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121.
The effects of adrenocorticotropic hormones on murine CGI-105 gene expression were investigated in 3T3-L1 cells. Expression was markedly increased in differentiated cells and it was up-regulated 2-fold in cells induced to differentiate with dexamethasone. 相似文献
122.
Adenoviral proteins mimic nutrient/growth signals to activate the mTOR pathway for viral replication 总被引:4,自引:0,他引:4
O'Shea C Klupsch K Choi S Bagus B Soria C Shen J McCormick F Stokoe D 《The EMBO journal》2005,24(6):1211-1221
Like tumor cells, DNA viruses have had to evolve mechanisms that uncouple cellular replication from the many intra- and extracellular factors that normally control it. Here we show that adenovirus encodes two proteins that activate the mammalian target of rapamycin (mTOR) for viral replication, even under nutrient/growth factor-limiting conditions. E4-ORF1 mimics growth factor signaling by activating PI3-kinase, resulting in increased Rheb.GTP loading and mTOR activation. E4-ORF4 is redundant with glucose in stimulating mTOR, does not affect Rheb.GTP levels and is the major mechanism whereby adenovirus activates mTOR in quiescent primary cells. We demonstrate that mTOR is activated through a mechanism that is dependent on the E4-ORF4 protein phosphatase 2A-binding domain. We also show that mTOR activation is required for efficient S-phase entry, independently of E2F activation, in adenovirus-infected quiescent primary cells. These data reveal that adenovirus has evolved proteins that activate the mTOR pathway, irrespective of the cellular microenvironment, and which play a requisite role in viral replication. 相似文献
123.
Jiwoon Kim Keemin Park Minsung Kim Hyungjun Lee Junghyun Choi Ho Bum Park Hansu Kim Jaeyoung Jang Young-Hoon Kim Taeseup Song Ungyu Paik 《Liver Transplantation》2024,14(10):2303455
Roll-to-roll dry processing enables the manufacture of high energy density and low cost Li-ion batteries (LIBs). However, as the thickness of the electrode fabricated by dry processing becomes greater (≥10 mAh cm−2), Li-ion migration resistance (Rion) and charge-transfer resistance (Rct) in the electrode dramatically increase due to long diffusion lengths for Li-ion and electron. Therefore, it is important to reduce diffusion lengths in the electrode to achieve high energy density LIBs. The dry electrode with a high areal capacity of 10 mAh cm−2 and low resistance can be achieved by following three characteristics. First, the fibrillization behavior of polytetrafluoroethylene (PTFE) binder is controlled by adjusting the processing temperature during the fibrillization process, which enables uniform distribution of PTFE binder and carbon black (CB). Second, pore size/distribution and conducting network are engineered by multi-dimensional conducting agents, enhancing Li-ions and electrons transport in the electrode. Finally, the structural integrity of LiNi0.80Co0.15Al0.05O2 (NCA) particles is improved without fractures, which enables uniform pore distribution in the electrode by controlling the calendering step. The prepared 10 mAh cm−2 dry electrode with homogeneous microstructure shows reduced Rion and Rct due to short diffusion lengths, which improves electrochemical performances in LIBs with a high volumetric energy density of ≈710 Wh L−1. 相似文献
124.
SUMMARY: TO-GO is a Gene Ontology (GO) navigation tool, which is implemented as a Java application. After the initial data downloading, the GO term tree can be interactively navigated without further network transfer. Local annotation can be incorporated. It supports querying by GO terms or associated gene product information, displaying the result as a table or a sub-tree. The result from the search for a set of external database accessions includes the number of gene products associated with each node, inclusive of sub-nodes. Search results can be further processed by set operations and these set operations can be quite useful for expression profile data analysis. A copy/paste function is also implemented in order to facilitate data exchange between applications. AVAILABILITY: TO-GO is freely available at http://www.ngic.re.kr/togo/index.html CONTACT: ungsik@kribb.re.kr 相似文献
125.
So Yung Yang Ji Hyun Baek Youngah Cho Eun-Young Cho Yujin Choi Yongkang Kim 《Chronobiology international》2018,35(3):405-415
ST8SIA2 and NCAM1 are functionally related genes forming polysialic acid (PSA) - neural cell adhesion molecule (NCAM) complex in suprachiasmatic nucleus (SCN), the regulating site of circadian biological rhythm. In this study, the relationship of ST8SIA2 and NCAM1 with circadian and seasonal rhythms of human behavior was explored. Subjects were 261 healthy Korean adults who were free of any history of clinically significant psychiatric symptoms. The phenotypes were circadian preference and seasonal change of mood and behavior (seasonality) measured by the Composite Scale of Morningness and the Seasonal Pattern Assessment Questionnaire, respectively. Thirty-four single nucleotide polymorphisms (SNPs) across the ST8SIA2 region and 15 SNPs of NCAM1 were analyzed. A nominally significant association with seasonality and circadian preference was observed in 21 variants of both genes. After corrections for multiple testing, associations of 8 SNPs of ST8SIA2 and 2 SNPs of NCAM1 with seasonality remained significant. Some of these SNPs were also associated with psychiatric disorders in previous studies. This study demonstrated a meaningful and/or suggestive evidence of association between behavioral phenotypes reflecting human biological rhythm and two interplaying genes involved in the plasticity of SCN’s neuronal network. 相似文献
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129.
Jung Won Shin Sung Hee Choi Dong Eun Kim Hee Sook Kim Jae-Hwa Lee In Su Lee Eun Yeol Lee 《Bioprocess and biosystems engineering》2011,34(1):113-119
The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an
Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading
activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic
iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared
by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation. 相似文献
130.