全文获取类型
收费全文 | 23954篇 |
免费 | 2044篇 |
国内免费 | 11篇 |
出版年
2023年 | 120篇 |
2022年 | 276篇 |
2021年 | 545篇 |
2020年 | 294篇 |
2019年 | 373篇 |
2018年 | 502篇 |
2017年 | 430篇 |
2016年 | 776篇 |
2015年 | 1211篇 |
2014年 | 1369篇 |
2013年 | 1617篇 |
2012年 | 2032篇 |
2011年 | 1927篇 |
2010年 | 1258篇 |
2009年 | 1096篇 |
2008年 | 1522篇 |
2007年 | 1523篇 |
2006年 | 1350篇 |
2005年 | 1277篇 |
2004年 | 1212篇 |
2003年 | 1118篇 |
2002年 | 1044篇 |
2001年 | 249篇 |
2000年 | 170篇 |
1999年 | 191篇 |
1998年 | 265篇 |
1997年 | 171篇 |
1996年 | 165篇 |
1995年 | 159篇 |
1994年 | 134篇 |
1993年 | 120篇 |
1992年 | 103篇 |
1991年 | 89篇 |
1990年 | 84篇 |
1989年 | 76篇 |
1988年 | 70篇 |
1987年 | 66篇 |
1986年 | 62篇 |
1985年 | 63篇 |
1984年 | 67篇 |
1983年 | 71篇 |
1982年 | 57篇 |
1981年 | 46篇 |
1980年 | 61篇 |
1979年 | 37篇 |
1978年 | 59篇 |
1977年 | 54篇 |
1976年 | 45篇 |
1974年 | 45篇 |
1973年 | 34篇 |
排序方式: 共有10000条查询结果,搜索用时 421 毫秒
41.
42.
Donald R. McCarty Christian B. Carson Mark Lazar S. Caprice Simonds 《Genesis (New York, N.Y. : 2000)》1989,10(6):473-481
The viviparous-1 (vp1) locus in maize is a developmental gene that controls diverse aspects of the maturation phase of seed development. Mutations of vp1 alter embryo sensitivity to the hormone abscisic acid and block formation of anthocyanin pigment. Molecular cloning of a Robertson Mutator-induced mutant allele, vp1-mum-1, by transposable element tagging has allowed analysis of several transposon-induced vp1 mutants. In the vp1-Mc mutation, the gene is disrupted by 4.0 kbp insertion, which results in expression of a 3′ truncated mRNA. Phenotypically, this allele is at least partially functional in causing embryo dormancy, but is ineffective in controlling anthocyanin expression. This result suggests that disruption of the C-terminal domain of the Vp1 protein specifically affects regulation of the anthocyanin pathway. A second Mutator- derived allele, vp1-mum2, exhibits an unusual form of somatic mutability in which endosperm cells revert from wild-type vp1 expression to a mutant condition. The vp1-mum2 allele contains a 1.5 kbp Insertion that has no detectable homology to known Mu elements. This element is retained In wild-type germinal revertants derived from vp1-mum2 An apparent DNA modification affecting cleavage at an internal Sstl restriction site in the element correlates with vp1-mum2 states that exhibit wild-type Vp1 expression. A model involving mitotic assortment of modified and unmodified DNA strands during development is proposed for vp1-mum2 somatic mutation. 相似文献
43.
44.
Christian Paech John Pierce Stephen D. McCurry N.E. Tolbert 《Biochemical and biophysical research communications》1978,83(3):1084-1092
Xylulose-1,5-bisphosphate in preparations of ribulose-1,5-bisphosphate (ribulose-P2) arises from non-enzymic epimerization and inhibits the enzyme. Another inhibitor, a diketo degradation product from ribulose-P2, is also present. Both compounds simulate the substrate inhibition of ribulose-P2 carboxylase/oxygenase previously reported for ribulose-P2. Freshly prepared ribulose-P2 had little inhibitory activity. The instability of ribulose-P2 may be one reason for a high level of ribulose-P2 carboxylase in chloroplasts where the molarity of active sites exceeds that of ribulose-P2. Because the KD of the enzyme/substrate complex is ≤1 μM, all ribulose-P2 generated in situ may be stored as this complex to prevent decomposition. 相似文献
45.
Intraspecific variation in reproductive physiology and egg quality in the European Starling Sturnus vulgaris 总被引:1,自引:0,他引:1
Egg mass shows large intraspecific variation in birds and is repeatable within individuals. The mechanisms underlying this variation are unknown. We hypothesized that measures of egg quality (the mass of yolk protein, yolk lipid, and albumen protein) would be positively correlated with the plasma pools of the yolk precursor vitellogenin, and the masses of the oviduct, metabolic machinery (liver, heart, lungs, kidneys, gizzard, small intestine and pancreas), and endogenous stores of protein and lipid. We tested these predictions in European Starlings Sturnus vulgaris collected at the peak of egg production effort. In contrast to our predictions, both yolk protein and yolk lipid were negatively correlated with plasma vitellogenin levels. Albumen protein was positively related to oviduct mass, but other aspects of body composition failed to explain variation in egg quality. Hence, while we observed correlations between egg composition and peripheral systems (circulating precursor pools and the oviduct), we found no evidence that egg quality is determined by more general processes, i.e., the supply and processing of nutrients. 相似文献
46.
47.
48.
49.
Periphytic communities in running waters were examined as they developed on granite rocks, concrete balls and glass slides. At equivalent cell densities, no differences in pigment concentrations, species diversity or production levels were found among the different substrata examined. Development of the assemblage appeared to result from the elongation of short algal filaments which had initially settled on the surface. As these communities matured, a distinct canopy and understory developed. Cellular metabolisms were comparable among the communities. In the understory of the communities, even though the cellular content of chl a and b did not differ, chl c and carotenoid pigment concentrations were higher than those in the over-story. Bicarbonate assimilation of Tabellaria fenestrata (Lyng.) Külz. and Eunotia pectinalisi var. pectinalis (O. F. Müll?) Rabh. was higher than that of the more abundant Tabellaria flocculosa (Roth.)Kütz. var. flocculosa IV (sensu Koppen) at both high and low cell densities. This probably reflects a seasonal succession of colonizing species. Glucose assimilation appeared to be mainly attributable to bacterial activity, and algal cells of the upper layer were less active than those of the bottom. The small amount of glucose that was incorporated by the algal cells was probably absorbed passively since its amount was in direct proportion to cell volumes. 相似文献
50.
A note on the hatching and viability ofCeriodaphnia ephippia collected from lake sediment 总被引:3,自引:3,他引:0
Christian Moritz 《Hydrobiologia》1987,145(1):309-314
Ephippia ofCeriodaphnia pulchella Sars were collected at 2 sites from successive sediment layers.
Hatching observed in the laboratory gave information about the duration of their viability. Conclusions about the hatching
situation in the lake were drawn from the ratio of intact to total ephippia at various lake depths. The results are discussed. 相似文献