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排序方式: 共有147条查询结果,搜索用时 15 毫秒
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Susan L. Makris Howard M. Solomon Ruth Clark Kohei Shiota Stephane Barbellion Jochen Buschmann Makoto Ema Michio Fujiwara Konstanze Grote Keith P. Hazelden Kok Wah Hew Masao Horimoto Yojiro Ooshima Meg Parkinson L. David Wise 《Birth defects research. Part B, Developmental and reproductive toxicology》2011,92(6):575-575
146.
Protein interaction with ice. 总被引:3,自引:0,他引:3
Many organisms have evolved novel mechanisms to minimize freezing injury due to extracellular ice formation. This article reviews our present knowledge on the structure and mode of action of two types of proteins capable of ice interaction. The antifreeze proteins inhibit ice crystal formation and alter ice growth habits. The ice nucleation proteins, on the other hand, provide a proper template to stimulate ice growth. The potential applications of these proteins in different industries are discussed. 相似文献
147.
The successful expression and purification of the recombinant ocean pout antifreeze polypeptide (rAFP) in Escherichia coli have enabled the study of its structure-function relationship by site-directed mutagenesis. The role of carboxyl groups at Glu23 and Glu36 of the rAFP was probed by replacing these residues with either glutamine or alanine residues as both single and double mutants. The AFP mutants were expressed, purified and characterized in terms of primary and secondary structures, thermal stability and antifreeze activities. The properties of these mutants were compared with those of the rAFP. Three distinct functions are identified for the carboxyl groups: (i) the negative charges at positions 23 and 36 are involved in the thermal stability of the polypeptide; (ii) the negative charges at positions 23 and 36 contribute to the thermal hysteretic activities of the polypeptide; and (iii) the negative charge at position 23 and hydrogen-bonding ability at position 36 contribute to the ice-binding activity of the polypeptide. 相似文献