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排序方式: 共有366条查询结果,搜索用时 31 毫秒
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We examined the effects of prostaglandin E (PGE) receptor subtype EP4 antagonist on bone metastasis of cancer to clarify PGE's role in bone metastasis. Metastatic regions were detected in femurs accompanying severe bone loss in mice injected with B16 malignant melanoma cells. Administration of EP4 antagonist restored the bone loss induced by B16 melanoma. Adding B16 cells induced osteoclast formation in the coculture of bone marrow cells and osteoblasts without any exogenous bone-resorbing factor, and EP4 antagonist completely suppressed the osteoclast formation induced by B16 cells. Therefore, EP4 antagonist is a possible candidate for the therapy of bone metastasis of cancer. 相似文献
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Induction of apoptosis by epigallocatechin-3-gallate in human lymphoblastoid B cells 总被引:1,自引:0,他引:1
Noda C He J Takano T Tanaka C Kondo T Tohyama K Yamamura H Tohyama Y 《Biochemical and biophysical research communications》2007,362(4):951-957
(-)-Epigallocatechin-3-gallate (EGCG), a major constituent of green tea polyphenols, has been shown to suppress cancer cell proliferation and induce apoptosis. In this study we investigated its efficacy and the mechanism underlying its effect using human B lymphoblastoid cell line Ramos, and effect of co-treatment with EGCG and a chemotherapeutic agent on apoptotic cell death. EGCG induced dose- and time-dependent apoptotic cell death accompanied by loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, and cleavage of pro-caspase-9 to its active form. EGCG also enhanced production of intracellular reactive oxygen species (ROS). Pretreatment with diphenylene iodonium chloride, an inhibitor of NAD(P)H oxidase and an antioxidant, partially suppressed both EGCG-induced apoptosis and production of ROS, implying that oxidative stress is involved in the apoptotic response. Furthermore, we showed that combined-treatment with EGCG and a chemotherapeutic agent, etoposide, synergistically induced apoptosis in Ramos cells. 相似文献
125.
Tanabe H Ayabe T Maemoto A Ishikawa C Inaba Y Sato R Moriichi K Okamoto K Watari J Kono T Ashida T Kohgo Y 《Biochemical and biophysical research communications》2007,358(1):349-355
alpha-Defensin is an antimicrobial peptide which plays an important role in innate immunity. Human defensin (HD)-5 is stored in the Paneth cells of the small intestine as a pro-form and is cleaved by trypsin, which is co-secreted from the Paneth cell granules. The mature HD-5 is protected from further digestion by the proteolysis enzyme. We generated both recombinant HD-5 and proHD-5, and the reduced form of each peptide in order to determine their physiological roles of the disulfide bonds. The reduced proHD-5 attenuated the bactericidal activity and the stability against the trypsin digestion. Human defensin was protected from the enzymatic degradation by disulfide bridges. We further purified the HD-5 with a disulfide variation in the small intestine of Crohn's disease patients. The HD-5 was sensitive to the trypsin treatment. These observations evidently predict that a defensin deficiency may be caused by a disulfide disorder in the disease. 相似文献
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Yoshikazu Sato Chisato Kamiishi Tomoaki Tokaji Masayuki Mori Sanae Koizumi Kyoko Kobayashi Tetsuji Itoh Waka Sonohara Mayura B. Takada Tsuyoshi Urata 《Acta theriologica》2014,59(1):129-137
Tree rubbing by brown bears (Ursus arctos) is a well-known behavior throughout the animal’s distribution. There is still insufficient information on the characteristics and function of the behavior. We investigated seasonal frequency of tree rubbing by brown bears, characteristics and reasons for selection of rub trees, and characteristics of bear signs on and around rub trees in a mixed coniferous–broad-leaved deciduous forest in Hokkaido, Japan. Between 1998 and 2009, we found 172 rub trees and confirmed 995 tree rubbings. We found that the rub trees were used repeatedly by bears within a year and for multiple years (more than 10 years). Tree rubbing by brown bears was observed from April to November, with a peak between May and June that corresponds to the mating season of brown bears. Abies sachalinensis was selected and broad-leaved trees were avoided for tree rubbing based on estimated availability in natural forest. The preference for Abies sachalinensis might be because the strong odor of resin attracts bears for rubbing their head and neck in resinous substances and for increasing the detectability of their markings by receptor bears. Selected trees for rubbing were located right beside the trail on relatively level ground among trees along roads or trails. Trees had a relatively large diameter at breast height. These characteristics would also serve to increase the access and detectability of their markings. Series of pad-shaped depressions was the most frequently observed (70 %) indication of bear rubbing, followed by debarking (51 %) and clawing (30 %). In terms of visual and olfactory signal amplification, physical damage by bears to the trees might have a function to enhance the smell as a result of increase in outflow of the resin. We conclude that tree rubbing behavior is associated with the mating season of brown bears and that the main purpose of this behavior is scent marking to communicate intraspecifically during the mating season. 相似文献
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Shunkei Enoki Akinori Shimizu Chisato Hayashi Hirotake Imanishi Osamu Hashizume Kazuyuki Mekada Hitoshi Suzuki Tetsuo Hashimoto Kazuto Nakada Jun-Ichi Hayashi 《Experimental Animals》2014,63(1):21-30
Previous reports have shown that transmitochondrial mito-mice with nuclear DNA from
Mus musculus and mtDNA from M. spretus do not express
respiration defects, whereas those with mtDNA from Rattus norvegicus
cannot be generated from ES cybrids with mtDNA from R. norvegicus due to
inducing significant respiration defects and resultant losing multipotency. Here, we
isolated transmitochondrial cybrids with mtDNA from various rodent species classified
between M. spretus and R. norvegicus, and compared the
O2 consumption rates. The results showed a strong negative correlation
between phylogenetic distance and reduction of O2 consumption rates, which
would be due to the coevolution of nuclear and mitochondrial genomes and the resultant
incompatibility between the nuclear genome from M. musculus and the
mitochondrial genome from the other rodent species. These observations suggested that
M. caroli was an appropriate mtDNA donor to generate transmitochondrial
mito-mice with nuclear DNA from M. musculus. Then, we generated ES
cybrids with M. caroli mtDNA, and found that these ES cybrids expressed
respiration defects without losing multipotency and can be used to generate
transmitochondrial mito-mice expressing mitochondrial disorders. 相似文献