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101.
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103.
Yuichiro Kida Chisato Kume Maki Hirano Masao Sakaguchi 《Molecular biology of the cell》2010,21(3):418-429
In biogenesis of membrane proteins on the endoplasmic reticulum, a protein-conducting channel called the translocon functions in both the membrane translocation of lumenal domains and the integration of transmembrane segments. Here we analyzed the environments of polypeptide chains during the processes by water-dependent alkylation of N-ethylmaleimide at site-directed Cys residues. Using the technique, the region embedded in the hydrophobic portion of the membrane within a signal-anchor sequence and its shortening by insertion of a Pro residue could be detected. When translocation of the N-terminal domain of the signal-anchor was arrested by trapping an N-terminally fused affinity tag sequence, the signal-anchor was susceptible to alkylation, indicating that its migration into the hydrophobic environment was also arrested. Furthermore, when the tag sequence was separated from the signal-anchor by insertion of a hydrophilic sequence, the signal-anchor became inaccessible to alkylation even in the N-terminally trapped state. This suggests that membrane integration of the signal-anchor synchronizes with partial translocation of its N-terminal domain. Additionally, in an integration intermediate of a membrane protein, both of the two translocation-arrested hydrophilic chains were in an aqueous environment flanking the translocon, suggesting that the translocon provides the hydrophilic pathway capable of at least two translocating chains. 相似文献
104.
Suzuki J Kato A Maeda N Hashimoto C Uchikoshi M Mizutani T Doke C Matsuzawa T 《Primates; journal of primatology》2003,44(3):273-280
We examined growth changes in concentrations of plasma insulin-like growth factor-1 (IGF-1) and testosterone, and somatometric
parameters in two captive male agile gibbons from birth to about 4 years of age, to examine the evolution of growth patterns
in primates. Plasma IGF-1 concentrations in agile gibbons generally increased with age with values ranging from 200 to 1,100 ng/ml.
The growth profiles in plasma IGF-1 in the gibbons were similar to those reported for chimpanzees. The highest concentrations
of plasma testosterone (230 and 296 ng/dl) were observed within the first 0.3 years from birth, then the concentrations rapidly
decreased and fluctuated below 100 ng/dl. Continuously higher IGF-1 concentrations were observed after 2.6 and 3.5 years of
age. The profiles of plasma testosterone in these gibbons also resembled those of other primates including humans. However,
their plasma testosterone levels in both neonate and adult stages (60 ng/dl) were lower than those reported for macaques and
chimpanzees of respective stages. The obtained growth profiles of plasma IGF-1 and testosterone suggest that the adolescent
phase starts around 2.6 or 3.5 years of age in male agile gibbons. The growth trend in many morphological parameters including
body weight showed a linear increase without a significant growth spurt at approximately the onset of puberty. Head length
and first digit length had reached a plateau during the study period. Brachial index, which indicates the relative length
of forearm to upper arm, significantly increased gradually through the growth period. This result indicates that forearm becomes
relatively longer than the upper arm with growth, which may be an evolutionary adaptation for brachiation. 相似文献
105.
Koyama H Nagao TA Inai T Miyahara K Hayasida Y Shirahige K Tsuchiya E 《Bioscience, biotechnology, and biochemistry》2004,68(4):909-919
RSC is a nucleosome-remodeling complex of Saccharomyces cerevisiae essential for growth that can alter histone-DNA interaction by using the energy of ATP hydrolysis. Nps1p/Sth1p is an ATPase subunit of RSC. A mutation in the conserved ATPase domain of Nps1p causes a sporulation defect with decreased expression of early meiotic genes, especially IME2. This defect is partially suppressed by the overexpression of either IME1 or IME2. A homozygous diploid of a novel temperature-sensitive nps1 mutation, nps1-13, harboring amino acid substitutions within the bromodomain, was unable to sporulate. Overexpression of IME, IME2, or both of these genes allowed the completion of meiosis I and meiosis II in nps1-13 but not the formation of mature asci. In nps1-13 carrying YEpIME1, the expression of a group of sporulation-specific genes, which express at the middle stages of sporulation and are required for spore-wall formation, notably diminished, and several late sporulation genes expressed at the early stages of sporulation. These results suggest that Nps1p/RSC plays important roles during the spore development process by controlling gene expression for initiating both meiosis and spore morphogenesis, and ensures proper expression timing of late meiotic genes. 相似文献
106.
Formation of tight junction strands by expression of claudin-1 mutants in their ZO-1 binding site in MDCK cells 总被引:4,自引:4,他引:0
To investigate the formation mechanism of tight junctions (TJs), we constructed three claudin-1 mutants which varied in their COOH-termini and expressed them in MDCK cells under the control of doxycycline. The differences between these constructs are that a putative ZO-1 binding sequence (KDYV) at the COOH-terminus of claudin-1 was deleted (DeltaCmyc) or present (1CLmyc and DeltaCmycYV), or that a myc-epitope was added at the COOH-terminus (1CLmyc and DeltaCmyc) or inserted just before the KDYV sequence (DeltaCmycYV). All three constructs caused the formation of aberrant TJ strands along the lateral plasma membranes. However, when their expression levels were reduced by adding 0.2 ng/ml doxycycline, they were located at apical TJs and colocalized with ZO-1, even in the KDYV-deleted construct. These results suggest that, although the addition of the myc-epitope at or near the COOH-terminus of claudin-1 interfered with the binding to ZO-1 and induced aberrant TJ strand formation, endogenous claudins which could bind to ZO-1 might recruit these deformed claudin-1s expressed at a low level to apical TJs. A calcium switch assay revealed that claudin-1 was transported to cadherin-based cell-cell contacts where ZO-1 had already accumulated, and was then concentrated at apical TJs together with ZO-1. Crosslinking between claudin-1 and the perijunctional actin ring through ZO-1 may be necessary for TJ strands to be localized or retained at apical TJs. 相似文献
107.
