Effect of a streptococcal allergen on the rosette-forming capacity of the T-lymphocytes in erysipelas patients

The reaction of antigen-dependent E-rosette formation with the hemolytic streptococcal antigen in erysipelas patients is indicative of the ambiguous role of the specific immunological transformation of the body in respect of the infective agent antigens in different clinical forms of the disease and is of prognostic importance as regards the chronic transformation of the infectious process and the development of the relapses of the disease.     

Effect of a hypertonic sodium chloride solution on dye distribution in the organs and lymph of the thoracic duct]

The distribution of trephan blue in different organs and tissues and in the lymph was studied in acute experiments on 84 dogs after the infusion of 5% NaCl solution (0.5 mg/kg). The rise of the stain concentration in the intact dogs was the greatest in the liver, the kidney and the intestine. The osmotic shift caused by the intravenous infusion of the hypertonic NaCl solution increased the blood plasma hyaluronidase activity, reduced the maximal accumulation time of the stain in the tissues and led to a 1.5-2 fold elevation in the stain concentration in the organs.     

Pleiotropic aspartate taxis and serine taxis mutants of Escherichia coli.

Mutants that at one time were thought to be specifically defective in taxis toward aspartate and related amino acids (tar mutants) or specifically defective in taxis toward serine and related amino acids (tar mutants) are now shown to be pleiotropic in their defects. The tar mutants also lack taxis toward maltose and away from Co2+ and Ni2+. The tsr mutants are altered in their response to a variety of repellents. Double mutants (tar tsr) fail in nearly all chemotactic responses. The tar and tsr mutants provide evidence for two complementary, converging pathways of information flow: certain chemoreceptors feed information into the tar pathway and others into the tsr pathway. The tar and tsr products have been shown to be two different sets of methylated proteins.     

Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions

Maturation promoting factor (MPF) activity was recovered from progesterone-matured Xenopus oocytes cytosol, fractionated by polyethylene glycol-20,000 or ethanolic precipitation. An improved stabilization of the biological activity was obtained by adding adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) (50 microM) to the preparation buffers. Neither Ca2+ ions nor calmodulin inhibit the partially purified MPF.     

Transport kinetics for superoxide dismutase and catalase between plasma and interstitial fluid in the rat small intestine

The purpose of these studies was to determine the initial rates (first 5 h) of plasma-to-interstitial fluid transport for superoxide dismutase, catalase, and albumin in the rat small intestine. In all experiments, the renal vascular pedicles were ligated to prevent the renal excretion of these macromolecules. Plasma and intestinal interstitial fluid (lymph) samples were collected at timed intervals after bolus intravenous administration of SOD, catalase, or 125I-labeled albumin. Before injection of the proteins, the plasma concentrations (43.8 +/- 16.9 and 7.6 +/- 1.2 U/mL, respectively), interstitial fluid (lymph) concentrations (28.8 +/- 7.6 and 1.6 +/- 0.8 U/mL, respectively), and the lymph-to-plasma (L/P) protein concentration ratios (0.59 +/- 0.13 and 0.22 +/- 0.09, respectively) for endogenous SOD and catalase were determined. The plasma disappearance rate for exogenously administered catalase far exceeded the rates for SOD or albumin. However, the rate of catalase disappearance from the plasma was markedly reduced in animals in which the circulation through the liver was eliminated, suggesting that the hepatic route may be important for elimination of exogenously administered catalase. Maximal interstitial fluid catalase concentrations were achieved within 30 min while SOD and albumin required 45-90 min. The L/P ratios for exogenously administered SOD and albumin increased to 0.22 +/- 0.06 and 0.19 +/- 0.03 within 60 and 120 min of injection, respectively, and remained at these levels for the remainder of the experimental protocol. The catalase L/P ratio increased to 0.24 +/- 0.07 within 90 min of injection and subsequently declined to levels measured for endogenous catalase over the remaining 3.5 h.(ABSTRACT TRUNCATED AT 250 WORDS)