全文获取类型
收费全文 | 1327篇 |
免费 | 85篇 |
国内免费 | 1篇 |
出版年
2024年 | 2篇 |
2023年 | 6篇 |
2022年 | 12篇 |
2021年 | 24篇 |
2020年 | 15篇 |
2019年 | 19篇 |
2018年 | 24篇 |
2017年 | 20篇 |
2016年 | 47篇 |
2015年 | 80篇 |
2014年 | 83篇 |
2013年 | 103篇 |
2012年 | 118篇 |
2011年 | 117篇 |
2010年 | 78篇 |
2009年 | 60篇 |
2008年 | 83篇 |
2007年 | 83篇 |
2006年 | 78篇 |
2005年 | 77篇 |
2004年 | 69篇 |
2003年 | 60篇 |
2002年 | 50篇 |
2001年 | 18篇 |
2000年 | 14篇 |
1999年 | 10篇 |
1998年 | 13篇 |
1997年 | 7篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1965年 | 2篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有1413条查询结果,搜索用时 15 毫秒
101.
Germ cells and somatic cells have the identical genome. However, unlike the mortal fate of somatic cells, germ cells have the unique ability to differentiate into gametes that retain totipotency and produce an entire organism upon fertilization. The processes by which germ cells differentiate into gametes, and those by which gametes become embryos, involve dramatic cellular differentiation accompanied by drastic changes in gene expression, which are tightly regulated by genetic circuitries as well as epigenetic mechanisms. Epigenetic regulation refers to heritable changes in gene expression that are not due to changes in primary DNA sequence. The past decade has witnessed an ever-increasing understanding of epigenetic regulation in many different cell types/tissues during embryonic development and adult homeostasis. In this review, we focus on recent discoveries of epigenetic regulation of germ cell differentiation in various metazoan model organisms, including worms, flies, and mammals. 相似文献
102.
103.
104.
Yu Suk Choi Gregory J. Dusting Samantha Stubbs Sandeep Arunothayaraj Xiao Lian Han Philippe Collas Wayne A. Morrison Rodney J. Dilley 《Journal of cellular and molecular medicine》2010,14(4):878-889
Human adipose‐derived stem cells (ASCs) may differentiate into cardiomyocytes and this provides a source of donor cells for tissue engineering. In this study, we evaluated cardiomyogenic differentiation protocols using a DNA demethylating agent 5‐azacytidine (5‐aza), a modified cardiomyogenic medium (MCM), a histone deacetylase inhibitor trichostatin A (TSA) and co‐culture with neonatal rat cardiomyocytes. 5‐aza treatment reduced both cardiac actin and TropT mRNA expression. Incubation in MCM only slightly increased gene expression (1.5‐ to 1.9‐fold) and the number of cells co‐expressing nkx2.5/sarcomeric α‐actin (27.2%versus 0.2% in control). TSA treatment increased cardiac actin mRNA expression 11‐fold after 1 week, which could be sustained for 2 weeks by culturing cells in cardiomyocyte culture medium. TSA‐treated cells also stained positively for cardiac myosin heavy chain, α‐actin, TropI and connexin43; however, none of these treatments produced beating cells. ASCs in non‐contact co‐culture showed no cardiac differentiation; however, ASCs co‐cultured in direct contact co‐culture exhibited a time‐dependent increase in cardiac actin mRNA expression (up to 33‐fold) between days 3 and 14. Immunocytochemistry revealed co‐expression of GATA4 and Nkx2.5, α‐actin, TropI and cardiac myosin heavy chain in CM‐DiI labelled ASCs. Most importantly, many of these cells showed spontaneous contractions accompanied by calcium transients in culture. Human ASC (hASC) showed synchronous Ca2+ transient and contraction synchronous with surrounding rat cardiomyocytes (106 beats/min.). Gap junctions also formed between them as observed by dye transfer. In conclusion, cell‐to‐cell interaction was identified as a key inducer for cardiomyogenic differentiation of hASCs. This method was optimized by co‐culture with contracting cardiomyocytes and provides a potential cardiac differentiation system to progress applications for cardiac cell therapy or tissue engineering. 相似文献
105.
106.
