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91.
Crown ethers are toxic to Escherichia coli. At a sublethal dosage, the crown ether affects the three phases in the bacterial growth curve as evidenced by an appearance of a lag period, an occasional decrease in the stationary phase at a lower microbial population. Potassium ion but not sodium ion can reduce the lag induced by the presence of 18-crown-6. On the contrary, the presence of either potassium ion or sodium ion lengthens the lag due to substituted 18-crown-6 ethers. Explanations to this variable toxicity are proposed. 相似文献
92.
Ornithine decarboxylase (ODC) activity increases by 2 times in the process of progesterone-induced Bufo oocyte maturation (Table 1). Tumor promotor phorbol ester (PMA) is unable to affect both basal and stimulated ODC activity (Fig. 5) although it is capable of elevating the rate of steroid-induced maturation (Fig. 4). Spermine can inhibit significantly ODC activity of oocytes (Fig. 3). Hormone-stimulated ODC activity falls by 17% when Bufo oocytes are cultured in the alkaline Ringer's solution containing 5 mM spermine (pH 11.6) (Fig. 2). The period, however, is shortened by more than 50% during which the oocytes undergo GVBD (Fig. 1). Otherwise, spermine is found to repress ODC activity in dose dependent manner when microinjected in Bufo oocytes (Fig. 3). But oocytes undergo GVBD with a frequency of more than 80% when progesterone-induced increment of the enzyme activity is totally inhibited in the oocytes injected with approximately 50 nl 4.0 mM spermine. The conclusion may emerge from the above-stated results that increased ornithine decarboxylase activity is not essential for progesterone-induced Bufo oocyte maturation. In addition, ODC activity begins to increase rapidly when endogenous spermine level has been lowered to the largest extent in the maturation process. Therefore the endogenous spermine probably acts as a physiologically negative regulator of ODC activity since exogenous spermine inhibits seriously ODC activity of Bufo oocytes. 相似文献
93.
The mechanism of photosynthetic water oxidation in spinach was investigated with a newly developed inhibitor of the water-oxidizing complex, acetone hydrazone (AceH), (CH3)2CNNH2 [Tso, J., Petrouleas, V., & Dismukes, G.C. (1990) Biochemistry (preceding paper in this issue)], by using fluorescence induction and single-turnover flashes to monitor O2 yield and thermoluminescence intensity. AceH binds slowly (1-3 min) in the dark to the S1 (resting) oxidation state of the water-oxidizing complex in thylakoids and PSII membranes. Once bound, it causes a two-flash delay in the pattern of O2 release seen in a train of flashes. This is initiated by reduction of manganese in the S2 oxidation state of the complex in a fast reaction (less than 0.5 s). In thylakoid membranes which have been partially inhibited at low AceH concentrations (less than 2 mM) the inhibition can be reversed by a single flash and a subsequent dark period. This behavior can be explained by two sequential one-electron oxidation steps: S1.AceHhv----S2.AceH in equilibrium S1.AceH+hv----S2.AceH+----S1 + AceH2+ Dissociation of the unobserved radical intermediate, AceH+, from S1 is proposed to account for the recovery from inhibition after one flash. In contrast, recovery from inhibition after a single flash is not observed in detergent-isolated PSII membranes or in intact thylakoid membranes at higher AceH concentrations (greater than 2 mM), where the two-flash delay in O2 release is seen. This suggests either a concerted two-electron process, S2----S0, or tight binding of AceH+ to S1.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
94.
Formation of a bispecific antibody by the use of leucine zippers. 总被引:10,自引:0,他引:10
A new method is described for the production of bispecific F(ab')2 heterodimers using leucine zippers. Two heterodimer-forming "zipper" peptides derived from the Fos and Jun proteins were respectively linked to the Fab' portions of two different mAb by gene fusion. The antibodies used were 145-2C11, which binds to murine CD3, and anti-Tac, which binds to the p55 chain of the human IL-2R. Anti-Tac Fab'-Jun and anti-CD3 Fab'-Fos were expressed individually as F(ab'-zipper)2 homodimers in the mouse myeloma cell line Sp2/0. When these homodimers were reduced at the hinge region to form monomers and then reoxidized together, the resulting end products were mostly F(ab'-zipper)2 heterodimers. Bispecific anti-CD3 x anti-Tac F(ab'-zipper)2 heterodimers produced by this method were shown to be highly effective in recruiting cytotoxic T cells to lyse IL-2R-bearing HuT-102 cells in vitro. 相似文献
95.
