA possesses any functional or molecular antifibrotic effects. Functional and morphological in vitro changes induced by both the TGF and epithelial‐growth‐factor were reversible by treatment with MPA. An inhibitory effect of MPA on the TGF pathway appears to be responsible for the previously described antifibrotic effects of the MPA in the COL4A3‐deficient mouse model of renal fibrosis. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
994.
Gabriel St?lting Stefanie Bungert-Plümke Arne Franzen Christoph Fahlke 《The Journal of biological chemistry》2015,290(51):30406-30416
ClC-K chloride channels are crucial for auditory transduction and urine concentration. Mutations in CLCNKB, the gene encoding the renal chloride channel hClC-Kb, cause Bartter syndrome type III, a human genetic condition characterized by polyuria, hypokalemia, and alkalosis. In recent years, several Bartter syndrome-associated mutations have been described that result in truncations of the intracellular carboxyl terminus of hClC-Kb. We here used a combination of whole-cell patch clamp, confocal imaging, co-immunoprecipitation, and surface biotinylation to study the functional consequences of a frequent CLCNKB mutation that creates a premature stop codon at Trp-610. We found that W610X leaves the association of hClC-Kb and the accessory subunit barttin unaffected, but impairs its regulation by barttin. W610X attenuates hClC-Kb surface membrane insertion. Moreover, W610X results in hClC-Kb channel opening in the absence of barttin and prevents further barttin-mediated activation. To describe how the carboxyl terminus modifies the regulation by barttin we used V166E rClC-K1. V166E rClC-K1 is active without barttin and exhibits prominent, barttin-regulated voltage-dependent gating. Electrophysiological characterization of truncated V166E rClC-K1 demonstrated that the distal carboxyl terminus is necessary for slow cooperative gating. Since barttin modifies this particular gating process, channels lacking the distal carboxyl-terminal domain are no longer regulated by the accessory subunit. Our results demonstrate that the carboxyl terminus of hClC-Kb is not part of the binding site for barttin, but functionally modifies the interplay with barttin. The loss-of-activation of truncated hClC-Kb channels in heterologous expression systems fully explains the reduced basolateral chloride conductance in affected kidneys and the clinical symptoms of Bartter syndrome patients. 相似文献
995.
996.
997.
Neele Schumacher D?rte Meyer Andre Mauermann Jan von der Heyde Janina Wolf Jeanette Schwarz Katharina Knittler Gillian Murphy Matthias Michalek Christoph Garbers J?rg W. Bartsch Songbo Guo Beate Schacher Peter Eickholz Athena Chalaris Stefan Rose-John Bj?rn Rabe 《The Journal of biological chemistry》2015,290(43):26059-26071
Generation of the soluble interleukin-6 receptor (sIL-6R) is a prerequisite for pathogenic IL-6 trans-signaling, which constitutes a distinct signaling pathway of the pleiotropic cytokine interleukin-6 (IL-6). Although in vitro experiments using ectopically overexpressed IL-6R and candidate proteases revealed major roles for the metalloproteinases ADAM10 and ADAM17 in IL-6R shedding, the identity of the protease(s) cleaving IL-6R in more physiological settings, or even in vivo, remains unknown. By taking advantage of specific pharmacological inhibitors and primary cells from ADAM-deficient mice we established that endogenous IL-6R of both human and murine origin is shed by ADAM17 in an induced manner, whereas constitutive release of endogenous IL-6R is largely mediated by ADAM10. Although circulating IL-6R levels are altered in various diseases, the origin of blood-borne IL-6R is still poorly understood. It has been shown previously that ADAM17 hypomorphic mice exhibit unaltered levels of serum sIL-6R. Here, by quantification of serum sIL-6R in protease-deficient mice as well as human patients we also excluded ADAM10, ADAM8, neutrophil elastase, cathepsin G, and proteinase 3 from contributing to circulating sIL-6R. Furthermore, we ruled out alternative splicing of the IL-6R mRNA as a potential source of circulating sIL-6R in the mouse. Instead, we found full-length IL-6R on circulating microvesicles, establishing microvesicle release as a novel mechanism for sIL-6R generation. 相似文献
998.
999.
Kole T. Roybal Emily M. Mace Danielle J. Clark Alan D. Leard Andrew Herman Paul Verkade Jordan S. Orange Christoph Wülfing 《PloS one》2015,10(8)
Dynamic subcellular distributions of signaling system components are critical regulators of cellular signal transduction through their control of molecular interactions. Understanding how signaling activity depends on such distributions and the cellular structures driving them is required for comprehensive insight into signal transduction. In the activation of primary murine T cells by antigen presenting cells (APC) signaling intermediates associate with various subcellular structures, prominently a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell. While actin dynamics are well established as general regulators of cellular organization, their role in controlling signaling organization in primary T cell:APC couples and the specific cellular structures driving it is unresolved. Using modest interference with actin dynamics with a low concentration of Jasplakinolide as corroborated by costimulation blockade we show that T cell actin preferentially controls lamellal signaling localization and activity leading downstream to calcium signaling. Lamellal localization repeatedly related to efficient T cell function. This suggests that the transient lamellal actin matrix regulates T cell signaling associations that facilitate T cell activation. 相似文献
1000.
Christopher Boehlke Heike Janusch Christoph Hamann Christian Powelske Miriam Mergen Henriette Herbst Fruzsina Kotsis Roland Nitschke E. Wolfgang Kuehn 《PloS one》2015,10(10)
Ift88 is a central component of the intraflagellar transport (Ift) complex B, essential for the building of cilia and flagella from single cell organisms to mammals. Loss of Ift88 results in the absence of cilia and causes left-right asymmetry defects, disordered Hedgehog signaling, and polycystic kidney disease, all of which are explained by aberrant ciliary function. In addition, a number of extraciliary functions of Ift88 have been described that affect the cell-cycle, mitosis, and targeting of the T-cell receptor to the immunological synapse. Similarly, another essential ciliary molecule, the kinesin-2 subunit Kif3a, which transports Ift-B in the cilium, affects microtubule (MT) dynamics at the leading edge of migrating cells independently of cilia. We now show that loss of Ift88 impairs cell migration irrespective of cilia. Ift88 is required for the polarization of migrating MDCK cells, and Ift88 depleted cells have fewer MTs at the leading edge. Neither MT dynamics nor MT nucleation are dependent on Ift88. Our findings dissociate the function of Ift88 from Kif3a outside the cilium and suggest a novel extraciliary function for Ift88. Future studies need to address what unifying mechanism underlies the different extraciliary functions of Ift88. 相似文献