全文获取类型
收费全文 | 628篇 |
免费 | 44篇 |
出版年
2022年 | 5篇 |
2021年 | 3篇 |
2019年 | 7篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 15篇 |
2014年 | 29篇 |
2013年 | 44篇 |
2012年 | 31篇 |
2011年 | 39篇 |
2010年 | 18篇 |
2009年 | 19篇 |
2008年 | 24篇 |
2007年 | 37篇 |
2006年 | 26篇 |
2005年 | 32篇 |
2004年 | 36篇 |
2003年 | 18篇 |
2002年 | 27篇 |
2001年 | 23篇 |
2000年 | 18篇 |
1999年 | 20篇 |
1998年 | 12篇 |
1997年 | 7篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 14篇 |
1992年 | 15篇 |
1991年 | 15篇 |
1990年 | 8篇 |
1989年 | 13篇 |
1988年 | 8篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 2篇 |
1979年 | 2篇 |
1976年 | 2篇 |
1975年 | 6篇 |
1974年 | 2篇 |
1973年 | 5篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1963年 | 2篇 |
排序方式: 共有672条查询结果,搜索用时 15 毫秒
21.
Endothelin-3 stimulates production of endothelium-derived nitric oxide via phosphoinositide breakdown. 总被引:7,自引:0,他引:7
T Emori Y Hirata K Kanno K Ohta S Eguchi T Imai M Shichiri F Marumo 《Biochemical and biophysical research communications》1991,174(1):228-235
Cultured bovine endothelial cells (EC) have specific receptors for endothelin (ET)-3 functionally coupled to phosphoinositide breakdown. We studied whether ET-3 stimulates synthesis of nitric oxide (NO), an endothelium-derived relaxing factor that activates soluble guanylate cyclase in EC, and whether the ET-3-induced NO formation involves G-proteins. ET-3 dose-dependently stimulated production of intracellular cGMP in EC, of which effects were abolished by pretreatment with NG-monomethyl L-arginine, an inhibitor of NO synthesis, and methylene blue, an inhibitor of soluble guanylate cyclase. The stimulatory effects of ET-3 on cGMP production, inositol trisphosphate formation and increase in cytosolic free Ca2+ concentration were similarly blocked by pretreatment with pertussis toxin (PTX). These data suggest that ET-3 induces synthesis of NO mediated by phosphoinositide breakdown via PTX-sensitive G-protein in EC. 相似文献
22.
K Ohta Y Hirata T Imai K Kanno T Emori M Shichiri F Marumo 《Biochemical and biophysical research communications》1990,169(2):578-584
To elucidate the mechanism by which endothelin-1 (ET-1) is released from renal epithelial cells, we have investigated the effects of several compounds on release of ET-1-like immunoreactivity (LI) from LLCPK1 cell line. Thrombin, transforming growth factor-beta, cytokines (tumor necrotizing factor-alpha, interleukin-1 beta), and phorbol ester stimulated ET-1-LI release in a time- and dose-dependent manner. The cytokine-induced ET-1-LI release was not affected by indomethacin. Northern blot analysis using cDNA for porcine preproET-1 as a probe revealed a single major band corresponding to the size of ET-1 mRNA in LLCPK1. These data indicate that the preproET-1 gene is also expressed in renal epithelial cells and the release of ET-1 from renal cells is regulated by the similar mechanism to that from endothelial cells. 相似文献
23.
24.
25.
Cell cycle-dependent expression of antigen-binding and I-J-bearing molecules on suppressor T cell hybridomas 总被引:1,自引:0,他引:1
K Takabayashi N Suzuki M Kanno K Imai T Tokuhisa H Tomioka M Taniguchi 《Journal of immunology (Baltimore, Md. : 1950)》1983,130(6):2552-2556
The I-J and antigen-binding chains with constant region determinant (Ct) that compose an antigen-specific suppressor T cell factor were found on the surface of suppressor T cell hybridomas, serologically and morphologically demonstrated by a fluorescence-activated cell sorter (FACS) and immunoelectron microscopic analyses. Moreover, the surface expression of the I-J and Ct-bearing chains fluctuating with the same kinetics depended entirely upon the cell cycle. The maximum expression of these two chains was observed in the early stage of the M phase, and the minimum in the S phase. Similarly, the magnitude of the suppressor activity was maximal in the late stage of the M phase, and was minimal in the S phase. The results therefore demonstrated that there exists good correlation between the cell surface expression of the I-J and Ct-bearing chains and the magnitude of the suppressor activity produced. The antigen recognition units on suppressor T cell hybridomas have serologically and morphologically been characterized by using radiolabeled antigens or monoclonal antibodies against the I-J or Ct on the antigen-binding molecule. Cell-binding assay and radioautographic analysis demonstrated that the suppressor T cell hybridoma possesses the capacity to bind native antigen in an antigen-specific fashion as does the hybridoma-derived, antigen-specific suppressor factor composed of the I-J and the Ct-bearing chains, indicating that the recognition unit on the cell surface is composed of a structure similar to the factor. 相似文献
26.