Mizutani C Tohyama Y Miura Y Hishita T Nishihara T Yamamura H Ichiyama S Uchiyama T Tohyama K 《Biochemical and biophysical research communications》2002,297(3):664-671
A human megakaryoblastic cell line, CMK, was treated with 12-o-tetradecanoylphorbol-13-acetate (TPA) for differentiation-induction. We examined TPA-induced activation of the MEK1-ERK1/2 pathway in the 100,000g Triton X-insoluble fraction of CMK cells as the membrane skeleton and researched the relation of the MEK1-ERK1/2 activation with integrin expression. We found that this activation was divided into two phases: the first activation occurred transiently in the membrane skeleton fraction of the suspended cell status and diminished after 1h; and the second sustained activation was maintained by cell adhesion. TPA-treated CMK cells revealed increased expression of integrins alphaIIb and beta3 only when the cell adhesion persisted, regardless of the difference of culture substratum. Sustained activation of the MEK1-ERK1/2 pathway is generated in the membrane skeleton by continuous cell adhesion and seems to be essential to TPA-induced megakaryocytic differentiation of CMK cells. 相似文献
108.
Z-Dehydrophenylalanine (ΔzPhe) possessing four oligopeptides, Boc-(L -Ala-ΔzPhe-Aib)n-OCH3 (n = 1–4: Boc, t-butoxycarbonyl; Aib, α-aminoisobutyric acid), were synthesized, and their solution conformations were investigated by 1H-nmr, ir, uv, and CD spectroscopy and theoretical CD calculation. 1H-nmr (the solvent accessibility of NH groups) and ir studies indicated that all the NH groups except for those belonging to the N-terminal L -Ala-ΔzPhe moiety participate in intramolecular hydrogen bonding in chloroform. This suggests that the peptides n = 2–4 have a 4 → 1 hydrogen-bonding pattern characteristic of 310-helical structures. The uv spectra of all these peptides recorded in chloroform and in trimethyl phosphate showed an intense maximum around 276 nm assigned to the ΔzPhe chromophores. The corresponding CD spectra of the peptides n = 2–4 showed exciton couplets with a negative peak at longer wavelengths, whereas that of the peptide n = 1 showed only weak signals. Theoretical CD spectra were calculated for the peptides n = 2–4 of several helical conformations, on the basis of exciton chirality method. This calculation indicated that the three peptides form a helical conformation deviating from the perfect 310-helix that contains three residues per turn, and that their side chains of Δz Phe residues are arranged regularly along the helix. The center-to-center distance between the nearest phenyl pair(s) was estimated to be ~ 5.5 Å. The chemical shifts of the ΔzPhe side-chain protons (Hβ and aromatic H) for the peptides n = 2–4 indicated anisotropic shielding effect of neighboring phenyl group(s); the effect also supports a regular arrangement of the Δz Phe side chains along the helical axis. © 1993 John Wiley & Sons, Inc. 相似文献
109.
Y Fukumoto S Inai K Nagaki K Iida E Yamagami Y Masuho T Watanabe 《Journal of biochemistry》1977,82(4):955-960
S-sulfonated human IgG (S-sIgG) was prepared by treating IgG with sodium sulfite and sodium tetrathionate. The treatment resulted in the selective cleavage of interchain disulfide bonds of the IgG to give S-sulfonate groups. Complement fixing activities of aggregated S-sIgG and the immune complex formed with the S-sIgG antibody were very weak. S-sIgG at a high dose reduced the activity of the first complement component (C1) in normal human serum without any reduction of other complement components activites, but S-alkylated IgG at the same dose did not. Loss of C1 activity was not caused by either S-sulfonated myeloma proteins (IgA and IgE) or urea-treated S-sIgG, in which both inter- and intra-chain disulfide bonds were cleaved. These results suggest that the selective reduction of C1 by S-sIgG is due to a conformational change of the immunoglobulin. 相似文献
110.
Yamanaka A Imai H Adachi M Komatsu M Islam AT Kodama I Kitazawa C Endo K 《Zoological science》2004,21(10):1049-1055
Diapause pupae of Papilio xuthus show color polymorphism, represented by diapause-green, orange, and brownish-orange types that are each associated with specific pupation sites. We investigated the role of the site of pupation on the induction of the development of orange types (or brownish-orange types), and the endocrine mechanism underlying the control of color polymorphism in short-day pupae. All short-day larvae of the wandering stage developed into orange or brownish-orange type pupae when they were placed in rough-surfaced containers after gut-purge. Utilizing a pharate pupal ligation between the thorax and abdomen, the endocrine mechanism underlying the control of color polymorphism was shown to involve a head-thorax factor (Orange-Pupa-Inducing Factor: OPIF) that induced orange types in short-day pupae. OPIF was bioassayed using the ligated abdomens of short-day pharate pupae. OPIF was extractable with 2% NaCl solution from 5th-instar larval ganglia complexes following the mesothoracic complex (TG(2,3)-AG(1-7)), but it could not be extracted with either acetone or 80% ethanol solution. OPIF may not exist in the brains of day-0 pupae or in brain-subesophageal ganglion and prothoracic ganglion complexes of 5th-instar larvae. The short-day pharate pupae responded to OPIF in a dose-dependent manner. 相似文献