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991.
Christiane Stark Stefan G. Michalski Wiesław Babik Grit Winterfeld Walter Durka 《Plant Systematics and Evolution》2011,293(1-4):213-226
The fragrant orchid Gymnadenia conopsea s.l. is a controversial taxon with two commonly distinguished species, G. conopsea s.str. and G. densiflora. Despite morphological similarity, differentiation between the taxa has been reported for several characters; however, character variation within taxa has obviated a clear consensus. We assessed ITS sequences, microsatellite variation and chromosome numbers on the European scale (1,420 samples) and conducted morphological analyses for 626 samples from Germany. ITS analysis revealed a 2% nucleotide divergence between the taxa, similar to the divergence between other Gymnadenia species. The ITS sequences of G. densiflora form a well-supported monophyletic group sharing a most recent common ancestor with G. nigra and G. austriaca. Thus, G. conopsea and G. densiflora are not sister species, and a species rank is supported for G. densiflora (Wahlenb.) Dietrich and G. conopsea (L.) R.Br. s.str. This was confirmed by the microsatellite analysis, which revealed a strong genetic differentiation between the taxa because of largely non-overlapping sets of alleles. Chromosome numbers showed that G. conopsea was either diploid or tetraploid, whereas G. densiflora was diploid throughout. Morphologically, the taxa differed significantly in the mean value of a number of diagnostic characters. However, a discriminant analysis showed that the morphological variability is substantial, and on the individual level an unequivocal assignment is not possible as 96% of G. conopsea, but only 77% of G. densiflora could be assigned correctly. Further studies are needed on character variation within and among species and ploidy levels to allow for a better identification of the genetically differentiated but morphologically similar taxa. 相似文献
992.
Fusarium head blight (FHB) caused by Fusarium graminearum and F. culmorum is a devastating disease with high effects on grain yield and quality. We developed spring wheat lines incorporating the highly effective FHB resistance quantitative trait loci (QTL) Fhb1 and Qfhs.ifa‐5A. Whether these QTL lead to competition within Fusarium populations in the field resulting in isolates with higher aggressiveness has not been analysed. The aims of this study were to determine (i) the aggressiveness potential of F. graminearum and F. culmorum isolates, (ii) competition effects of these isolates in binary mixtures and (iii) the stability of resistant hosts. Six F. graminearum, two F. culmorum isolates and seven binary mixtures containing these isolates were tested for their aggressiveness and mycotoxin production at two locations in South Germany in 2007 and 2008. Host lines were four spring wheat lines containing the resistance QTL Fhb1 and/or Qfhs.ifa‐5A or none of them and one standard variety. Re‐isolates were sampled from plots inoculated with the binary mixtures to identify the percentage of each isolate in the mixture by simple sequence repeat markers. Resistant host lines reacted as expected and had a high stability to all isolates and mixtures. Only less important host × mixture interactions were detected. Aggressiveness among isolates and mixtures was significantly different. Type and amount of mycotoxin and high single isolate aggressiveness were not necessarily advantageous in the mixture. However, both F. culmorum isolates outcompeted F. graminearum isolates. Significant deviations from the inoculated 1 : 1 proportions occurred in 34 of 49 cases, illustrating that competition effects appeared in the mixtures. These differences depended mainly on the year and not on the level of host resistance. We conclude that resistance should not be affected by the Fusarium isolates and mixtures. 相似文献
993.
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system which responds to anti-inflammatory treatments in the early disease phase. However, the pathogenesis of the progressive disease phase is less well understood, and inflammatory as well as neurodegenerative mechanisms of tissue damage are currently being discussed. This review summarizes current knowledge on the interrelation between inflammation, demyelination, and neurodegeneration derived from the study of human autopsy and biopsy brain tissue and experimental models of MS. 相似文献
994.
Effects of ensiling, silage additives and storage period on methane formation of biogas crops 总被引:7,自引:0,他引:7
Effects of the ensiling process, storage periods of up to 1 year and several chemical and biological silage additives on biomethanation were assessed for maize, sorghum, forage rye and triticale with the aim to identify optimised conditions for silage production of crops used as feedstock in biogas plants. Ensiling, prolonged storage and biological silage additives showed positive effects on methane yield of up to 11%. These could be attributed to increases in organic acids and alcohols during ensiling. A regression model including acetic acid, butyric acid and ethanol accounts for 75-96% of the variation in methane yield. Storage periods of up to 1 year for properly ensiled crops could be possible without losses in methane production, considering the increase in methane yield and the losses of dry matter during this period. However, taking storage losses into account silage additives showed little effect on methane production. 相似文献
995.
