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81.
The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs)
to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold
in vitro was conducted using TGF-β 3 containing medium. After 3-weeks of induction, chondrocytic behavior, including marker
genes expression and specific extracellular matrix (ECM) secretion, was observed. In the in vivo evaluation experiment, the
scaffolds containing RBMSCs without prior induction were autologous implanted into the articular cartilage defects made by
subchondral drilling. The repairing ability was evaluated. After 2 months, chondrocyte-like cells with lacuna structure and
corresponding ECM were found in the repaired sites without apparent inflammation. After 24 weeks, we could easily find cartilage
structure the same with normal cartilage in the repair site. In conclusion, it was shown that the scaffolds in combination
of in vivo conditions can induce RBMSCs into chondrocytes in repaired area and would be a possible method for articular cartilage
repair in clinic and cartilage tissue engineering. 相似文献
82.
镜鲤体长性状的QTL定位分析 总被引:1,自引:0,他引:1
以镜鲤良种后代为祖父母本所培育的杂交F2群体的68个个体为材料,利用553个分子标记(217个SSR和336个SNP标记)对其进行基因型检测,运用JoinMap4.0软件包对遗传连锁图谱进行构建。利用MapQTL5.0区间作图法(Interval mapping)进行QTL检测,通过置换实验(1 000次重复)确定连锁群显著性水平阈值。在对体长的区间定位中共检测到12个与体长性状相关的QTLs区间,分布在BL-1-1(SNP0137-SNP1481)、BL-4-1(SNP0092-HLJ797)、BL-5-1(SNP1268-HLJ423)、BL-7-1(HLJ870-SNP0702)、BL-12-1(SNP0922-HLJ639)、BL-16-1(HLJE351-SNP0674)、BL-25-1(SNP0394-SNP0862)、BL-35-1(HLJ668-SNP0832)、BL-43-1(SNP0389-SNP1425)、BL-47-1(HLJ057-HLJ1113)、BL-47-2(HLJ1439-HLJ1418)等11个连锁群上,解释表型变异范围是13.8%~64.9%,其中贡献率大于20%的主效QTLs有8个,是体长性状的主效QTLs区间。 相似文献
83.
KCNQ1 variants associate with hypertension in type 2 diabetes and affect smooth muscle contractility in vitro 下载免费PDF全文
Kuo‐Chin Huang Te‐Mao Li Xiang Liu Jin‐Hua Chen Wen‐Kuei Chien Yi‐Tzone Shiao Hsinyi Tsang Ting‐Hsu Lin Chiu‐Chu Liao Shao‐Mei Huang Ju‐Pi Li Cheng‐Wen Lin Jung‐Chun Lin Chih‐Chien Lin Chih‐Ho Lai Chi‐Fung Cheng Wen‐Miin Liang Chien‐Hui Hung Ching‐Chu Chen Ying‐Ju Lin Fuu‐Jen Tsai 《Journal of cellular physiology》2017,232(12):3309-3316
84.
A novel, sensitive electrochemical immunoassay has been developed based on the precipitation of silver on colloidal gold labels which, after silver metal dissolution in an acidic solution, was indirectly determined by anodic stripping voltammetry (ASV) at a glassy-carbon electrode. The method was evaluated for a noncompetitive heterogeneous immunoassay of an immunoglobulin G (IgG) as a model. The influence of relevant experimental variables, including the reaction time of antigen with antibody, the dilution ratio of the colloidal gold-labeled antibody and the parameters of the anodic stripping operation, upon the peak current was examined and optimized. The anodic stripping peak current depended linearly on the IgG concentration over the range of 1.66 ng ml(-1) to 27.25 microg ml(-1) in a logarithmic plot. A detection limit as low as 1 ng ml(-1) (i.e., 6 x 10(-12) M) human IgG was achieved, which is competitive with colorimetric enzyme linked immuno-sorbent assay (ELISA) or with immunoassays based on fluorescent europium chelate labels. The high performance of the method is attributed to the sensitive ASV determination of silver (I) at a glassy-carbon electrode (detection limit of 5 x 10(-9) M) and to the catalytic precipitation of a large number of silver on the colloidal gold-labeled antibody. 相似文献
85.
Mark Hamann Chu The Cuong Nguyen Duy Hong Pham Thuoc Bui Thi Thuhien 《Biodiversity and Conservation》2006,15(11):3703-3720
To establish baseline data on the distribution, abundance and threats to marine turtles in Viet Nam we conducted surveys with
local fishers, community members and provincial Ministry of Fisheries staff from 17 of Viet Nam’s 29 coastal provinces. These
data indicate that five species of marine turtle reside in Viet Nam’s waters (loggerhead, olive ridley, leatherback, green
and hawksbill turtles), and four species nest on Viet Nam’s beaches (all of the above except the loggerhead turtle). It is
evident from these data that significant declines have occurred in both foraging and nesting populations of all five marine
turtle species found in Viet Nam. The greatest current threats to marine turtle populations in Viet Nam are habitat degradation,
the accidental and opportunistic of turtles capture by fishers and the direct take of nesting females and their eggs. Successful
conservation efforts have been made in recent years through collaboration between international Non Government Organisations
and several Vietnamese Government Ministries. Continued success of these projects and the development and implementation of
marine conservation policy will depend upon building awareness among Government employees, fishers and the general public
about marine turtle biology, ecology, and the need to protect them. 相似文献
86.
