全文获取类型
收费全文 | 2007篇 |
免费 | 170篇 |
国内免费 | 2篇 |
专业分类
2179篇 |
出版年
2022年 | 18篇 |
2021年 | 28篇 |
2020年 | 18篇 |
2019年 | 22篇 |
2018年 | 21篇 |
2017年 | 28篇 |
2016年 | 57篇 |
2015年 | 98篇 |
2014年 | 103篇 |
2013年 | 146篇 |
2012年 | 164篇 |
2011年 | 145篇 |
2010年 | 100篇 |
2009年 | 81篇 |
2008年 | 112篇 |
2007年 | 88篇 |
2006年 | 83篇 |
2005年 | 76篇 |
2004年 | 79篇 |
2003年 | 66篇 |
2002年 | 56篇 |
2001年 | 55篇 |
2000年 | 49篇 |
1999年 | 37篇 |
1998年 | 21篇 |
1997年 | 21篇 |
1996年 | 26篇 |
1995年 | 11篇 |
1994年 | 24篇 |
1993年 | 12篇 |
1992年 | 24篇 |
1991年 | 28篇 |
1990年 | 19篇 |
1989年 | 12篇 |
1988年 | 18篇 |
1987年 | 17篇 |
1986年 | 17篇 |
1985年 | 15篇 |
1984年 | 9篇 |
1983年 | 16篇 |
1982年 | 13篇 |
1981年 | 11篇 |
1980年 | 11篇 |
1979年 | 20篇 |
1978年 | 10篇 |
1976年 | 19篇 |
1975年 | 12篇 |
1974年 | 6篇 |
1973年 | 8篇 |
1970年 | 8篇 |
排序方式: 共有2179条查询结果,搜索用时 15 毫秒
21.
The two-state recurrent stochastic model with time-independent transition rates is generalized to a model with time-dependent transition rates. The rates can be any general function of external time, that is, any general function of the calendar time in which the process unfolds. Formulas for the state transition probabilities, the proportion of individuals in a particular state at time t, the distribution function, and the expectation of the number of individuals in a particular state at time t are derived. 相似文献
22.
The seasonal histories and phenological relationships of European corn borer,Ostrinia nubilalis (Hübner), and its 2 parasitoids,Macrocentrus grandii (Goidanich) andEriborus terebrans (Gravenhorst) were studied in southcentral Minnesota. Both parasitoids overwintered in mature borer larvae, broke diapause, completed development, and emerged at the same time as did borer adults. Thus the 1st generation parasitoids coincided with the peak abundance of their preferred larval instars of the 1st host generation. Both parasitoids had a 2nd generation, matching the bivoltinism ofO. nubilatis in Minnesota. The activity of 2nd generationE. terebrans was before the peak 2nd generation borer moth flight and was not synchronized with the peak abundance of 2nd generation borer larvae. The peak activity of 2nd generationM. grandii occurred after the peak 2nd generation borer moth flight and was fully synchronized with the peak abundance of 2nd generation borer larvae. Thus,M. grandii has both generations synchronized with the host seasonal history, and was the more effective of the 2 parasitoids. 相似文献
23.
Seven ribosome-binding sites on DNA have been located within the region defined by the nin5 deletion as well as several ribosome-binding sites on each side of the nin5 region. These were mapped by electron microscopy relative to the end points of the nin5 deletion and two Tn903 transposons, one inserted into gene Rz and another inserted near gene Q. These ribosomes binding sites within the nin5 region may correspond to polypeptide initiation sites for up to seven new dispensible lambda genes. 相似文献
24.
Anti-viral activity of 3-deazaadenosine and 5'-deoxy-5'-isobutylthio-3-deazaadenosine (3-deaza-SIBA) 总被引:1,自引:0,他引:1
A J Bodner G L Cantoni P K Chiang 《Biochemical and biophysical research communications》1981,98(2):476-481
3-Deazaadenosine and 5′-deoxy-5′-isobutylthio-3-deazaadenosine (3-deaza-SIBA) inhibits replication of both herpes simplex type 1 virus and the RNA type C virus, HL-23. Oncogenic transformation caused by SV40 and HL-23 are also blocked by either compound. Both compounds exhibit relatively low cytotoxicity at the anti-viral concentrations. 相似文献
25.
