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991.
Gijs Teklenburg Madhuri Salker Mariam Molokhia Stuart Lavery Geoffrey Trew Tepchongchit Aojanepong Helen J. Mardon Amali U. Lokugamage Raj Rai Christian Landles Bernard A. J. Roelen Siobhan Quenby Ewart W. Kuijk Annemieke Kavelaars Cobi J. Heijnen Lesley Regan Jan J. Brosens Nick S. Macklon 《PloS one》2010,5(4)
Background
Pregnancy is widely viewed as dependent upon an intimate dialogue, mediated by locally secreted factors between a developmentally competent embryo and a receptive endometrium. Reproductive success in humans is however limited, largely because of the high prevalence of chromosomally abnormal preimplantation embryos. Moreover, the transient period of endometrial receptivity in humans uniquely coincides with differentiation of endometrial stromal cells (ESCs) into highly specialized decidual cells, which in the absence of pregnancy invariably triggers menstruation. The role of cyclic decidualization of the endometrium in the implantation process and the nature of the decidual cytokines and growth factors that mediate the crosstalk with the embryo are unknown.Methodology/Principal Findings
We employed a human co-culture model, consisting of decidualizing ESCs and single hatched blastocysts, to identify the soluble factors involved in implantation. Over the 3-day co-culture period, approximately 75% of embryos arrested whereas the remainder showed normal development. The levels of 14 implantation factors secreted by the stromal cells were determined by multiplex immunoassay. Surprisingly, the presence of a developing embryo had no significant effect on decidual secretions, apart from a modest reduction in IL-5 levels. In contrast, arresting embryos triggered a strong response, characterized by selective inhibition of IL-1β, -6, -10, -17, -18, eotaxin, and HB-EGF secretion. Co-cultures were repeated with undifferentiated ESCs but none of the secreted cytokines were affected by the presence of a developing or arresting embryo.Conclusions
Human ESCs become biosensors of embryo quality upon differentiation into decidual cells. In view of the high incidence of gross chromosomal errors in human preimplantation embryos, cyclic decidualization followed by menstrual shedding may represent a mechanism of natural embryo selection that limits maternal investment in developmentally impaired pregnancies. 相似文献992.
993.
Question: Is it possible to restore dry calcareous inland sand ecosystems with their characteristic plant community structure within a 4‐yr period by means of combined abiotic–biotic techniques (topsoil replacement, inoculation with raked/mown plant material from target areas)? Location: Upper Rhine valley, Germany. Methods: Two 4‐year experiments were carried out on former arable land, each in the proximity of a reference area bearing a similar complex of threatened sandy grasslands (experiment 1: fine‐scale; experiment 2: landscape scale). In both experiments we used nutrient‐poor deep‐sand substrate (abiotic approach), raked/mown inoculation material from target areas and grazing as management tool (biotic and management approach). The vegetation of the restoration and donor areas was sampled once a year and analysed by non‐metric multidimensional scaling (NMDS) ordination and target‐species ratios. Mixed linear models were calculated to determine effects of grazing (experiment 1) and year (both experiments). Results: NMDS revealed a continuous development of the restored sites towards the corresponding donor sites. Similarly, target‐species ratios of the restored sites tended towards the ratios of the donor sites. To date, grazing effects have mainly been structural: reduction of a carpet‐forming pleurocarpous moss species and of litter. In addition, cover of target species in relation to total plant cover was significantly enhanced by grazing in the last two study years. Conclusions: The combination of nutrient‐poor substrate, inoculation with raked/mown plant material and grazing proved to be a very effective restoration method for dry base‐rich sand ecosystems. After 4 yr the restored plant communities serve as well‐developed parts of a habitat network. 相似文献
994.
