人胎胸腺细胞的异质性荧光及其与T淋巴细胞分化阶段关系的形态学研究

以７６９０－Ｘｕ荧光染色法结合ＷｕＴ３、ＷｕＴ４、ＷｕＴ８致敏红细胞花环实验观察经胸腺细胞分层液分离所得高密度亚群和低密度亚群人胎胸腺细胞的异质性荧光及其膜分化抗原ＣＤ３、ＣＤ４、ＣＤ８的表达。结果表明：不同胎龄胎儿胸腺细胞悬液呈现相似形态和相似分布特征的８种异质性荧光的细胞,其中,墨黑核细胞和桔红核细胞分布于两密度亚群,表型为ＣＤ３－ＣＤ４－ＣＤ８－;少数深蓝核和蓝核细胞分布于低密度亚群．表型为ＣＤ３＋,属成熟的胸腺细胞;大多数深蓝核和蓝核细胞、灰蓝核细胞、灰黄核细胞及部分淡桔黄核细胞分布于高密度亚群,表型为ＣＤ３－、ＣＤ４＋、ＣＤ８＋,为处于中间发育阶段的胸腺细胞。推测这些细胞胞核由深蓝、蓝、灰蓝、灰黄到淡桔黄的荧光光谱的偏移可能与中间发育阶段所发生的生物及理化变化有关。     

东方法老蛛雌蛛的描述（蜘蛛目：跳蛛科）

报道采自甘肃文县的东方法老蛛雌蛛,为该种雌蛛的首次记述。     

鳗鱼肌肉的氨基酸及营养价值

通过对优质食用鱼类—鳗鱼肌肉的氨基酸进行测定证实,鳗鱼较之其它鱼类是一种营养价值更高、滋味更鲜美的鱼类。并且,根据结果氨基酸组成比例,可为鳗鱼的人工饲养等方面的研究提供理论依据。     

复合氨基酸治疗眼角膜溃疡的研究

复合氨基酸在临床上的应用越来越被人们重视,我们利用水解猪血粉的方法得到含１７种氨基酸的复合液,配制成滴眼剂用于多种原因引起的眼角膜溃疡疾病的治疗研究取得了较好的效果。     

用中性红标记酵母原生质体初探

用２％蜗牛酶处理酵母细胞６０分钟,啤酒酵母Ｙ２９的原生质体形成率为９０％,再生率为９．５％;糖化酵母ＩＢ的原生质体形成率为８６％,再生率为１２％。用５００ｐｐｍ中性红染液对Ｙ２９菌株的整细胞和原生质体染色１５分钟,细胞的着色率为８４％,存活率为１２％,而原生质体的着色率为７５％,再生率为６．４％,经染色后的原生质体体积缩小,在交变电场中排队所需的场强电降低。     

以淀粉珠为载体的亲和层析法分离纯化高温α淀粉酶

 以淀粉珠为载体的亲和层析法分离纯化高温α淀粉酶张学忠,宋伦,王群,吴晓霞,唐锌进（吉林大学酶工程国家重点实验室,长春１３００２３;南京师范大学生物系,南京２１００２４）金凤燮等人从酒曲中筛选出高产热稳定α淀粉酶的菌株,命名为Ｂａｃｉｌｌｕｓｓｐ－ＪＦ...     

Bi（Ⅲ）与金属硫蛋白作用性质研究

 Ｂｉ（Ⅲ）与金属硫蛋白作用性质研究张保林,黄辉,朱凌燕,岳晟,唐雯霞（南京大学配位化学研究所,配位化学国家重点实验室,南京２１００９３）如何降低顺铂或其它抗癌铂的毒性,一直是癌症化疗中的重要课题之一,最近研究发现预先给大鼠或肺癌病人服用铋盐,可以极大...     

湿地松粉蚧夏季数量凋落的原因分析

利用生命表技术及其相应的控制指数分析方法,对新侵入害虫湿地松粉蚧Ｏｒａｏｅｌｌａａ－ｃｕｔａ（Ｌｏｂｄｅｌｌ）夏季数量凋落的某些因子进行了量化分析。结果表明：在广东南部的新侵入区,夏季高温引起的松梢迅速老化,上代为害以后引起的营养质量的变化、拥挤以及煤污病的严重发生等,均对湿地松粉蚧夏季种群数量的凋落有着明显的影响,其总的排除作用控制指数ＥＩＰＣ为４６．８９,如果排除这几个因子的作用,下代种群的数量将为当代的４６．８９倍。     

Phylogenetic relationships among eutherian orders estimated from inferred sequences of mitochondrial proteins: Instability of a tree based on a single gene

The phylogenetic relationships among Primates (human), Artiodactyla (cow), Cetacea (whale), Carnivora (seal), and Rodentia (mouse and rat) were estimated from the inferred amino acid sequences of the mitochondrial genomes using Marsupialia (opossum), Aves (chicken), and Amphibia (Xenopus) as an outgroup. The overall evidence of the maximum likelihood analysis suggests that Rodentia is an outgroup to the other four eutherian orders and that Cetacea and Artiodactyla form a clade with Carnivora as a sister taxon irrespective of the assumed model for amino acid substitutions. Although there remains an uncertainty concerning the relation among Artiodactyla, Cetacea, and Carnivora, the existence of a clade formed by these three orders and the outgroup status of Rodentia to the other eutherian orders seems to be firmly established. However, analyses of individual genes do not necessarily conform to this conclusion, and some of the genes reject the putatively correct tree with nearly 5% significance. Although this discrepancy can be due to convergent or parallel evolution in the specific genes, it was pointed out that, even without a particular reason, such a discrepancy can occur in 5% of the cases if the branching among the orders in question occurred within a short period. Due to uncertainty about the assumed model underlying the phylogenetic inference, this can occur even more frequently. This demonstrates the importance of analyzing enough sequences to avoid the danger of concluding an erroneous tree.     

Capsid assembly and involved function analysis of twelve core protein mutants of duck hepatitis B virus.

The roles of different regions of the duck hepatitis B virus (DHBV) core protein on viral capsid assembly and related functions were examined. Twelve deletion and insertion mutations which covered 80% of the DHBV C open reading frame were constructed and expressed in Escherichia coli. The N-terminal region (amino acids 3 to 66) of DHBV core protein was important for its tertiary structure and function in E. coli. The expressed core mutants without this region apparently inhibited E. coli growth. The results of transmission electron microscopy of E. coli thin sections, capsid agarose gel, and sucrose gradient sedimentation demonstrated that a few DHBV core mutants with insertion in the N terminus and deletion in the C terminus retained the ability to form core-like particles in E. coli. However, other mutations in most of N-terminal and central regions strongly inhibited the self-assembly ability of DHBV core protein in E. coli. In addition, the mutant with a C-terminal region deletion (amino acids 181 to 228) lost most of the nucleic acid-binding activity of the DHBV core protein.