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61.
62.
Attempts to induce enantioselectivity in the catalytic hydrogenation of unsymmetrically substituted aromatics using covalently bound, well known chiral auxiliaries are described. Marked differences in stereoselectivity and rate of hydrogenolysis are noted as a function of the auxiliary used. Enantioselectivities obtained in the resulting cyclohexyl derivatives are rather poor. © 1995 Wiley-Liss, Inc. 相似文献
63.
Three endocyclic sulfoximides of the 1-aryl- and 1-alkyl-3-oxo-benzo[d]-isothia (IV)-azole 1-oxide type (1-substituent = 2′-carboxyphenyl, 2′-carbethoxyphenyl, and octyl, respectively) were found to be well resolved on a chiral phase derived from bovine serum albumin (BSA). Selectivities (α) of 1.74, 1.12, and 1.44, respectively, were obtained. The retention behaviour of 1-octyl-3-oxo-benzo[d]isothia(IV)-azole 1-oxide was further investigated in some detail as a function of the mobile phase composition and the elution order was established from optically active material obtained from the enantiopure sulfoxide precursor. An enantiomeric excess of 85.4% was obtained in the cyclocondensation reaction of the octyl-substituted sulfoxide precursor with hydrazoic acid to the corresponding endocyclic sulfoximide. © 1995 Wiley-Liss, Inc. 相似文献
64.
Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination 总被引:38,自引:0,他引:38
An integron is a genetic unit that includes the determinants of the components of a site-specific recombination system capable of capturing and mobilizing genes that are contained in mobile elements called gene cassettes. An integron also provides a promoter for expression of the cassette genes, and integrons thus act both as natural cloning systems and as expression vectors. The essential components of an integron are an int gene encoding a site-specific recombinase belonging to the integrase family, an adjacent site, attl, that is recognized by the integrase and is the receptor site for the cassettes, and a promoter suitably oriented for expression of the cassette-encoded genes. The cassettes are mobile elements that include a gene (most commonly an antibiotic-resistance gene) and an integrase-specific recombination site that is a member of a family of sites known as 59-base elements. Cassettes can exist either free in a circularized form or integrated at the attl site, and only when integrated is a cassette formally part of an integron. A single site-specific recombination event involving the integron-associated attl site and a cassette-associated 59-base element leads to insertion of a free circular cassette into a recipient integron. Multiple cassette insertions can occur, and integrons containing several cassettes have been found in the wild. The integrase also catalyses excisive recombination events that can lead to loss of cassettes from an integron and generate free circular cassettes. Due to their ability to acquire new genes, integrons have a clear role in the evolution of the genomes of the plasmids and transposons that contain them. However, a more general role in evolution is also likely. Events involving recombination between a specific 59-base-element site and a nonspecific secondary site have recently been shown to occur. Such events should lead either to the insertion of cassettes at non-specific sites or to the formation of stable cointegrates between different plasmid molecules, and a cassette situated outside the integron context has recently been identified. 相似文献
65.
66.
Two methods (whole-plant growth analysis and gas exchange) were used to measure the response of Psophocarpus tetragonolobus (L.) DC cultivar UPS 99 to the environment. This plant had an optimal temperature for root growth of 25°C, its rate of acetylene reduction (when inoculated with Rhizobium, strain RRIM 56) was maximal at 30°C and it required an atmospheric temperature of about 35°C for optimal shoot growth. Maximum water-use efficiency was ca. 33 mg CO2·g H2O-1. The rate of photosynthesis reached a plateau at 900 vpm CO2-this condition also gave the lowest rate of transpiration. Under normal conditions, the light compensation point was at 1.7 klx, while that for CO2 was 60 vpm. Photorespiration diminished gross photosynthesis of P. tetragonolobus by forty percent. Water stress (as measured by sensitivity to slightly increased CO2 levels) caused rapid closure of stomata, and the response was remembered for up to five days.
