全文获取类型
收费全文 | 796篇 |
免费 | 147篇 |
国内免费 | 4篇 |
专业分类
947篇 |
出版年
2023年 | 4篇 |
2022年 | 13篇 |
2021年 | 28篇 |
2020年 | 20篇 |
2019年 | 36篇 |
2018年 | 51篇 |
2017年 | 23篇 |
2016年 | 25篇 |
2015年 | 53篇 |
2014年 | 50篇 |
2013年 | 43篇 |
2012年 | 58篇 |
2011年 | 64篇 |
2010年 | 53篇 |
2009年 | 29篇 |
2008年 | 33篇 |
2007年 | 26篇 |
2006年 | 26篇 |
2005年 | 31篇 |
2004年 | 27篇 |
2003年 | 30篇 |
2002年 | 19篇 |
2001年 | 10篇 |
2000年 | 16篇 |
1999年 | 12篇 |
1997年 | 9篇 |
1996年 | 7篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 10篇 |
1987年 | 9篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 10篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 8篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 8篇 |
1974年 | 10篇 |
1973年 | 5篇 |
1970年 | 2篇 |
1968年 | 2篇 |
排序方式: 共有947条查询结果,搜索用时 0 毫秒
881.
882.
883.
884.
Neurogenesis and asymmetric cell division 总被引:1,自引:0,他引:1
The astonishing cellular diversity in the central nervous system (CNS) arises from neural progenitors which can undergo different modes of symmetric and asymmetric divisions to self-renew as well as produce differentiated neuronal and glial progeny. Drosophila CNS neural progenitor cells, neuroblasts, have been utilised as a model to stimulate the understanding of the processes of asymmetric division, generation of neuronal lineages and, more recently, stem cell biology in vertebrates. Here we review some recent developments involving Drosophila and mammalian neural progenitor cells, highlighting some similarities and differences in the mechanisms that regulate their divisions during neurogenesis. 相似文献
885.
Short‐Term Hypoxia Reverses Ox‐LDL‐Induced CD36 and GLUT4 Switching Metabolic Pathways in H9c2 Cardiomyoblast Cells 下载免费PDF全文
886.
Rodney T. Richardson Hailey R. Curtis Emma G. Matcham Chia‐Hua Lin Sreelakshmi Suresh Douglas B. Sponsler Luke E. Hearon Reed M. Johnson 《Molecular ecology》2019,28(3):686-697
We explored the pollen foraging behaviour of honey bee colonies situated in the corn and soybean dominated agroecosystems of central Ohio over a month‐long period using both pollen metabarcoding and waggle dance inference of spatial foraging patterns. For molecular pollen analysis, we developed simple and cost‐effective laboratory and bioinformatics methods. Targeting four plant barcode loci (ITS2, rbcL, trnL and trnH), we implemented metabarcoding library preparation and dual‐indexing protocols designed to minimize amplification biases and index mistagging events. We constructed comprehensive, curated reference databases for hierarchical taxonomic classification of metabarcoding data and used these databases to train the metaxa 2 DNA sequence classifier. Comparisons between morphological and molecular palynology provide strong support for the quantitative potential of multi‐locus metabarcoding. Results revealed consistent foraging habits between locations and show clear trends in the phenological progression of honey bee spring foraging in these agricultural areas. Our data suggest that three key taxa, woody Rosaceae such as pome fruits and hawthorns, Salix, and Trifolium provided the majority of pollen nutrition during the study. Spatially, these foraging patterns were associated with a significant preference for forests and tree lines relative to herbaceous land cover and nonflowering crop fields. 相似文献
887.
888.
889.
890.
Natriuretic peptides stimulate the cardiac sodium pump via NPR-C-coupled NOS activation 总被引:1,自引:0,他引:1
William M Hamilton EJ Garcia A Bundgaard H Chia KK Figtree GA Rasmussen HH 《American journal of physiology. Cell physiology》2008,294(4):C1067-C1073
Natriuretic peptides (NPs) and their receptors (NPRs) are expressed in the heart, but their effects on myocyte function are poorly understood. Because NPRs are coupled to synthesis of cGMP, an activator of the sarcolemmal Na(+)-K(+) pump, we examined whether atrial natriuretic peptide (ANP) regulates the pump. We voltage clamped rabbit ventricular myocytes and identified electrogenic Na(+)-K(+) pump current (arising from the 3:2 Na(+):K(+) exchange and normalized for membrane capacitance) as the shift in membrane current induced by 100 micromol/l ouabain. Ten nanomoles per liter ANP stimulated the Na(+)-K(+) pump when the intracellular compartment was perfused with pipette solutions containing 10 mmol/l Na(+) but had no effect when the pump was at near maximal activation with 80 mmol/l Na(+) in the pipette solution. Stimulation was abolished by inhibition of cGMP-activated protein kinase with KT-5823, nitric oxide (NO)-activated guanylyl cyclase with 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ), or NO synthase with N(G)-nitro-L-arginine methyl ester (L-NAME). Since synthesis of cGMP by NPR-A and NPR-B is not NO dependent or ODQ sensitive, we exposed myocytes to AP-811, a highly selective ligand for the NPR-C "clearance" receptor. It abolished ANP-induced pump stimulation. Conversely, the selective NPR-C agonist ANP(4-23) reproduced stimulation. The stimulation was blocked by l-NAME. To examine NO production in response to ANP(4-23), we loaded myocytes with the NO-sensitive fluorescent dye diacetylated diaminofluorescein-2 and examined them by confocal microscopy. ANP(4-23) induced a significant increase in fluorescence, which was abolished by L-NAME. We conclude that NPs stimulate the Na(+)-K(+) pump via an NPR-C and NO-dependent pathway. 相似文献