Guo Hua Liang Seonghee Park Moon Young Kim Ji Aee Kim Shinkyu Choi Suk Hyo Suh 《Life sciences》2010,86(19-20):733-739
AimsThis study examined the effects of oxidized low-density lipoprotein (LDL) and its major lipid constituent lysophosphatidylcholine (LPC) on nonselective cation (NSC) current and its inhibitory contribution to LPC-induced cytotoxicity in cultured human umbilical endothelial cells (HUVECs).Main methodsPatch-clamp technique and the resazurin-based cell viability assay were used.Key findingsIn voltage-clamped cells, oxidized LDL or LPC slowly activated NSC current. NSC current was also activated by loading cells with Ca2+ solution buffered at various concentrations using a patch pipette or by applying the sarcoplasmic reticulum Ca2+ pump blocker 2,5-di-t-butyl-1,4-benzohydroquinone (BHQ), the metabolic inhibitor CN? or the hydroperoxide donor tert-butyl hydroperoxide (TBHP). On the contrary, when intracellular Ca2+ was strongly buffered with 12 mM BAPTA or cells were loaded with superoxide dismutase using a patch pipette, LPC or BHQ did not activate NSC current. Furthermore, NSC current activated by LPC, TBHP or CN? was inhibited by the antioxidant tempol or extracellular Ca2+ depletion and NSC current activated by intracellular Ca2+ was further augmented by oxidized LDL or LPC. LPC or oxidized LDL released Ca2+ from intracellular stores and further enhanced store-operated Ca2+ entry. LPC-induced cytotoxicity was augmented by inhibiting Ca2+ influx and NO synthesis.SignificanceOxidized LDL or its main component LPC activated Ca2+-permeable NSC current via releasing Ca2+ from intracellular stores and producing ROS and thereby increased Ca2+ influx. Ca2+ influx through NSC channel might protect endothelial cells by producing NO. 相似文献
107.
108.
Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs 总被引:1,自引:0,他引:1
Two closely related Arabidopsis mitogen-activated protein kinases (MAPKs), MPK3 and MPK6, are rapidly but transiently activated in plants exposed to ozone. Although the contribution of these MAPKs to control of redox stress has been examined extensively, it remains unclear whether the dual-specificity MKPs play an essential role in the regulation of these processes. To explore this question, specific knockdown of each of the five putative MKPs in Arabidopsis was performed, and the ozone sensitivity phenotype of each MKP-suppressed line was assessed. Silencing of only one previously uncharacterized MKP, designated AtMKP2, rendered the plants hypersensitive to oxidative stress. AtMKP2-suppressed plants displayed significantly prolonged MPK3 and MPK6 activation during ozone treatment, and recombinant AtMKP2 was able to dephosphorylate both phospho-MPK3 and phospho-MPK6 in vitro, providing direct evidence that AtMKP2 may target these oxidant-activated MAPKs. In addition, the in vitro phosphatase activity of AtMKP2 was enhanced by co-incubation with either recombinant MPK3 or MPK6. In AtMKP2:YFP-expressing plants, the fusion protein was localized predominantly in the nucleus, the same compartment into which ozone-activated MPK3 and MPK6 have previously been shown to be translocated. Taken together, these data suggest that AtMKP2, a novel MKP protein in Arabidopsis, acts upon MPK3 and -6, and serves as a positive regulator of the cellular response to oxidant challenge. 相似文献
109.
110.
Adameová A Kuzelová M Andelová E Faberová V Pancza D Svec P Ziegelhöffer A Ravingerová T 《Molecular and cellular biochemistry》2007,295(1-2):129-136
Both, diabetes mellitus (DM) and hypercholesterolemia (HCH) are known as risk factors of ischemic heart disease, however,
the effects of experimental DM, as well as of HCH alone, on ischemia/reperfusion-induced myocardial injury are not unequivocal.
We have previously demonstrated an enhanced resistance to ischemia-induced arrhythmias in rat hearts in the acute phase of
DM. Our objectives were thus to extend our knowledge on how DM in combination with HCH, a model that is relevant to diabetic
patients with altered lipid metabolism, may affect the size of myocardial infarction and susceptibility to arrhythmias. A
combination of streptozotocin (STZ; 80 mg/kg, i.p.) and the fat–cholesterol diet (1% cholesterol, 1% coconut oil; FCHD) was
used as a double-disease model mimicking DM and HCH simultaneosly occurring in humans. Following 5 days after STZ injection
and FCHD leading to increased blood glucose and cholesterol levels, anesthetized open-chest diabetic, diabetic–hypercholesterolemic
(DM–HCH) and age-matched control rats were subjected to 6-min ischemia (occlusion of LAD coronary artery) followed by 10 reperfusion
to test susceptibility to ventricular arrhythmias in the in vivo experiments and to 30-min ischemia and subsequent 2-h reperfusion for the evaluation of the infarct size (IS) in the Langendorff-perfused
hearts. The incidence of the most life-threatening ventricular arrhythmia, ventricular fibrillation, was significantly increased
in the DM–HCH rats as compared with non-diabetic control animals (100% vs. 50%; p<0.05). Likewise, arrhythmia severity score (AS) was significantly higher in the DM–HCH rats than in the controls (4.9±0.2
vs. 3.5±0.5; p<0.05), but was not increased in the diabetic animals (AS 3.7±0.9; p>0.05 vs. controls). Diabetic hearts exhibited a reduced IS (15.1±3.0% of the area at risk vs. 37.6±2.8% in the control hearts;
p<0.05), however, a combination of DM and HCH increased the size of myocardial infarction to that observed in the controls.
In conclusion, HCH abrogates enhanced resistance to ischemia-reperfusion injury in the diabetic rat heart. 相似文献