Wilfried Konrad Christian Apeltauer Jrg Frauendiener Wilhelm Barthlott Anita Roth-Nebelsick 《仿生工程学报(英文版)》2009,6(4):350-356
We describe a few mathematical tools which allow to investigate whether air-water interfaces exist(under prescribedconditions)and are mechanically stable and temporally persistent.In terms of physics,air-water interfaces are governed by theYoung-Laplace equation.Mathematically they are surfaces of constant mean curvature which represent solutions of a nonlinearelliptic partial differential equation.Although explicit solutions of this equation can be obtained only in very special cases,it is-under moderately special circumstances-possible to establish the existence of a solution without actually solving thedifferential equation.We also derive criteria for mechanical stability and temporal persistence of an air layer.Furthermorewe calculate the lifetime of a non-persistent air layer.Finally,we apply these tools to two examples which exhibit the symmetriesof 2D lattices.These examples can be viewed as abstractions of the biological model represented by the aquatic fernSalvinia. 相似文献
995.
About 200 micromammal isolated teeth (rodents, lagomorphs) originating from the middle Orleanian locality of Béon 2 (Montréal-du-Gers, SW France) are described. The rodent fauna is dominated by myomorphs (7 species), including the cricetid Democricetodon aff. hispanicus Fahlbusch, the melissiodontid Melissiodon sp., the glirids Peridyromys murinus (Pomel), Pseudodryomys aff. ibericus, Pseudodryomys aff. simplicidens, and Glirudinus modestus (Dehm), and the eomyid Ligerimys aff. florancei. Sciuromorphs are represented only by Heteroxerus rubricati Crusafont, Villalta and Truyols. Within lagomorphs, 2 dental morphs referred to the ochotonid Prolagus Pomel are identified in Béon 2. They correspond to Prolagus oeningensis (König) and P. aff. vasconiensis. On a biostratigraphical point of view, this study confirms the location of Béon 2 at the early part of the MN4; this locality is older than other regional sites such as Pellecahus, Béon 1, La Romieu, and Bézian (MN4b), and it is coeval to Artenay, in the Loire basin. In particular, glirids and lagomorphs from Béon 2 testify to close relationships with micromammal localities referred to the MN3 biozone. 相似文献
996.
Achim Brockmeier Manuel Skopnik Brigitte Koch Christian Herrmann Stefan Welti Klaus Scheffzek 《Biochemical and biophysical research communications》2009,388(4):630-636
Eubacteria can import and simultaneously phosphorylate a range of different carbohydrates by means of sugar specific phosphoenolpyruvate (PEP) dependent sugar phosphotransferase systems (PTSs). Here, we report the biochemical characterization of the gluconate specific PTS component EIIAgnt from Enterococcus faecalis and its unexpectedly strong complex with EIIBgnt. We analyze the activity of the complex regarding phosphoryl transfer using kinetic measurements and demonstrate by mutagenesis that His-9 of EIIAgnt is essential for this process and represents most likely the phosphoryl group carrier of EIIAgnt. With a combination of isothermal titration calorimetry (ITC), analytical ultracentrifugation (AUC), native gel electrophoresis and chemical crosslinking experiments we show that EIIAgnt and EIIBgnt form a strong 2:2 heterotetrameric complex, which seems to be destabilized upon phosphorylation of EIIBgnt. 相似文献
997.
Melissa A. Edeling S. Kyle Austin Bimmi Shrestha Kimberly A. Dowd Swati Mukherjee Christopher A. Nelson Syd Johnson Manu N. Mabila Elizabeth A. Christian Joseph Rucker Theodore C. Pierson Michael S. Diamond Daved H. Fremont 《PLoS pathogens》2014,10(4)
We recently described our most potently neutralizing monoclonal antibody, E106, which protected against lethal Dengue virus type 1 (DENV-1) infection in mice. To further understand its functional properties, we determined the crystal structure of E106 Fab in complex with domain III (DIII) of DENV-1 envelope (E) protein to 2.45 Å resolution. Analysis of the complex revealed a small antibody-antigen interface with the epitope on DIII composed of nine residues along the lateral ridge and A-strand regions. Despite strong virus neutralizing activity of E106 IgG at picomolar concentrations, E106 Fab exhibited a ∼20,000-fold decrease in virus neutralization and bound isolated DIII, E, or viral particles with only a micromolar monovalent affinity. In comparison, E106 IgG bound DENV-1 virions with nanomolar avidity. The E106 epitope appears readily accessible on virions, as neutralization was largely temperature-independent. Collectively, our data suggest that E106 neutralizes DENV-1 infection through bivalent engagement of adjacent DIII subunits on a single virion. The isolation of anti-flavivirus antibodies that require bivalent binding to inhibit infection efficiently may be a rare event due to the unique icosahedral arrangement of envelope proteins on the virion surface. 相似文献
998.