Junzou Hiratsuka Hiroaki Shimada Robert Whittier Takashi Ishibashi Masahiro Sakamoto Masao Mori Chihiro Kondo Yasuko Honji Chong-Rong Sun Bing-Yuan Meng Yu-Qing Li Akira Kanno Yoko Nishizawa Atsushi Hirai Kazuo Shinozaki Masahiro Sugiura 《Molecular & general genetics : MGG》1989,217(2-3):185-194
Summary The entire chloroplast genome of the monocot rice (Oryza sativa) has been sequenced and comprises 134525 bp. Predicted genes have been identified along with open reading frames (ORFs) conserved
between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (Nicotiana tabacum), and a liverwort (Marchantia polymorpha). The same complement of 30 tRNA and 4 rRNA genes has been conserved between rice and tobacco. Most ORFs extensively conserved
betweenN. tabacum andM. polymorpha are also conserved intact in rice. However, several such ORFs are entirely absent in rice, or present only in severely truncated
form. Structural changes are also apparent in the genome relative to tobacco. The inverted repeats, characteristic of chloroplast
genome structure, have expanded outward to include several genes present only once per genome in tobacco and liverwort and
the large single copy region has undergone a series of inversions which predate the divergence of the cereals. A chimeric
tRNA pseudogene overlaps an apparent endpoint of the largest inversion, and a model invoking illegitimate recombination between
tRNA genes is proposed which accounts simultaneously for the origin of this pseudogene, the large inversion and the creation
of repeated sequences near the inversion endpoints. 相似文献
27.
28.
29.
Kanno Yosuke Shu En Niwa Hirofumi Seishima Mariko Ozaki Kei-ichi 《Molecular biology reports》2021,48(4):3431-3437
Molecular Biology Reports - Systemic sclerosis (SSc) is characterized by peripheral circulatory disturbance and fibrosis in skin and visceral organs. We recently demonstrated that... 相似文献
30.
Reactive oxygen species (ROS) released from polymorphonuclear leukocytes and macrophages could cause DNA damage, but also induce cell death. Therefore inhibition of cell death must be an important issue for accumulation of genetic changes in lymphoid cells in inflammatory foci. Scavengers in the post culture medium of four lymphoid cell lines, lymphoblastoid cell lines (LCL), Raji, BJAB and Jurkat cells, were examined. Over 80% of cultured cells showed cell death 24 h after xanthine (X)/xanthine oxidase (XOD) treatment, which was suppressed by addition of post culture medium from four cell lines in a dose-dependent manner. H2O2 but not O·-2 produced by the X/XOD reaction was responsible for the cytotoxity, thus we used H2O2 as ROS stress thereafter. The H2O2-scavenging activity of post culture media from four cell lines increased rapidly at the first day and continued to increase in the following 2–3 days for LCL, Raji and BJAB cells. The scavenging substance was shown to be pyruvate, with various concentrations in the cultured medium among cell lines. Over 99% of total pyruvate was present in the extracellular media and less than 1% in cells. α-Cyano-4-hydroxycinnamate, a specific inhibitor of the H+-monocarbohydrate transporter, increased the H2O2-scavenging activity in the media from all four cell lines via inhibition of pyruvate re-uptake by cultured cells from the media. These findings suggest that lymphoid cells in inflammatory foci could survive even under ROS by producing pyruvate, so that accumulation of lymphoid cells with DNA damage is possible. 相似文献