全文获取类型
收费全文 | 3166篇 |
免费 | 217篇 |
出版年
2024年 | 3篇 |
2023年 | 25篇 |
2022年 | 63篇 |
2021年 | 98篇 |
2020年 | 45篇 |
2019年 | 76篇 |
2018年 | 92篇 |
2017年 | 77篇 |
2016年 | 120篇 |
2015年 | 202篇 |
2014年 | 194篇 |
2013年 | 214篇 |
2012年 | 340篇 |
2011年 | 270篇 |
2010年 | 168篇 |
2009年 | 160篇 |
2008年 | 202篇 |
2007年 | 208篇 |
2006年 | 164篇 |
2005年 | 144篇 |
2004年 | 113篇 |
2003年 | 93篇 |
2002年 | 105篇 |
2001年 | 12篇 |
2000年 | 10篇 |
1999年 | 20篇 |
1998年 | 11篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1995年 | 14篇 |
1994年 | 3篇 |
1993年 | 11篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 9篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 8篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1979年 | 3篇 |
1978年 | 8篇 |
1977年 | 2篇 |
1976年 | 4篇 |
1974年 | 3篇 |
1972年 | 3篇 |
1917年 | 1篇 |
排序方式: 共有3383条查询结果,搜索用时 15 毫秒
141.
Chiara Salvesi Stefania Silvi Dennis Fiorini Serena Scortichini Gianni Sagratini Francesco A. Palermo Renato De Leone Nadaniela Egidi Lorella Fatone Carlo Cifani Amedeo Amedei Francesca Scocchera Mara Morici Beatrice Gatto Fausto Mannucci Valerio Valeriani Marco Malavasi Sara Servili Andrea Casula Andrea Cresci Ivano Corradetti Francesco Carpi Matteo Picciolini Maria Magdalena Coman Maria Cristina Verdenelli 《Journal of applied microbiology》2022,133(5):2941-2953
142.
Orefice Ida Di Dato Valeria Sardo Angela Lauritano Chiara Romano Giovanna 《Aquatic Ecology》2022,56(2):377-397
Aquatic Ecology - Diatoms are eukaryotic microalgae representing one of the major groups in the marine phytoplankton, accounting for up to 40% of annual productivity at sea. They are widely... 相似文献
143.
Roveta Camilla Annibaldi Anna Domenichelli Federico Gregorin Chiara Gridelli Stefano Pantano Valentina Vagnoni Flavio Puce Stefania 《Aquatic Ecology》2022,56(3):631-637
Aquatic Ecology - Jellyfish blooms are an increasingly common event in our seas. Occurring via polyps’ asexual reproduction induced by human stresses, they represent a hazard for ecosystems... 相似文献
144.
145.
Thomas Noe Perry Hager Souabni Chiara Rapisarda Rémi Fronzes Fabrice Giusti Jean-Luc Popot Manuela Zoonens Francesca Gubellini 《生物化学与生物物理学报:生物膜》2019,1861(2):466-477
Membrane protein (MP) complexes play key roles in all living cells. Their structural characterisation is hampered by difficulties in purifying and crystallising them. Recent progress in electron microscopy (EM) have revolutionised the field, not only by providing higher-resolution structures for previously characterised MPs but also by yielding first glimpses into the structure of larger and more challenging complexes, such as bacterial secretion systems. However, the resolution of pioneering EM structures may be difficult and their interpretation requires clues regarding the overall organisation of the complexes. In this context, we present BAmSA, a new method for localising transmembrane (TM) regions in MP complexes, using a general procedure that allows tagging them without resorting to neither genetic nor chemical modification. Labels bound to TM regions can be visualised directly on raw negative-stain EM images, on class averages, or on three-dimensional reconstructions, providing a novel strategy to explore the organisation of MP complexes. 相似文献
146.
Allan D. McDevitt Naiara Guimarães Sales Samuel S. Browett Abbie O. Sparnenn Stefano Mariani Owen S. Wangensteen Ilaria Coscia Chiara Benvenuto 《Journal of fish biology》2019,95(2):679-682
We focus on a case study along an English canal comparing environmental DNA (eDNA) metabarcoding with two types of electrofishing techniques (wade-and-reach and boom-boat). In addition to corroborating data obtained by electrofishing, eDNA provided a wider snapshot of fish assemblages. Given the semi-lotic nature of canals, we encourage the use of eDNA as a fast and cost-effective tool to detect and monitor whole fish communities. 相似文献
147.
Chiara Pagliarani Valentino Casolo Maryam Ashofteh Beiragi Silvia Cavalletto Ilenia Siciliano Andrea Schubert Maria Lodovica Gullino Maciej A. Zwieniecki Francesca Secchi 《Plant, cell & environment》2019,42(6):1775-1787
Some plant species are capable of significant reduction of xylem embolism during recovery from drought despite stem water potential remains negative. However, the functional biology underlying this process is elusive. We subjected poplar trees to drought stress followed by a period of recovery. Water potential, hydraulic conductivity, gas exchange, xylem sap pH, and carbohydrate content in sap and woody stems were monitored in combination with an analysis of carbohydrate metabolism, enzyme activity, and expression of genes involved in sugar metabolic and transport pathways. Drought resulted in an alteration of differential partitioning between starch and soluble sugars. Upon stress, an increase in the starch degradation rate and the overexpression of sugar symporter genes promoted the efflux of disaccharides (mostly maltose and sucrose) to the apoplast. In turn, the efflux activity of the sugar‐proton cotransporters caused a drop in xylem pH. The newly acidic environment induced the activity of apoplastic invertases leading to the accumulation of monosaccharides in the apoplast, thus providing the main osmoticum necessary for recovery. During drought and recovery, a complex network of coordinated molecular and biochemical signals was activated at the interface between xylem and parenchyma cells that appeared to prime the xylem for hydraulic recovery. 相似文献
148.
149.
Giulia Paiardi Stefan Richter Pasqua Oreste Chiara Urbinati Marco Rusnati Rebecca C. Wade 《The Journal of biological chemistry》2022,298(2)
Heparin, a naturally occurring glycosaminoglycan, has been found to have antiviral activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus of COVID-19. To elucidate the mechanistic basis for the antiviral activity of heparin, we investigated the binding of heparin to the SARS-CoV-2 spike glycoprotein by means of sliding window docking, molecular dynamics simulations, and biochemical assays. Our simulations show that heparin binds at long, positively charged patches on the spike glycoprotein, thereby masking basic residues of both the receptor-binding domain (RBD) and the multifunctional S1/S2 site. Biochemical experiments corroborated the simulation results, showing that heparin inhibits the furin-mediated cleavage of spike by binding to the S1/S2 site. Our simulations showed that heparin can act on the hinge region responsible for motion of the RBD between the inactive closed and active open conformations of the spike glycoprotein. In simulations of the closed spike homotrimer, heparin binds the RBD and the N-terminal domain of two adjacent spike subunits and hinders opening. In simulations of open spike conformations, heparin induces stabilization of the hinge region and a change in RBD motion. Our results indicate that heparin can inhibit SARS-CoV-2 infection by three mechanisms: by allosterically hindering binding to the host cell receptor, by directly competing with binding to host heparan sulfate proteoglycan coreceptors, and by preventing spike cleavage by furin. Furthermore, these simulations provide insights into how host heparan sulfate proteoglycans can facilitate viral infection. Our results will aid the rational optimization of heparin derivatives for SARS-CoV-2 antiviral therapy. 相似文献
150.
INTEGRATE: Model-based multi-omics data integration to characterize multi-level metabolic regulation