全文获取类型
收费全文 | 181篇 |
免费 | 16篇 |
国内免费 | 1篇 |
出版年
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 1篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 8篇 |
2015年 | 11篇 |
2014年 | 10篇 |
2013年 | 14篇 |
2012年 | 15篇 |
2011年 | 14篇 |
2010年 | 13篇 |
2009年 | 9篇 |
2008年 | 10篇 |
2007年 | 9篇 |
2006年 | 4篇 |
2005年 | 4篇 |
2004年 | 2篇 |
2003年 | 4篇 |
2002年 | 1篇 |
2001年 | 6篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 10篇 |
1997年 | 6篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有198条查询结果,搜索用时 15 毫秒
171.
The cellulolytic fungus Trichoderma reesei QM9414 was cultivated on spent brewery grains to partially hydrolyse the grains into soluble sugars and to alter the amino acid profile of the substrate. Aflatoxins were not detected in either the treated or the untreated spent grains. Amino acid profiles indicated that both the untreated and partially hydrolysed spent grains possess the entire range of essential amino acids required by poultry. Treated grains contained higher levels of lysine, histidine, glycine, methionine, phenylalanine, proline and alanine compared to those in untreated grains. The incorporation of treated grains at 8% and 12% into starter rations for broiler chickens in the first 4 weeks resulted in significant improvement in their growth and feed conversion ratio. There was no significant difference in feed conversion after 6 and 8 weeks of growth. 相似文献
172.
173.
174.
The chaetognaths, or arrowworms, constitute a small and enigmatic phylum of
marine invertebrates whose phylogenetic affinities have long been
uncertain. A popular hypothesis is that the chaetognaths are the sister
group of the major deuterostome phyla: chordates, hemichordates, and
echinoderms. Here we attempt to determine the affinities of the
chaetognaths by using molecular sequence data. We describe the isolation
and nucleotide sequence determination of 18S ribosomal DNA from one species
of chaetognath and one acanthocephalan. Extensive phylogenetic analyses
employing a suite of phylogenetic reconstruction methods (maximum
parsimony, maximum likelihood, evolutionary parsimony, and two distance
methods) suggest that the hypothesized relationship between chaetognaths
and the deuterostomes is incorrect. In contrast, we propose that the
lineage leading to the chaetognaths arose prior to the advent of the
coelomate metazoa.
相似文献
175.
176.
A phylogenetic survey using the polymerase chain reaction (PCR) has
identified four major P element subfamilies in the saltans and willistoni
species groups of Drosophila. One subfamily, containing about half of the
sequences studied, consists of elements that are very similar to the
canonical (and active) P element from D. melanogaster. Within this
subfamily, nucleotide sequence differentiation among different copies from
the same species and among elements from different species is relatively
low. This observation suggests that the canonical elements are relatively
recent additions to the genome or, less likely, are evolving slowly
relative to the other subfamilies. Elements belonging to the three
noncanonical lineages are distinct from the canonical elements and from one
another. Furthermore, there is considerably more sequence variation, on the
average, within the noncanonical subfamilies compared to the canonical
elements. Horizontal transfer and the coexistence of multiple,
independently evolving element subfamilies in the same genome may explain
the distribution of P elements in the saltans and willistoni species
groups. Such explanations are not mutually exclusive, and each may be
involved to varying degrees in the maintenance of P elements in natural
populations of Drosophila.
相似文献
177.
Femke AH van der Linden Jolijn J Kragt Margarethe van Bon Martin Klein Alan J Thompson Henk M van der Ploeg Chris H Polman Bernard MJ Uitdehaag 《BMC neurology》2008,8(1):2
Background
The use of self-report measurements in clinical settings is increasing. However, in patients with limitations that interfere with reliable self-assessment such as cognitive impairment or mood disturbances, as may be the case in multiple sclerosis (MS), data collection might be problematic. In these situations, information obtained from proxy respondents (e.g. partners) may replace self-ratings. The aim of this study was to examine the value of proxy ratings at separate points in time and to assess patient-proxy agreement on possible changes in disease impact of MS. 相似文献178.
Towards a reporter system to identify regulators of cross-talk between salicylate and jasmonate signaling pathways in Arabidopsis 总被引:1,自引:0,他引:1
Annemart Koornneef Adriaan Verhage Antonio Leon-Reyes Reinier Snetselaar LC Van Loon Corné MJ Pieterse 《Plant signaling & behavior》2008,3(8):543-546
The plant signaling hormones salicylic acid (SA) and jasmonic acid (JA) are regulators of inducible defenses that are activated upon pathogen or insect attack. Cross-talk between SA- and JA-dependent signaling pathways allows a plant to finely tune its response to the attacker encountered. In Arabidopsis, pharmacological experiments revealed that SA exerts a strong antagonistic effect on JA-responsive genes, such as PDF1.2, indicating that the SA pathway can be prioritized over the JA pathway. SA-mediated suppression of the JA-responsive PDF1.2 promoter was exploited for setting up a genetic screen aiming at the isolation of signal transduction mutants that are impaired in this cross-talk mechanism. The PDF1.2 promoter was fused to the herbicide resistance gene BAR to allow for life/death screening of a population of mutagenized transgenic plants. Non-mutant plants should survive herbicide treatment when methyl jasmonate (MeJA) is applied, but suppression of the JA response by SA should be lethal in combination with the herbicide. Conversely, crucial SA/JA cross-talk mutants should survive the combination treatment. SA effectively suppressed the expression of the PDF1.2::BAR transgene. However, suppression of the BAR gene did not result in suppression of herbicide resistance. Hence, a screening method based on quantitative differences in the expression of a reporter gene may be better suited to identify SA/JA cross-talk mutants. Here, we demonstrate that the PDF1.2::GUS reporter will be excellently suited in this respect.Key words: plant defense, salicylic acid, jasmonic acid, cross-talk, mutant screen, Arabidopsis 相似文献
179.
180.
卢志南 孙兴国 Songshou Mao Matthew J. Budoff William W. Stringer 葛万刚 李浩 黄洁 刘方 胡盛寿 《中国应用生理学杂志》2015,31(4):332-336
目的: 当前评估左心室容量和功能仍常用正常值范围,个体化分析也仅使用体表面积进行校正。尚缺少个体化因素相关的大样本参考值和预计公式。方法: 本研究纳入美国加州洛杉矶县南湾地区1200名健康志愿者,其中男807女393,年龄20岁-94岁,心脏CT造影(CTA),经过高精度三维成像技术处理,计算左心室容积在收缩和舒张过程中的连续动态变化,测定左心室(LV)容量和功能指标:舒张末期容积(EDV)、收缩末期容积(ESV)、每搏输出量(SV)、射血分数(EF)和心输出量(CO)。将以上指标与一般特征指标进行多因素相关分析,以探索正常人预计值计算公式。结果: 男性除LVEF小于女性外(P<0.001),其余各指标均大于女性(P<0.001)。多元线性回归分析提示, 性别、年龄、身高和体质量均为EDV、ESV、SV的独立影响因子(P<0.001); 而CO仅受年龄、性别、体质量显著影响(P<0.001),但与身高无关(P>0.05)。CO的预测公式CO (L·min-1)= 6.963+0.446(Male) -0.037×年龄(yr)+0.013×体质量(kg)。结论: 性别、年龄、身高、体质量均影响左心室容量和功能,建立预测值计算公式,对心血管疾病的无创评估和个体化精准医疗具有重要参考价值。 相似文献