The PsbW protein stabilizes the supramolecular organization of photosystem II in higher plants 总被引:1,自引:0,他引:1
García-Cerdán JG Kovács L Tóth T Kereïche S Aseeva E Boekema EJ Mamedov F Funk C Schröder WP 《The Plant journal : for cell and molecular biology》2011,65(3):368-381
PsbW, a 6.1-kDa low-molecular-weight protein, is exclusive to photosynthetic eukaryotes, and associates with the photosystem II (PSII) protein complex. In vivo and in vitro comparison of Arabidopsis thaliana wild-type plants with T-DNA insertion knock-out mutants completely lacking the PsbW protein, or with antisense inhibition plants exhibiting decreased levels of PsbW, demonstrated that the loss of PsbW destabilizes the supramolecular organization of PSII. No PSII-LHCII supercomplexes could be detected or isolated in the absence of the PsbW protein. These changes in macro-organization were accompanied by a minor decrease in the chlorophyll fluorescence parameter F(V) /F(M) , a strongly decreased PSII core protein phosphorylation and a modification of the redox state of the plastoquinone (PQ) pool in dark-adapted leaves. In addition, the absence of PsbW protein led to faster redox changes in the PQ pool, i.e. transitions from state 1 to state 2, as measured by changes in stationary fluorescence (F(S) ) kinetics, compared with the wild type. Despite these dramatic effects on macromolecular structure, the transgenic plants exhibited no significant phenotype under normal growth conditions. We suggest that the PsbW protein is located close to the minor antenna of the PSII complex, and is important for the contact and stability between several PSII-LHCII supercomplexes. 相似文献
996.
Fader LD Bethell R Bonneau P Bös M Bousquet Y Cordingley MG Coulombe R Deroy P Faucher AM Gagnon A Goudreau N Grand-Maître C Guse I Hucke O Kawai SH Lacoste JE Landry S Lemke CT Malenfant E Mason S Morin S O'Meara J Simoneau B Titolo S Yoakim C 《Bioorganic & medicinal chemistry letters》2011,21(1):398-404
997.
Xia G Corrigan RM Winstel V Goerke C Gründling A Peschel A 《Journal of bacteriology》2011,193(15):4006-4009
The molecular interactions between staphylococcal phages and host cell surfaces are poorly understood. Employing Staphylococcus aureus teichoic acid mutants, we demonstrate that wall teichoic acid (WTA), but not lipoteichoic acid, serves as a receptor for staphylococcal siphovirus and myovirus, while only the siphovirus requires glycosylated WTA. 相似文献
998.
Liebminger E Veit C Pabst M Batoux M Zipfel C Altmann F Mach L Strasser R 《The Journal of biological chemistry》2011,286(12):10793-10802
Most plant glycoproteins contain substantial amounts of paucimannosidic N-glycans instead of their direct biosynthetic precursors, complex N-glycans with terminal N-acetylglucosamine residues. We now demonstrate that two β-N-acetylhexosaminidases (HEXO1 and HEXO3) residing in different subcellular compartments jointly account for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. Total N-glycan analysis of hexo knock-out plants revealed that HEXO1 and HEXO3 contribute equally to the production of paucimannosidic N-glycans in roots, whereas N-glycan processing in leaves depends more heavily on HEXO3 than on HEXO1. Because hexo1 hexo3 double mutants do not display any obvious phenotype even upon exposure to different forms of abiotic or biotic stress, it should be feasible to improve the quality of glycoprotein therapeutics produced in plants by down-regulation of endogenous β-N-acetylhexosaminidase activities. 相似文献
999.
Adler G Steeg C Pfeffer K Murphy TL Murphy KM Langhorne J Jacobs T 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(10):5310-5319
The immune response against the blood stage of malaria has to be tightly regulated to allow for vigorous antiplasmodial activity while restraining potentially lethal immunopathologic damage to the host like cerebral malaria. Coinhibitory cell surface receptors are important modulators of immune activation. B and T lymphocyte attenuator (BTLA) (CD272) is a coinhibitory receptor expressed by most leukocytes, with the highest expression levels on T and B cells, and is involved in the maintenance of peripheral tolerance by dampening the activation of lymphocytes. The function of BTLA is described in several models of inflammatory disorders and autoimmunity, but its function in infectious diseases is less well characterized. Also, little is known about the influence of BTLA on non-T cells. In this study, we analyzed the function of BTLA during blood-stage malaria infection with the nonlethal Plasmodium yoelii strain 17NL. We show that BTLA knockout mice exhibit strongly reduced parasitemia and clear the infection earlier compared with wild-type mice. This increased resistance was seen before the onset of adaptive immune mechanisms and even in the absence of T and B cells but was more pronounced at later time points when activation of T and B cells was observed. We demonstrate that BTLA regulates production of proinflammatory cytokines in a T cell-intrinsic way and B cell intrinsically regulates the production of P. yoelii 17NL-specific Abs. These results indicate that the coinhibitory receptor BTLA plays a critical role during experimental malaria and attenuates the innate as well as the subsequent adaptive immune response. 相似文献
1000.
Pavlovic S Liezmann C Blois SM Joachim R Kruse J Romani N Klapp BF Peters EM 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(2):848-855
Interaction between the nervous and immune systems greatly contributes to inflammatory disease. In organs at the interface between our body and the environment, the sensory neuropeptide substance P (SP) is one key mediator of an acute local stress response through neurogenic inflammation but may also alter cytokine balance and dendritic cell (DC) function. Using a combined murine allergic inflammation/noise stress model with C57BL/6 mice, we show in this paper that SP--released during repeated stress exposure--has the capacity to markedly attenuate inflammation. In particular, repeated stress exposure prior to allergen sensitization increases DC-nerve fiber contacts, enhances DC migration and maturation, alters cytokine balance, and increases levels of IL-2 and T regulatory cell numbers in local lymph nodes and inflamed tissue in a neurokinin 1-SP-receptor (neurokinin-1 receptor)-dependent manner. Concordantly, allergic inflammation is significantly reduced after repeated stress exposure. We conclude that SP/repeated stress prior to immune activation acts protolerogenically and thereby beneficially in inflammation. 相似文献