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11.
Karpenko LI Ivanisenko VA Pika IA Chikaev NA Eroshkin AM Veremeiko TA Ilyichev AA 《Amino acids》2000,18(4):329-337
Summary. Hepatitis B core antigen is one of the most promising protein carriers of foreign epitopes of various human and animal pathogens.
Chimeric HBcAg particles can be used as effective artificial immunogenes. Unfortunately, not all chimeric proteins are able
to be particulated. The dependence of correct or incorrect folding of chimeric proteins on physical and chemical properties
of inserts was studied with the help of ProAnalyst, SALIX and QSARPro computer programs. We have found that insertion of amino
acids with high hydrophobicity, large volume, and high β-strand index prevent self-assembling chimeric proteins. These factors are most important for the C-termini of inserts. Recommendations
for obtaining correct folding of chimeric HBcAg particles have been given.
Received August 8, 1999, Accepted September 26, 1999 相似文献
12.
Soil inorganic carbon storage pattern in China 总被引:1,自引:0,他引:1
NA MI SHAOQIANG WANG JIYUAN LIU GUIRUI YU WENJUAN ZHANG ESTEBAN JOBBÁGY 《Global Change Biology》2008,14(10):2380-2387
Soils with pedogenic carbonate cover about 30% (3.44 × 106 km2) of China, mainly across its arid and semiarid regions in the Northwest. Based on the second national soil survey (1979–1992), total soil inorganic carbon (SIC) storage in China was estimated to be 53.3±6.3 PgC (1 Pg=1015 g) to the depth investigated to 2 m. Soil inorganic carbon storages were 4.6, 10.6, 11.1, and 20.8 Pg for the depth ranges of 0–0.1, 0.1–0.3, 0.3–0.5, and 0.5–1 m, respectively. Stocks for 0.1, 0.3, 0.5, and 1 m of depth accounted for 8.7%, 28.7%, 49.6%, and 88.9% of total SIC, respectively. In contrast with soil organic carbon (SOC) storage, which is highest under 500–800 mm yr−1 of mean precipitation, SIC storage peaks where mean precipitation is <400 mm yr−1. The amount and vertical distribution of SIC was related to climate and land cover type. Content of SIC in each incremental horizon was positively related with mean annual temperature and negatively related with mean annual precipitation, with the magnitude of SIC content across land cover types showing the following order: desert, grassland >shrubland, cropland >marsh, forest, meadow. Densities of SIC increased generally with depth in all ecosystem types with the exception of deserts and marshes where it peaked in intermediate layers (0.1–0.3 m for first and 0.3–0.5 m for latter). Being an abundant component of soil carbon stocks in China, SIC dynamics and the process involved in its accumulation or loss from soils require a better understanding. 相似文献
13.
利用水蒸气蒸馏法提取小黄皮叶挥发油,运用毛细管气相色谱-质谱联用法对挥发油进行了分析,分离出40个峰,鉴定了其中的37种成分,所鉴定成分占挥发油总量的99.87%,其主要化学成分为单萜及倍半萜类化合物。 相似文献
14.
15.
肠道病毒71型作为引起儿童群体常见传染性手足口病(HFMD)的主要病原,具有导致少量感染个体出现脑炎等神经系统病变以及相关心肺功能衰竭的病理学特性.因此其预防性疫苗的研发具有重要的公共卫生意义.在前期工作的基础上,一种EV71灭活病毒疫苗(人二倍体细胞)在本研究中基于恒河猴婴猴模型进行了相应的免疫保护性分析.以160EU剂量对2~3月龄婴猴进行0,4周免疫后,动物在第4周接受了剂量为10。~CCID50的病毒经呼吸道的攻击.对病毒攻击后动物在14天内的临床症状、血液生物学、器官病原学分布以及病理学检测的动态观察表明,经疫苗免疫的动物未出现对照动物所具有的特征性临床表现,其血液生物学及病理学检测均无异常.同时,器官病原学分布亦呈阴性.结合动物中和抗体的明确增长及对照动物的综合表现分析,本文的工作证实了该EV71灭活病毒疫苗(人二倍体细胞)在恒河猴婴猴体内的免疫保护性. 相似文献
16.
17.
T. NGAMSIRI Y. OHASHI N. SUKUMASAVIN M. NAKAJIMA U. NA‐NAKORN N. TANIGUCHI 《Molecular ecology resources》2006,6(2):313-315
Microsatellite DNA markers for a critically endangered Mekong giant catfish (Pangasianodon gigas Roberts and Vidthayanon, 1991) were developed from fin clips collected from captive fish using (GT)15 probe. The number of alleles per locus ranged from two to four. The expected heterozygosities ranged from 0.13 to 0.68. Also, these primers were successfully amplified in four closely related species, Pangasius bocourti, Pangasius conchophilus, Pangasius larnaudii and Pangasius sanitwongsei with the number of alleles per locus ranged from 1 to 13, 1 to 16, 1 to 12 and 1 to 4, respectively. These markers should prove to be very useful for the evaluation of genetic diversity for this species and other related Pangasius species. 相似文献
18.
Shcherbakova NS Chikaev AN Karpenko LI Il'ichev AA 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2012,(1):20-25
The impact of monoclonal antibodies (mAb) biotinylation on the output and the repertoire of selected peptides in the biopanning procedure were tested. A comparative analysis of the peptides selected from phage library using the biotinylated and non-biotinylated mAb 2F5 was performed. It was shown that the output of peptides homologous to the native epitope was 1.7-fold higher for biotinylated antibodies, whereas the binding capacity of the selected phages with mAb 2F5 in ELISA was higher in the case of using non-biotinylated antibodies. It should be noted that the phages exposing peptides, which have 4-5 amino acid sequence similarity with the native epitope, demonstrate the highest binding affinity. The phages that expose peptides with 3 amino acid sequence similarity demonstrate different binding affinity: from the smallest to the largest. Based on the obtained data, it is safe to suggest that the rational biopanning may proceed in accordance with the task. 相似文献
19.
从茎花葱臭木种子中分离得到5个化合物,经理化与波谱分析鉴定为β-谷甾醇(1)、没食子酸乙酯(2)、胡萝卜苷(3)、1-O-β-D-吡喃葡萄糖基-(2S,3S,4R,8Z)-2-N-(2 ′-羟基二十四烷酰氨基)十八二氧鞘氨-8-烯(4)和2,3,2″,3″-四氢穗花杉双黄酮(5).这5个化合物均首次从该植物中分离得到.其中化合物5进行细胞毒活性测试,没有显示抑制活性. 相似文献
20.
从大苞藤黄枝叶的混合粉碎物中分离到11个化合物,运用光谱手段分别鉴定为neobractatin(1),brasixanthone B (2),5-O-methylxanthone V1 (3),10-O-methylmacluraxanthone (4),isobractatin (5),xanthone V1(6),xerophenone A (7),xerophenone B (8),bractatin (9),macluraxanthone (10)和3-O-methylneobractatin (11).本文首次应用超高效液相色谱-质谱联用技术分离了异构体7和8并测定了其精确分子量.其中化合物6~8为首次从该植物中发现. 相似文献