Zusammenfassung Mit Hilfe von zwei Methoden (Wachstumsanalysen ganzer Pflanzen und Gaswechselmessungen) wurde die Reaktion von Psophocarpus tetragonolobus (L.) DC der Sorte UPS 99 auf Umwelteinflüsse ermittelt. 25°C war die optimale Temperatur für das Wurzelwachstum. Die Acetylenreduktionsrate (die Pflanzen waren geimpft worden mit Rhizobium RRIM 56) war am höchsten bei 30°C. 35°C waren notwendig für maximales Sproßwachstum. Der günstigste Wasserausnutzungskoeffizient lag bei ungefähr 33 (mg CO2·g H2O-1). Die Photosyntheseraten wurden durch Erhöhung der CO2-Konzentration gesteigert. Bei Konzentrationen über 900 vpm CO2 konnte allerdings keine weitere Steigerung mehr festgestellt werden. Bei 900 vpm CO2 waren die Transpirationsraten am niedrigsten. Unter normalen Bedingungen stellte sich der Lichtkompensationspunkt bei 1,7 klx ein. Der CO2-Kompensationspunkt lag bei 60 vpm CO2. Die Photorespiration verminderte die Photosynthese von P. tetragonolobus um 40%. Wasserstreß vergrößerte die Empfindlichkeit der Stomata gegenüber etwas erhöhten CO2-Konzentrationen (die Stomata schließen). Diese Empfindlichkeit war bis zu 5 Tagen nach der Streßbehandlung noch meßbar.相似文献
67.
Albert de la Chapelle Christina Kari Markku Nurminen Sven Hernberg 《Human genetics》1977,37(2):183-194
Summary An epidemic of agranulocytosis and granulocytopenia occurred in 1975 in conjunction with clozapine treatment of mental patients in Finland. An attempt was made to assess the epidemiologic and genetic factors contributing to the adverse drug effect. The estimated incidence rate in Finland was 2.1/1000 patient-months. This figure could not be compared with rates from other countries because of the inexact nature of the figures reported so far. All 16 cases occurred in seven hospitals in southwestern Finland, whereas the overall hospital net use of the drug was geographically evenly distributed. The difference between the observed and the proportionally expected incidence of cases amongst the hospitals where clozapine was used was statistically significant. The average consumption of the drug did not differ between the hospitals where cases occurred and those where no definite cases could be diagnosed. Six-generation pedigree analyses failed to reveal significant parental consanguinity or genetic kinship between probands. Neither did the birth places of the ancestors of the probands disclose a typical isolate pattern. In conclusion, the cases appeared to be confined to a few hospitals in southwestern Finland. Although a genetic factor is not excluded, we found no evidence in support of a genetic mechanism. 相似文献
68.
Christina M. Hansen Bay 《Journal of insect physiology》1978,24(2):141-149
The salivary glands of adult Calliphora contain enzymes which hydrolyze starch, sucrose and trehalose. Amylase and sucrase are shown to be secretory enzymes, while trehalase remains in the gland. Results of electrophoretic and ultrastructural studies suggest that protein secretion is confined to the abdominal region of the gland. Secretion of both fluid and protein occurs from a single type of cell. While a fly is feeding on solid sugar, amylase and sucrase are lost from the gland and appear in saliva, while the level of trehalase in the gland increases slightly. The mixture of food and saliva passes mainly to the crop where carbohydrate is digested by the salivary enzymes. 相似文献
69.
70.
T Lenz E Werle G Strobel H Weicker 《Canadian journal of physiology and pharmacology》1991,69(7):929-937
The physiological effects of the sulfoconjugates of epinephrine, norepinephrine, and the 3-O-methylated catecholamines, metanephrine, normetanephrine, and methoxytyramine were examined with regard to their alpha 2-adrenoceptor binding properties and aggregation activity in human platelets. Sulfoconjugation of catecholamines resulted in the loss of both their competitive potency for [3H]yohimbine binding and their influence on platelet aggregation. O-Methyl substituted catecholamines showed attenuation of their alpha 2-adrenoceptor binding affinities when compared with those of the corresponding non-esterified amines. Unlike the free amine epinephrine, which stimulated platelet aggregation, the O-methylated catecholamine derivatives inhibited aggregation. Inhibition was dose-dependent and restricted to the alpha 2-adrenoceptor mediated aggregation response stimulated by epinephrine (1 microM) or potentiated by subthreshold concentrations of epinephrine (30-300 nM) in the presence of subaggregatory doses of vasopressin (10-30 nM). Collagen- and ADP-induced platelet aggregation was not affected. The hydrophilic beta-antagonist CGP 12177 displayed no effects. However, high concentrations (0.1 mM) of both isomers of the strongly lipophilic beta-adrenoceptor antagonist propranolol inhibited the actions of all aggregators by stabilizing the membrane. Such a nonspecific membrane interaction of the methylated catecholamines could be excluded because of their low lipid solubility calculated in a n-octanol-phosphate buffer system at pH 7.4. We suggest therefore that methylated catecholamines are biological alpha 2-adrenoceptor antagonists acting on alpha 2-adrenoceptor stimulated reactions of human platelets. Whether this receptor antagonism is relevant to other human tissues needs clarification. Sulfated catecholamines, however, are wholly ineffective at this receptor site and may constitute a pathway to control the concentration of the active free catecholamines. 相似文献