Extreme cost of rivalry in a monandrous species: male–male interactions result in failure to acquire mates and reduced longevity

Mating system variation is profound in animals. In insects, female willingness to remate varies from mating with hundreds of males (extreme polyandry) to never remating (monandry). This variation in female behaviour is predicted to affect the pattern of selection on males, with intense pre-copulatory sexual selection under monandry compared to a mix of pre- and post-copulatory forces affecting fitness under polyandry. We tested the hypothesis that differences in female mating biology would be reflected in different costs of pre-copulatory competition between males. We observed that exposure to rival males early in life was highly costly for males of a monandrous species, but had lower costs in the polyandrous species. Males from the monandrous species housed with competitors showed reduced ability to obtain a mate and decreased longevity. These effects were specific to exposure to rivals compared with other types of social interactions (heterospecific male and mated female) and were either absent or weaker in males of the polyandrous species. We conclude that males in monandrous species suffer severe physiological costs from interactions with rivals and note the significance of male–male interactions as a source of stress in laboratory culture.     

Latest Pleistocene—Holocene paleoceanographic trends on the continental margin of eastern Canada: Foraminiferal,dinoflagellate and pollen evidence

Micropaleontological studies were made of cores from four shelf basins on the eastern Canadian Margin: Emerald and Canso basins on the Scotian Shelf (44°–46° N), Notre Dame Channel, Newfoundland Shelf (50° N) and Cartwright Saddle, Labrador Shelf (55°). Events were correlated using a combination of¹⁴C dates and pollen stratigraphies. Surface- and bottom-water changes were compared on the basis of dinoflagellates and benthic foraminifera, respectively. The results indicate significant paleoceanographic shifts along a north—south gradient both prior to and during the Holocene.Distinct Late Pleistocene—Holocene paleoceanographic events were distinguished in the Emerald, Canso and Notre Dame basins; these events are less obvious in Cartwright Saddle which is in deeper water and further off-shore. Pleistocene glaciomarine sediments in all basins contain a fauna dominated byElphidium excavatum f.clavata; dinoflagellates and pollen are rare or absent. The widespreadElphidium fauna probably reflects turbid glacial meltwater and/or a permanent ice shelf cover from 20,000-10,000 yrs BP. The Notre Dame core also penetrates older sediment with an outer Labrador Current fauna which may represent a late Wisconsinian interstade at about 23,000 yrs BP. From 7,000–10,000 yrs BP a cold water fauna occurred which is similar to modern outer Labrador Current faunas. From about 5000–7000 yrs BP, a warm interval is indicated by a relatively warm-water calcareous benthonic foraminiferal fauna and increased representation of typical Gulf Stream dinoflagellates. The most recent change occurred in the last 2000 years with an abrupt cooling associated with stronger flow of the arctic inner Labrador Current. This cooling event is marked by an increase in arctic dinoflagellates and by an exclusively agglutinated benthonic foraminiferal fauna at two sites (Canso and Notre Dame). These Holocene paleoceanographic changes are not clearly seen in the benthic fauna of the deep northern basin (Cartwright Saddle) although dinoflagellate data at this site indicate that surface-water changes have occurred that are similar to those found in shallower basins.Shifts in the zonal position of the Gulf Stream and changes in the relative mass transports of the West Greenland and Labrador currents are mechanisms which may account for the paleoceanographic events. The glacial—interstadial—glacial sequence recorded in the Notre Dame Channel, in conjunction with other theories on glacial triggering mechanisms, provides biostratigraphic evidence which suggests the onset of a glacial stage in the near future.     

The structure of the human adenovirus 2 penton

The adenovirus penton, a noncovalent complex of the pentameric penton base and trimeric fiber proteins, comprises the vertices of the adenovirus capsid and contains all necessary components for viral attachment and internalization. The 3.3 A resolution crystal structure of human adenovirus 2 (hAd2) penton base shows that the monomer has a basal jellyroll domain and a distal irregular domain formed by two long insertions, a similar topology to the adenovirus hexon. The Arg-Gly-Asp (RGD) motif, required for interactions with cellular integrins, occurs on a flexible surface loop. The complex of penton base with bound N-terminal fiber peptide, determined at 3.5 A resolution, shows that the universal fiber motif FNPVYPY binds at the interface of adjacent penton base monomers and results in a localized structural rearrangement in the insertion domain of the penton base. These results give insight into the structure and assembly of the adenovirus capsid and will be of use for gene-therapy applications.     