Marion Koch Nathana?l Delmotte Christian H. Ahrens Ulrich Omasits Kathrin Schneider Francesco Danza Barnali Padhi Valérie Murset Olivier Braissant Julia A. Vorholt Hauke Hennecke Gabriella Pessi 《Applied and environmental microbiology》2014,80(7):2094-2101
Rhizobia have a versatile catabolism that allows them to compete successfully with other microorganisms for nutrients in the soil and in the rhizosphere of their respective host plants. In this study, Bradyrhizobium japonicum USDA 110 was found to be able to utilize oxalate as the sole carbon source. A proteome analysis of cells grown in minimal medium containing arabinose suggested that oxalate oxidation extends the arabinose degradation branch via glycolaldehyde. A mutant of the key pathway genes oxc (for oxalyl-coenzyme A decarboxylase) and frc (for formyl-coenzyme A transferase) was constructed and shown to be (i) impaired in growth on arabinose and (ii) unable to grow on oxalate. Oxalate was detected in roots and, at elevated levels, in root nodules of four different B. japonicum host plants. Mixed-inoculation experiments with wild-type and oxc-frc mutant cells revealed that oxalotrophy might be a beneficial trait of B. japonicum at some stage during legume root nodule colonization. 相似文献
999.
Esther Nkuipou-Kenfack Flore Duranton Nathalie Gayrard àngel Argilés Ulrika Lundin Klaus M. Weinberger Mohammed Dakna Christian Delles William Mullen Holger Husi Julie Klein Thomas Koeck Petra Zürbig Harald Mischak 《PloS one》2014,9(5)
Chronic kidney disease (CKD) is part of a number of systemic and renal diseases and may reach epidemic proportions over the next decade. Efforts have been made to improve diagnosis and management of CKD. We hypothesised that combining metabolomic and proteomic approaches could generate a more systemic and complete view of the disease mechanisms. To test this approach, we examined samples from a cohort of 49 patients representing different stages of CKD. Urine samples were analysed for proteomic changes using capillary electrophoresis-mass spectrometry and urine and plasma samples for metabolomic changes using different mass spectrometry-based techniques. The training set included 20 CKD patients selected according to their estimated glomerular filtration rate (eGFR) at mild (59.9±16.5 mL/min/1.73 m2; n = 10) or advanced (8.9±4.5 mL/min/1.73 m2; n = 10) CKD and the remaining 29 patients left for the test set. We identified a panel of 76 statistically significant metabolites and peptides that correlated with CKD in the training set. We combined these biomarkers in different classifiers and then performed correlation analyses with eGFR at baseline and follow-up after 2.8±0.8 years in the test set. A solely plasma metabolite biomarker-based classifier significantly correlated with the loss of kidney function in the test set at baseline and follow-up (ρ = −0.8031; p<0.0001 and ρ = −0.6009; p = 0.0019, respectively). Similarly, a urinary metabolite biomarker-based classifier did reveal significant association to kidney function (ρ = −0.6557; p = 0.0001 and ρ = −0.6574; p = 0.0005). A classifier utilising 46 identified urinary peptide biomarkers performed statistically equivalent to the urinary and plasma metabolite classifier (ρ = −0.7752; p<0.0001 and ρ = −0.8400; p<0.0001). The combination of both urinary proteomic and urinary and plasma metabolic biomarkers did not improve the correlation with eGFR. In conclusion, we found excellent association of plasma and urinary metabolites and urinary peptides with kidney function, and disease progression, but no added value in combining the different biomarkers data. 相似文献
1000.