The pineal body and the retina of the neonatal Sprague-Dawley rat were studied by light and electron microscopy, and the morphologic differentiation of the parenchymal cells of the pineal body was compared with that of the developing photoreceptor cells of the retina. Between the ages of 4 and 12 days after birth, some of the developing pinealocytes were observed to become elongated and polarized, with their nuclei located at one pole. "Synaptic" ribbons were observed within the cell body. At the opposite pole the cells developed elongated cell processes that initially contained microtubules and ribosomes. These cell processes projected into luminal spaces and were attached by structures resembling zonulae adherentes to the adjacent cells. Extending from the tips of the cell processes, cilia with a 9 + 0 arrangement were observed. Lamellated and vesicular membranes were noted at the tips of the cilia. Such morphologic differentiation, however, could be observed only in rats younger than 17 days. Comparison of the morphologic features of the neonatal pinealocytes with those of the developing retinal photoreceptor cells showed much similarity. It is suggested that the pinealocytes of the neonatal rat undergo "photoreceptor-like" differentiation during a transient neonatal period. Such morphologic differentiation may provide an explanation for light-induced biochemical changes described in neonatal rats whose eyes had been enucleated. 相似文献
96.
People with the variants of glucose-6-phosphate dehydrogenase (GPD) deficiency common in the southern Chinese (Canton, B(-)Chinese, and Hong Kong-Pokfulam) have a moderate shortening of red-cell survival but no anaemia when they are in the steady state. With a cross-transfusion technique, primaquine, nitrofurantoin, and large doses of aspirin were found to aggravate the haemolysis while sulphamethoxazole did so only in some people. Individual differences in drug metabolism may be the reason for this. Many commonly used drugs reported to accentuate haemolysis in GPD deficiency did not shorten red-cell survival. 相似文献
97.
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. 总被引:153,自引:8,他引:145
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Full length cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rat and man have been isolated and sequenced. Many GAPDH gene-related sequences have been found in both genomes based on genomic blot hybridization analysis. Only one functional gene product is known. Results from genomic library screenings suggest that there are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome. Some of these related sequences have been shown to be processed pseudogenes. We have isolated several rat cDNA clones corresponding to these pseudogenes indicating that some pseudogenes are transcribed. Rat and human cDNAs are 89% homologous in the coding region, and 76% homologous in the first 100 base pairs of the 3'-noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophila and yeast genes allows the analysis of the evolution of the GAPDH genes in detail. 相似文献
98.
Y M Choy W W Tso K P Fung K C Leung Y F Tsang C Y Lee D Tsang 《Biochemical and biophysical research communications》1978,82(1):305-309
Auricular electroacupuncture (AES) has been found to be successful in the suppression of withdrawal symptoms of morphine-addicted mice. In abrupt withdrawals precipited by naloxone, the plasma adrenocorticotropin (ACTH) rises to a high level which can also be effectively suppressed by AES. This elevation of plasma ACTH is not due to naloxone, as naloxone has no effect on the ACTH level in non-addicted mice. The possible physiological effect produced by AES is discussed. 相似文献
99.
X-linked adrenoleukodystrophy (XALD, MIM 300100), the commonest inherited peroxisomal disorder, is characterized by central nervous system demyelination, primary adrenal failure and the systemic accumulation of saturated very long chain fatty acids (VLCFAs). The defective gene ABCD1 encodes an ATP-binding cassette (ABC) transport protein named ALDP, which functions as a crucial transporter of VLCFAs into the peroxisomes for beta-oxidation. Here, we report a Chinese man with adrenomyeloneuropathy characterized by Addison's disease and spastic paraparesis. His plasma VLCFA levels, ratios of C24:0/C22:0 and C26:0/C22:0 were all significantly elevated. We performed mutation analysis of the ABCD1 gene in the proband and the family members using direct DNA sequencing and restriction analysis. A novel insertion 496_497insG in exon 1 causing a frame shift and a premature stop codon at amino acid position 194 (D194X) was identified (GenBank accession No. NM_000033). The insertional mutation abolishes an HhaI restriction site. The same mutation was found in his mother and the eldest sister even though their clinical and biochemical abnormalities were milder. Diagnosis of XALD often relies upon the detection of elevated VLCFA levels and ratios of C26:0/C22:0 and C24:0/C22:0 in fasting blood, however, 5-15% of the obligate heterozygotes would give normal values. DNA-based testing thus remains the most reliable tool for heterozygote detection when the disease-causing mutations are known. Using restriction fragment length polymorphism with HhaI, we have devised a rapid method for the identification of the carriers among the proband's family members and possibly for the screening of the mutations in other XALD patients. 相似文献
100.