Chu GH Gu M Cassel JA Belanger S Stabley GJ DeHaven RN Conway-James N Koblish M Little PJ DeHaven-Hudkins DL Dolle RE 《Bioorganic & medicinal chemistry letters》2006,16(3):645-648
A novel series of phenylamino acetamide derivatives was synthesized. These amides were shown to be potent and selective kappa opioid receptor agonists. 相似文献
87.
88.
Mingzhu Luo Rong Fan Xiaoming Wang Junyu Lu Ping Li Wenbin Chu Yonghe Hu Xuewei Chen 《Bioscience reports》2021,41(6)
Ethnopharmacological relevance: Gualou Xiebai Banxia (GLXBBX) decoction is a well-known traditional Chinese herbal formula that was first discussed in the Synopsis of the Golden Chamber by Zhang Zhongjing in the Eastern Han Dynasty. In traditional Chinese medicine, GLXBBX is commonly prescribed to treat cardiovascular diseases, such as coronary heart disease and atherosclerosis.Objective: The present study aimed to examine GLXBBX’s preventative capacity and elucidate the potential molecular mechanism of Poloxamer 407 (P407)-induced hyperlipidemia in rats.Materials and methods: Both the control and model groups received pure water, and the test group also received a GLXBBX decoction. For each administration, 3 ml of the solution was administered orally. To establish hyperlipidemia, a solution mixed with 0.25 g/kg P407 dissolved in 0.9% normal saline was injected slowly into the abdominal cavity. At the end of the study, the rats’ plasma lipid levels were calculated using an automatic biochemical analyzer to evaluate the preventative capability of the GLXBBX decoction, and the serum and liver of the rats were collected.Results: The GLXBBX decoction significantly improved P407-induced hyperlipidemia, including increased plasma triglycerides (TGs), aspartate aminotransferase (AST) elevation, and lipid accumulation. Moreover, GLXBBX decoction treatment increased lipoprotein lipase (LPL) activity and mRNA expression of LPL. Furthermore, GLXBBX significantly suppressed the mRNA expression of stearoyl-CoA desaturase (SCD1).Conclusion: GLXBBX significantly improved P407-induced hyperlipidemia, which may have been related to enhanced LPL activity, increased LPL mRNA expression, and decreased mRNA expression of SCD1. 相似文献
89.
Chu CC Pinter PJ Henneberry TJ Umeda K Natwick ET Wei YA Reddy VR Shrepatis M 《Journal of economic entomology》2000,93(4):1329-1337
During 1996, 1997, and 1999, studies were conducted in cotton, sugar beets, alfalfa, yardlong bean, and peanut fields to compare insect catches in CC traps equipped with different trap base colors. The studies were conducted in southwestern United States, China, and India. The nine colors, white, rum, red, yellow, lime green, spring green, woodland green (dark green), true blue, and black, varied in spectral reflectance in the visible (400-700 nm) and near-infrared (700-1050 nm) portions of spectrum. Lime green, yellow, and spring green were the three most attractive trap base colors for silverleaf whitefly, Bemisia argentifolii Bellows & Perring, and leafhopper, Empoasca spp. adults. The three trap base colors were moderately high in the green, yellow, and orange spectral regions (490-600 nm), resembling the spectral reflectance curve of the abaxial (underleaf) surfaces of green cotton leaves. True blue and white were the most attractive trap base colors for western flower thrips, Frankliniella occidentalis (Pergande), adults. The true blue and white trap bases were moderately high in the blue spectral region (400-480 nm). 相似文献
90.
Adjacent transfer RNAs (tRNAs) in the A- and P-sites of the ribosome are in dynamic equilibrium between two different conformations called classical and hybrid states before translocation. Here, we have used single-molecule fluorescence resonance energy transfer to study the effect of Mg(2+) on tRNA dynamics with and without an acetyl group on the A-site tRNA. When the A-site tRNA is not acetylated, tRNA dynamics do not depend on [Mg(2+)], indicating that the relative positions of the substrates for peptide-bond formation are not affected by Mg(2+). In sharp contrast, when the A-site tRNA is acetylated, Mg(2+) lengthens the lifetime of the classical state but does not change the lifetime of the hybrid state. Based on these findings, the classical state resembles a state with direct stabilization of tertiary structure by Mg(2+) ions whereas the hybrid state resembles a state with little Mg(2+)-assisted stabilization. The antibiotic viomycin, a translocation inhibitor, suppresses tRNA dynamics, suggesting that the enhanced fluctuations of tRNAs after peptide-bond formation drive spontaneous attempts at translocation by the ribosome. 相似文献