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A.D. Shutov H. Braun Yu.V. Chesnokov Ch. Horstmann I.A. Kakhovskaya H. Bäumlein 《Journal of molecular evolution》1998,47(4):486-492
The development of seeds as a specialized organ for the nutrition, protection, and dispersal of the next generation was an
important step in the evolution of land plants. Seed maturation is accompanied by massive synthesis of storage compounds such
as proteins, starch, and lipids. To study the processes of seed storage protein evolution we have partially sequenced storage
proteins from maturing seeds of representatives from the gymnosperm genera Gnetum, Ephedra, and Welwitschia—morphologically diverse and unusual taxa that are grouped in most formal systems into the common order Gnetales. Based on
partial N-terminal amino acid sequences, oligonucleotide primers were derived and used for PCR amplification and cloning of
the corresponding cDNAs. We also describe the structure of the nuclear gene for legumin of Welwitschia mirabilis. This first gnetalean nuclear gene structure contains introns in only two of the four conserved positions previously characterized
in other spermatophyte legumin genes. The distinct phylogenetic status of the gnetalean taxa is also reflected in a sequence
peculiarity of their legumin genes. A comparative analysis of exon/intron sequences leads to the hypothesis that legumin genes
from Gnetales belong to a monophyletic evolutionary branch clearly distinct from that of legumin genes of extant Ginkgoales
and Coniferales as well as from all angiosperms.
Received: 5 June 1997 / Accepted: 31 March 1998 相似文献
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QTL Mapping of Diffuse Reflectance Indices of Leaves in Hexaploid Bread Wheat (Triticum aestivum L.)
Chesnokov Yu. V. Kanash E. V. Mirskaya G. V. Kocherina N. V. Rusakov D. V. Lohwasser U. Börner A. 《Russian Journal of Plant Physiology》2019,66(1):77-86
Russian Journal of Plant Physiology - Quantitative trait loci (QTL) mapping of diffuse reflectance indices of laminas in bread wheat (Triticum aestivum L.) has been first performed under controlled... 相似文献
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Proteomic and bioinformatics profile of paired human alveolar macrophages and peripheral blood monocytes 下载免费PDF全文
Kathleen C. Lundberg Jessica Jarvela Gurkan Bebek Nicole G. Chesnokov Daniela Schlatzer W. Henry Boom Mark R. Chance Richard F. Silver 《Proteomics》2015,15(22):3797-3805
Little is known about proteomic differences between pluripotent human peripheral blood monocytes (MN) and their terminally‐differentiated pulmonary counterparts, alveolar macrophages (AM). To better characterize these cell populations, we performed a label‐free shotgun proteomics assessment of matched AM and MN preparations from eight healthy volunteers. With an FDR of less than 0.45%, we identified 1754 proteins within AM and 1445 from MN. Comparison of the two proteomes revealed that 1239 of the proteins found in AM were shared with MN, whereas 206 proteins were uniquely identified in MN and 515 were unique to AM. Molecular and cellular functions, protein classes, development associations, and membership in physiological systems and canonical pathways were identified among the detected proteins. Analysis of biologic processes represented by these proteomes indicated that MN were most prominently enriched for proteins involved in cellular movement and immune cell trafficking. In contrast, AM were enriched for proteins involved in protein trafficking, molecular transport, and cellular assembly and organization. These findings provide a baseline proteomic resource for further studies aimed at better understanding of the functional differences between MN and AM in both health and disease. 相似文献
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EG Ciolac SS Mantuani CM Neiva CEL Verardi DM Pess?a-Filho L Pimenta 《Biology of sport / Institute of Sport》2015,32(2):103-108
The aim of the present study was to analyse the usefulness of the 6-20 rating of perceived exertion (RPE) scale for prescribing and self-regulating high-intensity interval training (HIT) in young individuals. Eight healthy young subjects (age = 27.5±6.7 years) performed maximal graded exercise testing to determine their maximal and reserve heart rate (HR). Subjects then performed two HIT sessions (20 min on a treadmill) prescribed and regulated by their HR (HR: 1 min at 50% alternated with 1 min at 85% of reserve HR) or RPE (RPE: 1 minute at the 9-11 level [very light-fairly light] alternated with 1 minute at the 15-17 level [hard-very hard]) in random order. HR response and walking/running speed during the 20 min of exercise were compared between sessions. No significant difference between sessions was observed in HR during low- (HR: 135±15 bpm; RPE: 138±20 bpm) and high-intensity intervals (HR: 168±15 bpm; RPE: 170±18 bpm). Walking/running speed during low- (HR: 5.7±1.2 km · h−1; RPE: 5.7±1.3 km · h−1) and high-intensity intervals (HR: 7.8±1.9 km · h−1; RPE: 8.2±1.7 km · h−1) was also not different between sessions. No significant differences were observed in HR response and walking/running speed between HIT sessions prescribed and regulated by HR or RPE. This finding suggests that the 6-20 RPE scale may be a useful tool for prescribing and self-regulating HIT in young subjects. 相似文献