26.
E S Kang R E Gates T M Chiang A H Kang 《Biochemical and biophysical research communications》1979,86(3):769-778
Intact adipocytes exhibit ectoprotein kinase activity as reflected by their ability to catalyze the transfer of the terminal phosphate of (γ-32P) ATP to histone added to a cell suspension. This activity is substrate, time and cell number dependent. Lineweaver-Burk plots gave Km and Vmax values for ATP of 5 × 10?5 M and 7.14 pmoles/min/1.5 × 105 cells. Cyclic AMP but not cyclic GMP in μM concentrations stimulates ectoprotein kinase activity. The controlled tryptic digestion of intact cells results in reduction of ectoprotein kinase activity. This activity is not due to leakage of intracellular protein kinases during the preparative procedure nor to penetration of histone into the cells. Additional phosphoproteins not accessible to endogenous protein kinase activity are also localized on the external surface of the intact fat cell. 相似文献
27.
Guanidoacetate methyltransferase has been purified about 140-fold from pig liver. Polyacrylamide gel electrophoresis of the purified enzyme showed four protein bands, each of which is associated with guanidoacetate methyltransferase activity. During gel electrophoresis at pH 3 in 8 M urea, guanidoacetate methyltransferase migrated as a single component. The molecular weight of the purified guanidoacetate methyltransferase was estimated to be 31,000 by sodium dodecyl sulfate-gel electrophoresis, which also showed only one protein component with guanidoacetate methyltransferase activity. This molecular weight is in agreement with that estimated by Sephadex G-75 chromatography. Guanidoacetate methyltransferase is inhibited by adenosylhomocysteine, 3-deazaadenosylhomocysteine, and sinefungin with Ki values of 16 microM, 39 microM, and 18 microM, respectively. 相似文献
28.
29.
Edward J. Leonard Alison Skeel Peter K. Chiang Giulio L. Cantoni 《Biochemical and biophysical research communications》1978,84(1):102-109
An inhibitor of adenosylhomocysteine hydrolase, 3-deazaadenosine, caused profound inhibition of phagocytosis of opsonized erythrocytes by mouse resident peritoneal macrophages . The inhibition was evident at concentrations as low as 2×10?7M, and increased with increasing concentration and time of exposure to the analogue. It was not associated with detachment of the macrophage monolayers or with loss of cell viability. Although the inhibition was not reversible, progression of the functional impairment was interrupted by washing out the analogue. In striking contrast, phagocytic function of human blood monocytes was unaffected by 3-deazaadenosine. 相似文献
30.
The oxidation-reduction potential of chloroperoxidase, an enzyme which catalyzes peroxidative chlorination, bromination, and iodination reactions, has been investigated. In addition to catalyzing biological halogenation reactions, chloroperoxidase is unusual in that the carbon monoxide complex of ferrous chloroperoxidase shows the typical long wavelength Soret absorption associated with P-450 hemoproteins. The pH dependence of the chloroperoxidase oxidation-reduction potential shows a discontinuity around pH 4.7. Similarly, measurements of the affinity of ferrous chloroperoxidase for carbon monoxide monitored both by spectroscopic and potentiometric titration exhibit a discontinuity in the pH 4.7 region. Oxidation-reduction potential measurements on chloroperoxidase in a CO atmosphere also show a discontinuous pH profile. These results suggest that ferrous chloroperoxidase undergoes reversible modification at low pH and that these changes are reflected in the oxidation-reduction potential. The oxidation-reduction potential of chloroperoxidase at pH 6.9 is - 140 mV, close to that measured for cytochrome P-450cam in the presence of substrate. The oxidation-reduction potential of chloroperoxidase at pH 2.7, the pH optimum for enzymatic chlorination, is +150 mV. The oxidation-reduction potentials of the halide complexes of chloroperoxidase (chloride, bromide, and iodide) are essentially identical with the potential measurements on the native enzyme. These observations suggest that, although halide anions bind to the enzyme, they probably do not bind as an axial ligand to the heme ferric iron. 相似文献