Crystal structures of alfalfa caffeoyl coenzyme A 3-O-methyltransferase

Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are S-adenosyl-l-methionine-dependent O-methyltransferases (OMTs) involved in lignin biosynthesis. Plant CCoAOMTs belong to a distinct family of OMTs, more closely related to the mammalian catechol OMTs than to other plant OMTs. The crystal structure of alfalfa (Medicago sativa) CCoAOMT in complex with the reaction products S-adenosine-l-homocysteine and feruloyl/sinapoyl CoAs presented here belong to a structurally and mechanistically distinct family of plant small molecule OMTs. These structures provide a new understanding of the substrate preferences and the catalytic mechanism accompanying CCoAOMT-mediated O-methylation of CoA-linked phenylpropanoid substrates.     

Memory Effects on Movement Behavior in Animal Foraging

An individual’s choices are shaped by its experience, a fundamental property of behavior important to understanding complex processes. Learning and memory are observed across many taxa and can drive behaviors, including foraging behavior. To explore the conditions under which memory provides an advantage, we present a continuous-space, continuous-time model of animal movement that incorporates learning and memory. Using simulation models, we evaluate the benefit memory provides across several types of landscapes with variable-quality resources and compare the memory model within a nested hierarchy of simpler models (behavioral switching and random walk). We find that memory almost always leads to improved foraging success, but that this effect is most marked in landscapes containing sparse, contiguous patches of high-value resources that regenerate relatively fast and are located in an otherwise devoid landscape. In these cases, there is a large payoff for finding a resource patch, due to size, value, or locational difficulty. While memory-informed search is difficult to differentiate from other factors using solely movement data, our results suggest that disproportionate spatial use of higher value areas, higher consumption rates, and consumption variability all point to memory influencing the movement direction of animals in certain ecosystems.     

Insights into mechanisms of graft-versus-host disease through humanised mouse models

Graft-versus-host disease (GVHD) is a major complication that occurs following allogeneic haematopoietic stem cell transplantation (HSCT) for the treatment of haematological cancers and other blood-related disorders. GVHD is an inflammatory disorder, where the transplanted donor immune cells can mediate an immune response against the recipient and attack host tissues. Despite over 60 years of research, broad-range immune suppression is still used to prevent or treat GVHD, leading to an increased risk of cancer relapse and infection. Therefore, further insights into the disease mechanisms and development of predictive and prognostic biomarkers are key to improving outcomes and reducing GVHD development following allogeneic HSCT. An important preclinical tool to examine the pathophysiology of GVHD and to understand the key mechanisms that lead to GVHD development are preclinical humanised mouse models. Such models of GVHD are now well-established and can provide valuable insights into disease development. This review will focus on models where human peripheral blood mononuclear cells are injected into immune-deficient non-obese diabetic (NOD)-scid-interleukin-2(IL-2)Rγ mutant (NOD-scid-IL2Rγ^null) mice. Humanised mouse models of GVHD can mimic the clinical setting for GVHD development, with disease progression and tissues impacted like that observed in humans. This review will highlight key findings from preclinical humanised mouse models regarding the role of donor human immune cells, the function of cytokines and cell signalling molecules and their impact on specific target tissues and GVHD development. Further, specific therapeutic strategies tested in these preclinical models reveal key molecular pathways important in reducing the burden of GVHD following allogeneic HSCT.     

Nrl‐Cre transgenic mouse mediates loxP recombination in developing rod photoreceptors

The developing mouse retina is a tractable model for studying neurogenesis and differentiation. Although transgenic Cre mouse lines exist to mediate conditional genetic manipulations in developing mouse retinas, none of them act specifically in early developing rods. For conditional genetic manipulations of developing retinas, a Nrl‐Cre mouse line in which the Nrl promoter drives expression of Cre in rod precursors was created. The results showed that Nrl‐Cre expression was specific to the retina where it drives rod‐specific recombination with a temporal pattern similar to endogenous Nrl expression during retinal development. This Nrl‐Cre transgene does not negatively impact retinal structure and function. Taken together, the data suggested that the Nrl‐Cre mouse line was a valuable tool to drive Cre‐mediated recombination specifically in developing rods. genesis 54:129–135, 2016. © 2016 Wiley Periodicals, Inc.