全文获取类型
收费全文 | 180篇 |
免费 | 14篇 |
专业分类
194篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 4篇 |
2013年 | 6篇 |
2012年 | 3篇 |
2011年 | 6篇 |
2010年 | 6篇 |
2009年 | 2篇 |
2008年 | 6篇 |
2007年 | 4篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 6篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 4篇 |
1992年 | 3篇 |
1990年 | 2篇 |
1988年 | 3篇 |
1987年 | 7篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 8篇 |
1972年 | 6篇 |
1971年 | 5篇 |
1970年 | 2篇 |
1969年 | 4篇 |
1968年 | 6篇 |
1966年 | 1篇 |
1933年 | 1篇 |
排序方式: 共有194条查询结果,搜索用时 10 毫秒
81.
[See figures]. The synthesis of analogues of adenosine diphosphate ribose and acetylated adenosine diphosphate ribose, modified at the northern pentose, is reported. The stereochemistry at the acetylated centers was chosen to minimize acetyl migration and dictated the overall synthetic strategy. 相似文献
82.
N. Billotte N. Marseillac P. Brottier J.‐L. Noyer J.‐P. Jacquemoud‐Collet C. Moreau T. Couvreur M.‐H. Chevallier J.‐C. Pintaud A.‐M. Risterucci 《Molecular ecology resources》2004,4(2):256-258
A (GA)n microsatellite‐enriched library was constructed and 16 nuclear simple sequence repeat (SSR) loci were characterized in Phoenix dactylifera. Across‐taxa amplification and genotyping tests showed the utility of most SSR markers in 11 other Phoenix species and the transferability of some of them in Elaeis guineensis, 11 species of Pritchardia, Pritchardiopsis jeanneneyi and six species of Astrocaryum. The first to be published for P. dactylifera, these new SSR resources are available for cultivar identification, pedigree analysis, germplasm diversity as well as genetic mapping studies. 相似文献
83.
84.
Glucocorticoids (GCs) modulate insulin-like growth factor action in cartilage through mechanisms that are complex and insufficiently defined, especially in the context of cranio-facial growth. Because the family of IGF-binding proteins (IGFBP-1 to -6) is important in the regulation of IGF availability and bioactivity, we examined the effect of GCs on chondrocyte differentiation in correlation with IGFBP production in cultured fetal rat chondrocytes isolated from nasal septum cartilage of fetal rat. Dexamethasone (DEX) effects were tested before and at the onset of extracellular matrix maturation. DEX induced a dose-dependent increase in the size of cartilage nodule formed, (45)Ca incorporation into extracellular matrix, alkaline phosphatase activity, and sulfatation of glycosaminoglycans, maximal effects being obtained with a 10-mM DEX concentration. The IGFBPs produced by cultured chondrocytes were characterized in culture medium which had been conditioned for 24 h under serum-free conditions by these cells. Western ligand blotting with a mixture of [(125)I]IGF-I and -II revealed bands of 20, 24, 29, a 31-32 kDa doublet and a 39-41 kDa triplet which were differently regulated by DEX. Immunoblotting showed that following DEX exposure, IGFBP-3 and -6 were up-regulated whereas IGFBP-2, -5, and the 24 kDa band were down-regulated. The effect of DEX on both differentiation and IGFBP production showed a same dependence, and developed when extracellular matrix maturation had been just induced. The results obtained in this chondrocyte culture system show that production of IGFBPs is modulated by DEX at physiological concentrations thus regulating IGF availability and action, a control which could promote the primordial role of the rat nasal septum in craniofacial growth. 相似文献
85.
Kobayashi T Beuchat MH Chevallier J Makino A Mayran N Escola JM Lebrand C Cosson P Kobayashi T Gruenberg J 《The Journal of biological chemistry》2002,277(35):32157-32164
Very little is known about the biophysical properties and the lipid or protein composition of membrane domains presumably present in endocytic and biosynthetic organelles. Here we analyzed the membrane composition of late endosomes by suborganellar fractionation in the absence of detergent. We found that the internal membranes of this multivesicular organelle can be separated from the limiting membrane and that each membrane population exhibited a defined composition. Our data also indicated that internal membranes may consist of at least two populations, containing primarily phosphatidylcholine or lysobisphosphatidic acid as major phospholipid, arguing for the existence of significant microheterogeneity within late endosomal membranes. We also found that lysobisphosphatidic acid exhibited unique pH-dependent fusogenic properties, and we speculated that this lipid is an ideal candidate to regulate the dynamic properties of this internal membrane mosaic. 相似文献
86.
