Effect of Phosphate on the Corrosion of Carbon Steel and on the Composition of Corrosion Products in Two-Stage Continuous Cultures of Desulfovibrio desulfuricans

A field isolate of Desulfovibrio desulfuricans was grown in defined medium in a two-stage continuous culture apparatus with different concentrations of phosphate in the feed medium. The first state (V1) was operated as a conventional chemostat (D = 0.045 h⁻¹) that was limited in energy source (lactate) or phosphate. The second stage (V2) received effluent from V1 but no additional nutrients, and contained a healthy population of transiently starved or resting cells. An increase in the concentration of phosphate in the medium fed to V1 resulted in increased corrosion rates of carbon steel in both V1 and V2. Despite the more rapid corrosion observed in growing cultures relative to that in resting cultures, corrosion products that were isolated under strictly anaerobic conditions from the two culture modes had similar bulk compositions which varied with the phosphate content of the medium. Crystalline mackinawite (Fe₉S₈), vivianite [Fe₃(PO₄)₂ · 8H₂O], and goethite [FeO(OH)] were detected in amounts which varied with the culture conditions. Chemical analyses indicated that the S in the corrosion product was almost exclusively in the form of sulfides, while the P was present both as phosphate and as unidentified components, possibly reduced P species. Some differential localization of S and P was observed in intact corrosion products. Cells from lactate-limited, but not from phosphate-limited, cultures contained intracellular granules that were enriched in P and Fe. The results are discussed in terms of several proposed mechanisms of microbiologically influenced corrosion.     

The effect of sodium chlorate and nitrapyrin on the nitrification mediated nitrosation process in soils

Summary The influence of total nitrification to nitrate or partial nitrification to nitrite on the soil organic nitrogen status was examined. NH ₄ ⁺ –¹⁵N was added to the soil in the absence and the presence of NaClO₃, respectively nitrapyrin. The first chemical inhibits only nitrate formation, the second inhibits total nitrification. The accumulation of nitrite nitrogen in the soil at levels up to 5 mg kg^–1 increased the loss of nitrogen. Yet, it did not increase the binding of mineral nitrogen into soil organic matter, relative to the control soil. The data suggest that the biochemistry of the nitrite formation process, rather than the levels of nitrite ions formed, are of primary importance in the role of nitrification mediated nitrosation of soil organic matter.     

Dynamics and management of some coastal dune woodlands near The Hague,The Netherlands

Changes are reported of Crataegus-Betula dune woodlands between 1950–1980 from Meijendel, a calcareous coastal dune system near the city of The Hague, The Netherlands, which is used as a water catchment area. It concerns both woodlands in degradation stage and, more common, woodlands on the increase. Changes were recorded with help of successive vegetation maps, air photos and permanent plot observations. Woodland increase usually occurs on places with groundwater at or near the surface, either because of natural circumstances or as a result of artificial groundwater recharge through infiltration as part of the water catchment activities. The management of both types of woodland is discussed.Nomenclature follows Heukels-van der Meijden (1983), Flora van Nederland, 20th ed. Wolters-Noordhoff, Groningen. Nomenclature of mosses follows Margadant & During (1982), Beknopte Flora van Nederlandse Blad- en Levermossen, Thieme, Zutphen.The authors have pleasure in thanking their colleagues from the Dune Water Works who supplied data on hydrology.     

M-MuLV-induced leukemogenesis: Integration and structure of recombinant proviruses in tumors

M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral sequences. All reintegrated genomes carry long terminal repeat (LTR) sequences at both termini of their genome. In the preleukemic stage a large population of cells exhibiting a random distribution of reintegrated M-MuLV genomes are seen, but during outgrowth of the tumor, selection of cells occurs leaving one or a few clonal descendants in the outgrown tumor. In this latter stage recombinant genomes can be detected. Although these recombinants constitute a heterogeneous group of proviruses, characteristic molecular markers are conserved among many individual proviral recombinants, lending credence to the notion that a certain recombinant structure is a prerequisite for the onset of neoplasia. The structure of these recombinants shows close structural similarities to the previously described mink cell focus-inducing (MCF)-type viruses.     

An unsupervised neural network model for the development of reflex co-ordination

In this paper, we present a model for the development of connections between muscle afferents and motoneurones in the human spinal cord. The model consists of a limb with six muscles, one motoneurone pool, one pooled (Ia-like) afferent for each muscle and a central programme generator. The weights of the connections between the afferents and the motoneurone pools are adapted during centrally induced movements of the limb. The connections between the afferents and the motoneurone pools adapt in a hebbian way, using only local information present at the synapses. This neural network is tested in two examples of a limb with two degrees of freedom and six muscles. Despite the simplifications, the model predicts the pattern of autogenic and heterogenic monosynaptic reflexes quite realistically.     

Temperature Response of Early Foliar Expansion of Potato and Wheat

We studied the course of early leaf area expansion and specificleaf area (S_LA) in potato (Solanum tuberosum L.) and wheat (Triticumaestivum L.) genotypes and tested whether air temperature explainsdifferences in these courses within different environments.Such knowledge can be used to improve crop growth modelling.The relative rate of leaf area expansion (R_L) of potato andwheat decreased with thermal time, but was nearly linear upto a leaf area index (L) of 1.0. TheR_L (L < 1; mean: 17.9x 10^-3°C^-1 d^-1) of potato showed an interaction betweengenotype and environment, and varied with year. TheR_L (L <1; mean: 7.1 x 10^-3°C^-1 d^-1) of winter wheat was lower thanthat of spring wheat (mean: 10.9 x 10^-3°C^-1 d^-1), and bothvaried with year. S_LAof potato increased nearly linearly withthermal time from 5 to 15 m² kg^-1at 50% emergence, to 20 to25 m² kg^-1at 155°Cd, and then decreased slightly. The S_LAofboth winter and spring wheat began at 16 to 23 m² kg^-1and inmost cases increased slightly with thermal time. In potato,regression parameters of S_LAwith thermal time were affectedby environment (management conditions and year) and genotype;in wheat they were affected by environment (year and site).Treatment effects on R_Lof potato were not correlated with thoseon S_LA , and were only partly correlated for wheat. Thereforewe conclude that the early foliar expansion of potato is associatedwith a strong increase in S_LA , and not so for wheat. For bothcrops the course of early leaf area expansion and ofS_LA withair temperature is not robust over environments and genotypes.The consequences of these results for modelling are discussed.Copyright 2000 Annals of Botany Company Triticum aestivum, spring wheat, winter wheat, Solanum tuberosum, leaf area expansion, specific leaf area, early growth, genotype, environment, modelling