Role of the proteasome in Alzheimer's disease 总被引:4,自引:0,他引:4
Checler F da Costa CA Ancolio K Chevallier N Lopez-Perez E Marambaud P 《Biochimica et biophysica acta》2000,1502(1):133-138
The proteasome is a multicatalytic complex involved in the degradation of polyubiquitinated proteins. Here we review the clues of a possible involvement of the proteasome in Alzheimer's disease neuropathology. Thus, we discuss the fact that the proteasome modulates the intracellular concentrations of presenilins 1 and 2. These two proteins, when mutated, appear responsible for most of early onset forms of Alzheimer's disease and this is thought to be due to the exacerbation of the pathogenic pathway of the maturation of the beta-amyloid precursor protein. Controlling presenilins concentrations could have drastic repercussions on cell physiology as suggested by the fact that proteasome inhibitors drastically potentiate the 'normal' or 'pathogenic' presenilins phenotype related with betaAPP processing. The possibility of considering the proteasome as a potential target for therapeutic intervention in Alzheimer's disease is discussed. 相似文献
87.
Laetitia Magnol Marie-Clémence Chevallier Valérie Nalesso Stéphanie Retif Helmut Fuchs Martina Klempt Patricia Pereira Michel Riottot Sandra Andrzejewski Bich-Thuy Doan Jean-Jacques Panthier Anne Puech Jean-Claude Beloeil Martin Hrabe de Angelis Yann Hérault 《BMC developmental biology》2007,7(1):1-15
Background
Targeted disruption of the murine 3β-hydroxysterol-Δ7-reductase gene (Dhcr7), an animal model of Smith-Lemli-Opitz syndrome, leads to loss of cholesterol synthesis and neonatal death that can be partially rescued by transgenic replacement of DHCR7 expression in brain during embryogenesis. To gain further insight into the role of non-brain tissue cholesterol deficiency in the pathophysiology, we tested whether the lethal phenotype could be abrogated by selective transgenic complementation with DHCR7 expression in the liver.Results
We generated mice that carried a liver-specific human DHCR7 transgene whose expression was driven by the human apolipoprotein E (ApoE) promoter and its associated liver-specific enhancer. These mice were then crossed with Dhcr7+/- mutants to generate Dhcr7-/- mice bearing a human DHCR7 transgene. Robust hepatic transgene expression resulted in significant improvement of cholesterol homeostasis with cholesterol concentrations increasing to 80~90 % of normal levels in liver and lung. Significantly, cholesterol deficiency in brain was not altered. Although late gestational lung sacculation defect reported previously was significantly improved, there was no parallel increase in postnatal survival in the transgenic mutant mice.Conclusion
The reconstitution of DHCR7 function selectively in liver induced a significant improvement of cholesterol homeostasis in non-brain tissues, but failed to rescue the neonatal lethality of Dhcr7 null mice. These results provided further evidence that CNS defects caused by Dhcr7 null likely play a major role in the lethal pathogenesis of Dhcr7 -/- mice, with the peripheral organs contributing the morbidity. 相似文献88.
Two bovine brain-derived growth factors, BDGF I and BDGF II, were isolated using the same extraction procedure as previously described for eye-derived growth factors (EDGF). The hypothesis that these growth factors were identical to EDGF I and EDGF II, respectively, was supported by their similar molecular weights (16,000 and 15,000, respectively) and isoelectric points (9.0 and 5.0, respectively), their identical retention behavior on reverse-phase chromatography and their similar amino acid compositions. From studies on their binding properties to cell surfaces, competition between EDGF I and BDGF I as well as competition between EDGF II and BDGF II to the same receptor was observed. The amino terminal sequence of EDGF II (1-16) was shown to be identical to the amino acid residues (7-22) of the acidic FGF, strongly confirming our observations on the identity of the factors isolated from bovine brain and retina. 相似文献
89.
Pierre Peterlin Camille Debord Marion Eveillard Alice Garnier Amandine Le Bourgeois Thierry Guillaume Maxime Jullien Marie C. Bn Patrice Chevallier 《Journal of cellular and molecular medicine》2022,26(21):5486
Myeloid Derived Suppressive Cells (MDSC) are capable to suppress innate and adaptive immune responses, thus favouring solid cancer progression. However, little is known about the role of MDSC in acute myeloid leukaemia (AML). In this monocentric prospective study, 73 adult AML patients, eligible for first‐line intensive chemotherapy, were included with the aim to study the influence on long‐term outcomes of peripheral blood (PB) levels of monocytic (M) MDSC (M‐MDSC) assessed by flow cytometry. A percentage of peripheral M‐MDSC higher than 0.55% of leukocytes at diagnosis and a decrease of M‐MDSC% after induction came out both as independent negative prognostic factors for leukaemia‐free and overall survival. 相似文献
90.
Régine Vivien Soraya Saïagh Philippe Lemarre Valérie Chabaud Béline Jesson Catherine Godon Ulrich Jarry Thierry Guillaume Patrice Chevallier Henri Vié Béatrice Clémenceau 《Cytotherapy》2018,20(3):436-452